Evaluation of four primer sets for analysis of comammox communities in black soils DOI Creative Commons
Xin Bai, Xiaojing Hu, Junjie Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: July 26, 2022

Comammox, as a newly discovered ammonia oxidizer, urgently needs highly efficient and specific primers to detect its community structure diversity. In this study, the performance of widely used primer set Ntsp-amoA 162F/359R designed sets comamoA F/R, CA377f/C576r, CB377f/C576r were evaluated, for high-throughput sequencing comammox amoA genes in natural arable soils sampled from two locations black soil region northeast China. Results showed that, compared with had more advantages detecting operational taxonomic unit (OTU) numbers, diversity, structure. The CA377f/C576r an advantage sequences low relative abundance. addition, results phylogenetic tree abundance dominant OTUs that China was dominated by Nitrospira Clade B. Furthermore, our study found long-term land use reduced alpha diversity community, but lead convergent evolution Mantel test canonical correspondence analysis indicated NO3--N content most important factor affecting comammox. Our provided experience accumulation selection

Language: Английский

Comammox dominate soil nitrification under different N fertilization regimes in semi-arid areas of Northeast China DOI Open Access

Xuewan Feng,

Meng Wang, Qian Li

et al.

Applied Soil Ecology, Journal Year: 2023, Volume and Issue: 193, P. 105119 - 105119

Published: Sept. 1, 2023

Language: Английский

Citations

7
Synergistically mitigating nitric oxide emission by co-applications of biochar and nitrification inhibitor in a tropical agricultural soil DOI
Changhua Fan,

Youfeng Leng,

Qi Zhang

et al.

Environmental Research, Journal Year: 2022, Volume and Issue: 214, P. 113989 - 113989

Published: Aug. 11, 2022

Language: Английский

Citations

11
Biochar enhancement of nitrification processes varies with soil conditions DOI Creative Commons
Lauren Hale,

Aileen Hendratna,

Natalie M. Scott

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 887, P. 164146 - 164146

Published: May 13, 2023

Language: Английский

Citations

6
Nine years of low-dose biochar amendment suppresses nitrification rate in low-yield brown soil DOI
Yue Li, Huanhuan Wei, Mingqi Liu

et al.

Applied Soil Ecology, Journal Year: 2023, Volume and Issue: 192, P. 105096 - 105096

Published: Aug. 17, 2023

Language: Английский

Citations

5
Evaluation of four primer sets for analysis of comammox communities in black soils DOI Creative Commons
Xin Bai, Xiaojing Hu, Junjie Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: July 26, 2022

Comammox, as a newly discovered ammonia oxidizer, urgently needs highly efficient and specific primers to detect its community structure diversity. In this study, the performance of widely used primer set Ntsp-amoA 162F/359R designed sets comamoA F/R, CA377f/C576r, CB377f/C576r were evaluated, for high-throughput sequencing comammox amoA genes in natural arable soils sampled from two locations black soil region northeast China. Results showed that, compared with had more advantages detecting operational taxonomic unit (OTU) numbers, diversity, structure. The CA377f/C576r an advantage sequences low relative abundance. addition, results phylogenetic tree abundance dominant OTUs that China was dominated by Nitrospira Clade B. Furthermore, our study found long-term land use reduced alpha diversity community, but lead convergent evolution Mantel test canonical correspondence analysis indicated NO3--N content most important factor affecting comammox. Our provided experience accumulation selection

Language: Английский

Citations

9