Immunomodulatory effects of Lactiplantibacillus plantarum CCFM8661 + stachyose on cyclophosphamide-induced immunosuppression mice DOI Creative Commons
Weiwei Ma, Hang Sun, Lian Lian

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 27, 2025

Introduction The increasing stress of modern life has led to a decline in immunity, sparking widespread interest new strategies boost immune function. Lactiplantibacillus plantarum and stachyose have gained attention for their immune-regulating effects, but the mechanisms combined application remain unclear. This study aims investigate immunoregulatory effects CCFM8661 with cyclophosphamide-induced immunocompromised mice. Methods Mice were divided into normal control group, model group (normal saline), positive drug (levamisole hydrochloride, 10 mg/kg), low, medium, high-dose groups (1.5×10 5 , 1.5×10 6 7 CFU + 1.5 mg stachyose). Each treatment received continuous oral gavage administration 28 days. On days 23 24, except all other intraperitoneally injected cyclophosphamide (40 mg/kg) establish an immunosuppressed model. day 28, 30 minutes after final administration, mice euthanized collect thymus, spleen, serum, ileum, feces subsequent analysis indicators, intestinal barrier function, serum cytokines, microbiota. Results combination significantly improved organ atrophy, restored morphology, normalized cytokine levels mice, indicating enhanced Additionally, it microbiota diversity by abundance Muribaculaceae while reducing Lachnospiraceae potentially promoting homeostasis. Discussion immune-enhancing achieved regulating inflammation maintaining

Language: Английский

Immunomodulatory effects of Lactiplantibacillus plantarum CCFM8661 + stachyose on cyclophosphamide-induced immunosuppression mice DOI Creative Commons
Weiwei Ma, Hang Sun, Lian Lian

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 27, 2025

Introduction The increasing stress of modern life has led to a decline in immunity, sparking widespread interest new strategies boost immune function. Lactiplantibacillus plantarum and stachyose have gained attention for their immune-regulating effects, but the mechanisms combined application remain unclear. This study aims investigate immunoregulatory effects CCFM8661 with cyclophosphamide-induced immunocompromised mice. Methods Mice were divided into normal control group, model group (normal saline), positive drug (levamisole hydrochloride, 10 mg/kg), low, medium, high-dose groups (1.5×10 5 , 1.5×10 6 7 CFU + 1.5 mg stachyose). Each treatment received continuous oral gavage administration 28 days. On days 23 24, except all other intraperitoneally injected cyclophosphamide (40 mg/kg) establish an immunosuppressed model. day 28, 30 minutes after final administration, mice euthanized collect thymus, spleen, serum, ileum, feces subsequent analysis indicators, intestinal barrier function, serum cytokines, microbiota. Results combination significantly improved organ atrophy, restored morphology, normalized cytokine levels mice, indicating enhanced Additionally, it microbiota diversity by abundance Muribaculaceae while reducing Lachnospiraceae potentially promoting homeostasis. Discussion immune-enhancing achieved regulating inflammation maintaining

Language: Английский

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