Hearing Research, Journal Year: 2023, Volume and Issue: 436, P. 108823 - 108823
Published: June 2, 2023
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 126773 - 126773
Published: Sept. 9, 2023
Language: Английский
Citations
23
RNA Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 30, 2025
Viroids, small circular non-coding RNAs, act as infectious pathogens in higher plants, demonstrating high stability despite consisting solely of naked RNA. Their dependence replication on host machinery poses the question whether RNA modifications play a role viroid biology. Here, we explore avocado sunblotch (ASBVd) and citrus exocortis (CEVd), representative members viroids replicating chloroplasts nucleus, respectively, using LC – MS Oxford Nanopore Technology (ONT) direct sequencing. Although no modification was detected ASBVd, CEVd contained approximately one m6A per molecule. ONT sequencing predicted three positions. Employing orthogonal SELECT method, confirmed two positions A353 A360, which are highly conserved among variants. These located left terminal region rod-like structure where likely Pol II TFIIIA-7ZF bind, thus suggesting potential biological methylation replication.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 15, 2025
Abstract Functional CD8 + T cell immunity is essential for immune surveillance and host defense against infection tumors. Epigenetic mechanisms, particularly RNA modification, in controlling response not fully elucidated. Here, by cell-specific deletion of fat mass obesity-associated protein (FTO), a critical N6-methyladenosine (m 6 A) demethylase, we revealed that FTO was indispensable adequate protective function. ablation led to considerable death activated cells, which attributed apoptosis. MeRIP-seq analysis an increase m A methylation on Fas mRNA FTO-deficient cells. The loss promoted expression via enhancing the stability, depended reader insulin-like growth factor-2 mRNA-biding proteins 3 (IGF2BP3). Mutation sites or knockdown IGF2BP3 could normalize upregulated apoptosis levels caused Our findings delineate novel epigenetic regulatory mechanism FTO-mediated modification supporting survival effector responses, providing new insights into understanding post-transcriptional regulation immunological functions potential therapeutic intervention.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 12, 2024
Clear cell renal carcinoma (ccRCC) is characterized by a high incidence and mortality rate. Despite advancements in therapeutic interventions, the prognosis for cancer patients remains suboptimal. Of late, methylation modifications have emerged as promising molecular targets tumor assessment treatment, yet their potential has not been fully investigated context of ccRCC. Transcriptomic clinical data were extracted from The Cancer Genome Atlas, Gene Expression Omnibus, ArrayExpress databases, leading to identification 57 methylation-related genes (MRGs). Utilizing DESeq2 analysis, Cox regression LASSO algorithm, Methylation-Related Risk Score (MARS) was constructed. Cluster Ontology (GO) feature immune infiltration mutation analysis further employed evaluate model. Our investigation identified six pivotal prognostic MRGs established risk score predicated on m6A/m5C/m1A/m7G regulatory factors. This validated across two external cohorts can be utilized assess individual statuses predict responses immunotherapy. Moreover, cluster delineated distinct gene clusters. We developed robust signature based associated with m6A, m5C, m1A, m7G modifications. demonstrates significant value assessing survival outcomes, characteristics, infiltration, immunotherapy ccRCC patients. finding provides valuable insights refining precision treatment strategies.
Language: Английский
Citations
2
The Plant Journal, Journal Year: 2024, Volume and Issue: 120(5), P. 2014 - 2030
Published: Nov. 4, 2024
SUMMARY N 1 ‐methyladenosine (m A) methylation is an essential mechanism of gene regulation known to impact several biological processes in living organisms. However, little about the abundance, distribution, and functional significance mRNA m A modification during fruit ripening tomato main model species for fleshy fruits. Our study shows that modifications are prevalent detected lncRNA circRNA. The distribution peaks segments indicates mainly enriched at start codon CDS regions. Assessing changes global RNA wild‐type tomatoes ripening‐impaired Nr mutant affected ethylene receptor ( SlETR3 ) revealed a decrease overall levels from mature green (MG) stage 6 days postbreaker (Br + 6). fruits show significantly lower than Ailsa Craig (AC) Notably, differences well correlated expression number key ripening‐related genes. integration RNA‐seq MeRIP‐seq data suggests potential positive on expression. In comparison AC fruits, hypomethylation reduced ethylene‐related genes, ACO3 , EBF1 ERF.D6 mutants likely underpin distinct phenotypic traits observed between two genotypes Br6 stage. Overall, our brings further arguments supporting ripening, developmental process instrumental plant reproduction sensory nutritional qualities.
Language: Английский
Citations
1
Journal of Hepatocellular Carcinoma, Journal Year: 2023, Volume and Issue: Volume 10, P. 2383 - 2395
Published: Dec. 1, 2023
Introduction: RNA modifications mediated by the m6A, m1A, and m5C regulatory genes are crucial for progression of malignancy.This study aimed to explore expression regulator m6A/m5C/m1A methylation at single-cell level validate their in cancerous adjacent para-cancerous liver tissues adult patients with HCC who underwent tumor resection.Methods: The bulk sequencing from Cancer Genome Atlas (TCGA) database (scRNA-seq) data obtained Gene Expression Omnibus (GEO) were used identify dysregulated hepatocellular carcinoma (HCC).A real-time polymerase chain reaction (real-time PCR) was measure collected human compared tissues.Immune cell infiltration these significantly expressed methylation-related evaluated using Timer2.0.Results: A discrepancy gene observed between scRNA-seq.The clustered heatmap scRNA-seq-identified TCGA cohort revealed heterogeneous regulators within cancer, whereas more homogeneous.The PCR validated significant overexpression DNMT1, NSUN5, TRMT6, IGF2BP1, IGFBP3, which identified scRNA-seq, IGFBP2, sequencing.These mainly correlated natural killer cells.Discussion: This suggests that cellular diversity inside tumors contributes traditional scRNA-seq.This five will be focus further studies regarding function HCC.
Language: Английский
Citations
3
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 12, 2024
Viroids, small circular non-coding RNAs, act as infectious pathogens in higher plants, demonstrating high stability despite consisting solely of naked RNA. Their dependence replication on host machinery poses the question whether RNA modifications play a role viroid biology. Here, we explore avocado sunblotch (ASBVd) and citrus exocortis (CEVd), representative members viroids replicating chloroplasts nucleus, respectively, using LC–MS Oxford Nanopore Technology (ONT) direct sequencing. Although no modification was detected ASBVd, CEVd contained approximately one m 6 A per molecule. ONT sequencing predicted three positions. Employing orthogonal SELECT method, confirmed two positions A353 A360, which are highly conserved among variants. These postitions located left terminal region rod-like structure where likely Pol II TFIIIA-7ZF bind, thus suggesting potential biological methylation replication.
Language: Английский
Citations
0
Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117900 - 117900
Published: Aug. 5, 2024
Language: Английский
Citations
0