Efficient nepetalactone production in Saccharomyces cerevisiae via metabolic engineering and bioprocess optimization

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132440 - 132440

Published: March 1, 2025

Language: Английский

---

Plants as biofactories for production of the aphid sex pheromone nepetalactone

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Abstract Aphids cause massive agricultural losses through direct damage or as pathogen vectors. Control often relies on insecticides, which are expensive and not selective. An interesting alternative is to use aphid sex pheromones nepetalactone (NON) nepetalactol (NOL) interfere with mating attract predators. Here, we explore production of these compounds in plants, their precursors can be derived from mevalonate (MVA) methylerythritol phosphate (MEP) pathways. By introducing six genes, including a major latex protein‐like (MLPL) enzyme, engineered functional biosynthetic pathway into plants. Transient expression enzymes N. benthamiana caused nepetalactone, without the need for external supplementation substrates. Targeting all chloroplast appeared result higher NON yields than just chloroplast‐targeting first two enzymes. We could detect NOL, suggesting it rapidly oxidised NON. In addition, produced stably transformed Camelina sativa (Camelina) lines. Interestingly, specific enantiomer was different compared Camelina, indicating value platforms producing isoforms. This opens possibilities using plants green factories baits pheromone dispensers that insect behaviour.

Language: Английский

---

Current insights into plant volatile organic compound biosynthesis

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102708 - 102708

Published: March 28, 2025

Language: Английский

---