Frontiers in Microbiology,
Journal Year:
2022,
Volume and Issue:
13
Published: July 26, 2022
Comammox,
as
a
newly
discovered
ammonia
oxidizer,
urgently
needs
highly
efficient
and
specific
primers
to
detect
its
community
structure
diversity.
In
this
study,
the
performance
of
widely
used
primer
set
Ntsp-amoA
162F/359R
designed
sets
comamoA
F/R,
CA377f/C576r,
CB377f/C576r
were
evaluated,
for
high-throughput
sequencing
comammox
amoA
genes
in
natural
arable
soils
sampled
from
two
locations
black
soil
region
northeast
China.
Results
showed
that,
compared
with
had
more
advantages
detecting
operational
taxonomic
unit
(OTU)
numbers,
diversity,
structure.
The
CA377f/C576r
an
advantage
sequences
low
relative
abundance.
addition,
results
phylogenetic
tree
abundance
dominant
OTUs
that
China
was
dominated
by
Nitrospira
Clade
B.
Furthermore,
our
study
found
long-term
land
use
reduced
alpha
diversity
community,
but
lead
convergent
evolution
Mantel
test
canonical
correspondence
analysis
indicated
NO3--N
content
most
important
factor
affecting
comammox.
Our
provided
experience
accumulation
selection
Agronomy,
Journal Year:
2023,
Volume and Issue:
13(4), P. 1144 - 1144
Published: April 17, 2023
The
use
of
biochar
and
lime
(CaO)
is
a
common
approach
to
mitigating
soil
acidification.
However,
little
known
about
how
amendments
impact
N2O
emissions
potential
microbial
mechanisms.
We
conducted
45-day
microcosm
incubation
experiment
examine
emission
associated
functional
guilds
amendment
in
an
acidic
tea
plantation
soil.
Results
show
that
treatments
significantly
reduced
cumulative
by
49.69%
63.01%,
respectively,
while
increasing
CO2
27.51%
19.35%,
respectively.
Additionally,
decreased
the
abundances
bacterial
nirK,
nirS,
nosZ
fungal
nirK
genes,
ammonia-oxidizing
bacteria
(AOB)
complete
(comammox)
amoA
genes.
stimulated
or
inhibitory
effects
on
genes
were
higher
than
lime.
rate
was
positively
linked
with
abundance
gene
but
negatively
correlated
AOB
comammox
abundances.
random
forest
linear
regression
analysis
revealed
denitrifiers
most
important
predictors
emissions.
Lime
alpha
diversity
altered
community
composition
nirK-harboring
denitrifiers.
Ascomycota
dominant
belonging
families
Nectriaceae,
Aspergillaceae,
Chaetomiaceae,
relative
genera
Chaetomium,
Penicillium
Fusarium
Overall,
our
findings
suggest
more
effective
reducing
emissions,
this
likely
due
powerful
it
has
traits
Frontiers in Microbiology,
Journal Year:
2021,
Volume and Issue:
12
Published: Oct. 5, 2021
Autotrophic
nitrification
is
regulated
by
canonical
ammonia-oxidizing
archaea
(AOA)
and
bacteria
(AOB)
nitrite-oxidizing
(NOB).
To
date,
most
studies
have
focused
on
the
role
of
ammonia
oxidizers
in
while
neglecting
NOB.
In
order
to
understand
impacts
combined
biochar
chemical
fertilizer
addition
associated
nitrifiers
plant
rhizosphere
soil,
we
collected
soil
from
a
maize
field
under
four
different
treatments:
no
fertilization
(CK),
(B),
nitrogen
(N)
+
phosphorus
(P)
potassium
(K)
fertilizers
(NPK),
NPK
(B
NPK).
The
potential
rate
(PNR),
community
abundances,
structures
AOA,
AOB,
complete
(Comammox
Nitrospira
clade
A),
Nitrobacter-
Nitrospira-like
NOB
were
measured.
Biochar
and/or
additions
increased
pH
nutrient
contents
soil.
B,
NPK,
B
treatments
significantly
stimulated
PNR
abundances
Comammox,
NOB,
with
highest
values
observed
treatment.
Pearson
correlation
random
forest
analyses
predicted
more
importance
Comammox
A,
over
AOA
PNR.
strongly
altered
whole
nitrifying
structures.
Redundancy
analysis
(RDA)
showed
that
affected
contents.
This
research
shows
application
has
positive
effect
improving
affecting
communities
AOB
These
new
revelations,
especially
as
they
related
understudied
can
be
used
increase
efficiency
agricultural
land
resource
management.
