Frontiers in Microbiology,
Journal Year:
2022,
Volume and Issue:
13
Published: July 26, 2022
Comammox,
as
a
newly
discovered
ammonia
oxidizer,
urgently
needs
highly
efficient
and
specific
primers
to
detect
its
community
structure
diversity.
In
this
study,
the
performance
of
widely
used
primer
set
Ntsp-amoA
162F/359R
designed
sets
comamoA
F/R,
CA377f/C576r,
CB377f/C576r
were
evaluated,
for
high-throughput
sequencing
comammox
amoA
genes
in
natural
arable
soils
sampled
from
two
locations
black
soil
region
northeast
China.
Results
showed
that,
compared
with
had
more
advantages
detecting
operational
taxonomic
unit
(OTU)
numbers,
diversity,
structure.
The
CA377f/C576r
an
advantage
sequences
low
relative
abundance.
addition,
results
phylogenetic
tree
abundance
dominant
OTUs
that
China
was
dominated
by
Nitrospira
Clade
B.
Furthermore,
our
study
found
long-term
land
use
reduced
alpha
diversity
community,
but
lead
convergent
evolution
Mantel
test
canonical
correspondence
analysis
indicated
NO3--N
content
most
important
factor
affecting
comammox.
Our
provided
experience
accumulation
selection
Critical Reviews in Environmental Science and Technology,
Journal Year:
2022,
Volume and Issue:
53(2), P. 161 - 176
Published: March 20, 2022
The
discovery
of
complete
ammonia
oxidizers,
comammox
Nitrospira,
represents
a
breakthrough
in
the
history
nitrification
research.
Nitrospira
inopinata,
which
was
obtained
from
an
aquatic
ecosystem,
is
only
pure
bacterial
isolate
reported
so
far.
Ammonia
oxidation
kinetics
N.
inopinata
indicated
that
they
prefer
oligotrophic
lifestyle
and
may
directly
compete
with
ammonia-oxidizing
archaea
(AOA),
but
representatives
terrestrial
ecosystems
are
lacking.
Current
studies
demonstrate
functionally
dominant
separated
most
sequences
engineering
systems.
Clades
A
B
seemingly
have
different
ecological
preferences
soils,
possibly
due
to
their
ammonium
uptake
Analyses
evolutionary
indicate
some
genes
involved
could
be
laterally
transferred
β-ammonia-oxidizing
bacteria
(AOB).
Some
species
similar
AOB
active
niches
where
previously
considered
dominated
by
AOB.
Taken
together,
this
review
summarizes
recent
findings
biogeographical
distribution
preference
large-scale
soil
surveys,
responses
nitrogen
application
soil,
putative
mechanisms
underpinning
niche
Nitrospira.
These
suggest
not
strictly
both
copiotrophic
broader
breadth.
Comammox
abundant
environments.
profoundly
expanded
our
knowledge
environmental
relative
contribution
soils.
Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(2), P. 436 - 436
Published: Feb. 14, 2022
Biochar
has
been
widely
recognized
as
an
effective
and
eco-friendly
ameliorant
for
saline
soils,
but
information
about
the
mechanism
of
how
biochar
influences
nitrification
in
salt-affected
agroecosystem
remains
fragmented.
An
incubation
experiment
was
performed
on
soil
collected
from
a
three-consecutive-year
at
application
gradients
7.5
t⋅ha−1,
15
t⋅ha−1
30⋅t
ha−1
under
nitrogen
(N)
fertilization.
Responses
rate
(NR),
numbers
ammonia
monooxygenase
(amoA)
gene
copies,
community
structures
ammonia-oxidizing
bacteria
(AOB)
archaea
(AOA)
to
were
investigated.
The
results
indicated
that,
N
fertilization,
NR
amoA-AOB
amoA-AOA
copies
negatively
responded
addition.
increased
diversity
AOB
decreased
that
AOA.
addition
fertilization
shifted
Nitrosospira-dominated
Nitrosospira
Nitrosomonas-dominated,
altered
AOA
Nitrososphaera-dominated
Nitrososphaera
Nitrosopumilus-dominated.
relative
abundance
Nitrosospira,
Nitrosomonas
Nitrosopumilus
decreased,
Nitrosovibrio
with
rate.
Soil
SOC,
pH
NO3−-N
explained
87.1%
variation
community,
78.1%
explanatory
by
SOC.
SOC
influenced
through
Nitrosovibrio,
Nitrosomonas,
Norank_c_environmental_samples_p_Crenarchaeota
abundance.
Therefore,
inhibited
irrigation-silting
shifting
reducing
dominant
functional
ammonia-oxidizers,
such
Nitrosopumilus.
