Phosphorus addition decreases plant lignin but increases microbial necromass contribution to soil organic carbon in a subalpine forest

Global Change Biology, Journal Year: 2022, Volume and Issue: 28(13), P. 4194 - 4210

Published: April 21, 2022

Increasing phosphorus (P) inputs induced by anthropogenic activities have increased P availability in soils considerably, with dramatic effects on carbon (C) cycling and storage. However, the underlying mechanisms via which drives plant microbial regulation of soil organic C (SOC) formation stabilization remain unclear, hampering accurate projection sequestration under future global change scenarios. Taking advantage an 8-year field experiment increasing addition levels a subalpine forest eastern Tibetan Plateau, we explored inputs, communities, biomarkers, as well SOC physical chemical fractions. We found that continuous reduced fine root biomass, but did not affect total content. decreased lignin contribution to SOC, primarily from declined vanillyl-type phenols, was coincided reduction methoxyl/N-alkyl 2.1%-5.5%. Despite decline decomposition due suppressed oxidase activity addition, content lignin-derived compounds because low input roots. In contrast, (mainly fungal) necromass its slower N-acquisition enzyme activity. The larger corresponded 9.1%-12.4% increase carbonyl abundance. Moreover, had no influence slow-cycing mineral-associated pool, stability indicated aliphaticity recalcitrance indices. Overall, over 8 years influenced composition through divergent alterations plant- microbial-derived contributions, shape stability. Such findings may aid accurately forecasting dynamics their potential feedbacks climate scenarios Earth system models.

Language: Английский

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Nitrogen fertilizer builds soil organic carbon under straw return mainly via microbial necromass formation

Soil Biology and Biochemistry, Journal Year: 2023, Volume and Issue: 188, P. 109223 - 109223

Published: Oct. 22, 2023

Language: Английский

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Reducing the uncertainty in estimating soil microbial-derived carbon storage

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(35)

Published: Aug. 22, 2024

Soil organic carbon (SOC) is the largest pool in terrestrial ecosystems and plays a crucial role mitigating climate change enhancing soil productivity. Microbial-derived (MDC) main component of persistent SOC pool. However, current formulas used to estimate proportional contribution MDC are plagued by uncertainties due limited sample sizes neglect bacterial group composition effects. Here, we compiled comprehensive global dataset employed machine learning approaches refine our quantitative understanding contributions total storage. Our efforts resulted reduction relative standard errors prevailing estimations an average 71% minimized effect variations compositions on estimating MDC. estimation indicates that contributes approximately 758 Pg, representing 40% stock. study updated with improving accuracy preserving simplicity practicality. Given unique biochemistry functioning pool, has direct implications for modeling predicting land–atmosphere balance under future scenarios.

Language: Английский

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Litter quality controls the contribution of microbial carbon to main microbial groups and soil organic carbon during its decomposition

Biology and Fertility of Soils, Journal Year: 2024, Volume and Issue: 60(2), P. 167 - 181

Published: Jan. 6, 2024

Language: Английский

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High litter quality enhances plant energy channeling by soil macro‐detritivores and lowers their trophic position

Ecology, Journal Year: 2025, Volume and Issue: 106(2)

Published: Feb. 1, 2025

Abstract Detritus‐based resources, that is, plant litter, are a major energy source for many living organisms and considered to be key determinant of primary production nutrient cycling. Earthworms among the most important macro‐detritivores in terrestrial food webs play crucial role facilitating these processes ecosystems. Yet, influence litter quality on earthworm nutrition, consequently soil web dynamics, has remained largely underexplored, mainly methodological reasons. Here, we combined bulk compound‐specific stable isotope analysis amino acids investigate dietary contribution different resources species ecological groups. Our findings show earthworms acquired essential from bacterial (~60%) (~30%) with latter increasing importance higher quality, resulting lower trophic positions across species. The high corresponds dominance bacteria experimental soil, suggesting served as an intermediate link transferring detritus‐based earthworms. Bacterial contributions were notably soil‐feeding than litter‐feeding species, likely due more pronounced ingestion by Overall, our study indicates group macro‐detritivores, earthworms, receive detrital via channel. Further, it underscores shaping niches detritivores, thereby influencing overall structure webs.

Language: Английский

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Nutrient (C, N and P) enrichment induces significant changes in the soil metabolite profile and microbial carbon partitioning

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 172, P. 108779 - 108779

Published: July 18, 2022

The cycling of soil organic matter (SOM) and carbon (C) within the is governed by presence key macronutrients, particularly nitrogen (N) phosphorus (P). relative ratio these nutrients has a direct effect on potential rates microbial growth nutrient processing in thus fundamental to ecosystem functioning. However, changing stoichiometry small molecule (i.e., metabolite) composition community remains poorly understood. Here, we aimed disentangle stoichiometrically balanced addition metabolomic profile apparent use efficiency (CUE) adding labile C source (glucose) combination with N and/or P. After incorporation added glucose into biomass (48 h), metabolite profiling was undertaken ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 494 metabolites were identified across all treatments mainly consisting lipids (n = 199), amino acids 118) carbohydrates 43), >97% which showed significant changes concentration between at least one treatment. Overall, glucose-C generally increased synthesis other soil, while together peptide synthesis, indicative protein formation turnover. P significantly number fatty synthesised. There no change PLFA-derived structure or following C, addition. Further, led an increase partitioning anabolic processes CUE), suggesting N, but not limited. Based profiles observed here, conclude that inorganic enrichment causes substantial shifts both primary secondary metabolism community, leading resource flow functioning, however, illustrated metabolic flexibility.

Language: Английский

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Hydrolyzable microplastics in soil—low biodegradation but formation of a specific microbial habitat?

Biology and Fertility of Soils, Journal Year: 2022, Volume and Issue: 58(4), P. 471 - 486

Published: March 31, 2022

Abstract Microplastics (MP, plastic particles between 0.1 and 5000 μm) contaminate agricultural soils through the application of organic fertilizers, sewage sludge, mulch. MP surfaces MP-soil interface provide specific habitats for soil microorganisms—the plastisphere. Microorganisms in plastisphere may benefit from utilizing as a carbon (C) source. Hydrolyzable with ester bonds are susceptible to enzymatic depolymerization by hydrolysis. In microcosm experiment, we investigated biodegradation small large (< 0.5 mm 0.5–2 respectively), hydrolyzable (a poly(lactic acid)/poly(butylene co-adipate terephthalate) blend, PLA/PBAT) non-hydrolyzable (low-density polyethylene, LDPE) polymers, effects these on microorganisms dry wet MP-amended soil. affected neither abundance composition main microbial groups (fungi, Gram-negative, Gram-positive bacteria), activities ß-glucosidase, ß-xylosidase, lipase, phenoloxidase, nor respiration Only PLA/PBAT were significantly mineralized (15.4% initial PLA/PBAT-C after 230 days). mineralization coincided enhanced lipase ß-glucosidase individual extracted incubation (compared LDPE non-incubated particles). We detected cracks using scanning electron microscopy, indicating initiation biodegradation, presumably due lipases. Results suggest that is polymer-specific habitat lipase-producing microorganisms. Our study demonstrates analyzing biogeochemical interactions within plastispheres essential assess fate their impacts microbially driven processes.

Language: Английский

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A critical review of the use of lipid signature molecules for the quantification of arbuscular mycorrhiza fungi

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 166, P. 108574 - 108574

Published: Jan. 29, 2022

Language: Английский

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Hydrogen stable isotope probing of lipids demonstrates slow rates of microbial growth in soil

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(16)

Published: April 10, 2023

The rate at which microorganisms grow and reproduce is fundamental to our understanding of microbial physiology ecology. While soil microbiologists routinely quantify biomass levels the growth rates individual taxa in culture, there a limited how quickly microbes actually soil. For this work, we posed simple question: what are microorganisms? In study, measure these three distinct environments using hydrogen-stable isotope probing lipids with 2H-enriched water. This technique provides taxa-agnostic quantification situ from degree 2H enrichment intact polar lipid compounds ascribed bacteria fungi. We find that quite slow correspond average generation times 14 45 d but also highly variable compound-specific level (4 402 d), suggesting differential among community subsets. observe low-biomass communities exhibit more rapid than high-biomass communities, highlighting quantity alone does not predict productivity Furthermore, within given soil, specific being synthesized do relate their quantity, general decoupling abundance microbiomes. More generally, demonstrate utility lipid-stable for measuring highlight importance complement standard analyses communities.

Language: Английский

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Increasing concentration of pure micro- and macro-LDPE and PP plastic negatively affect crop biomass, nutrient cycling, and microbial biomass

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 458, P. 131932 - 131932

Published: June 26, 2023

Over the last 50 years, intense use of agricultural plastic in form mulch films has led to an accumulation soil, creating a legacy fields. Plastic often contains additives, however it is still largely unknown how these compounds affect soil properties, potentially influencing or masking effects itself. Therefore, aim this study was investigate pure plastics varying sizes and concentrations, improve our understanding plastic-only interactions within soil-plant mesocosms. Maize (Zea mays L.) grown over eight weeks following addition micro macro low-density polyethylene polypropylene at increasing concentrations (equivalent 1, 10, 25, years film use) on key plant properties were measured. We found effect both microplastic health negligible short-term (1 <10 years). However, ≥ 10 application for types resulted clear negative growth microbial biomass. This provides vital insight into microplastics properties.

Language: Английский

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