Replisome passage through the cohesin ring DOI Creative Commons
Samson Glaser, Maxim I. Molodtsov, John F.X. Diffley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Following eukaryotic genome replication, the two newly synthesised sister chromatids remain paired by ring-shaped cohesin complex, enabling their faithful segregation to daughter cells during cell divisions 1 . Cohesin topologically embraces DNA already before replication 2–5 , and replisome passage through ring is thought of as a fail-safe mechanism ensuring that entraps both products 6,7 Whether replisomes indeed pass rings remains unknown. Here, we use biochemical reconstitution 4,8,9 single molecule fluorescence microscopy directly visualise replisome-cohesin encounters. We find translocating replicative Cdc45-Mcm2-7-GINS (CMG) helicase frequently an obstacle cohesin, but likelihood with which CMG passes increases in presence components known chromatid cohesion functions 7,10–14 or preventing from freely sliding along template DNA. retains topological entrapment passage, suggesting ring. The frequency further when fully reconstituted encounter rings, resulting successful establishment between products. Our findings demonstrate existence simple links chromosome segregation, rings.

Language: Английский

Coordination of cohesin and DNA replication observed with purified proteins DOI
Yasuto Murayama,

Shizuko Endo,

Yumiko Kurokawa

et al.

Nature, Journal Year: 2024, Volume and Issue: 626(7999), P. 653 - 660

Published: Jan. 24, 2024

Language: Английский

Citations

17
Sister chromatid cohesion establishment during DNA replication termination DOI Creative Commons
George Cameron, Dominika T. Gruszka,

Rhian Gruar

et al.

Science, Journal Year: 2024, Volume and Issue: 384(6691), P. 119 - 124

Published: March 14, 2024

Newly copied sister chromatids are tethered together by the cohesin complex, but how chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that complexes, bound to before replication, remain behind advancing fork keep together. By visualizing single forks colliding preloaded we find replisome instead pushes where a converging met. Whereas replisomes removed during termination, remains on nascent and provides cohesion. Additionally, show CMG (CDC45-MCM2-7-GINS) helicase disassembly termination vital for proper in budding yeast. Together, our results support model wherein established termination.

Language: Английский

Citations

17
Sister chromatid cohesion through the lens of biochemical experiments DOI
Yasuto Murayama

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 93, P. 102464 - 102464

Published: Jan. 28, 2025

Language: Английский

Citations

0
Replisome passage through the cohesin ring DOI Creative Commons
Samson Glaser, Maxim I. Molodtsov, John F.X. Diffley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Following eukaryotic genome replication, the two newly synthesised sister chromatids remain paired by ring-shaped cohesin complex, enabling their faithful segregation to daughter cells during cell divisions 1 . Cohesin topologically embraces DNA already before replication 2–5 , and replisome passage through ring is thought of as a fail-safe mechanism ensuring that entraps both products 6,7 Whether replisomes indeed pass rings remains unknown. Here, we use biochemical reconstitution 4,8,9 single molecule fluorescence microscopy directly visualise replisome-cohesin encounters. We find translocating replicative Cdc45-Mcm2-7-GINS (CMG) helicase frequently an obstacle cohesin, but likelihood with which CMG passes increases in presence components known chromatid cohesion functions 7,10–14 or preventing from freely sliding along template DNA. retains topological entrapment passage, suggesting ring. The frequency further when fully reconstituted encounter rings, resulting successful establishment between products. Our findings demonstrate existence simple links chromosome segregation, rings.

Language: Английский

Citations

2