Glutamine Synthetase and Glutamate Synthase Family Perform Diverse Physiological Functions in Exogenous Hormones and Abiotic Stress Responses in Pyrus betulifolia Bunge (P.be) DOI Creative Commons

Weilong Zhang,

Shuai Yuan, Na Liu

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2759 - 2759

Published: Oct. 1, 2024

The unscientific application of nitrogen (N) fertilizer not only increases the economic input pear growers but also leads to environmental pollution. Improving plant N use efficiency (NUE) is most effective economical method solve above problems. absorption and utilization by plants a complicated process. Glutamine synthetase (GS) glutamate synthase (GOGAT) are crucial for synthesizing from ammonium in plants. However, their gene family pears has been documented. This study identified 29 genes belonging GS GOGAT genomes Pyrus betulaefolia (P.be, 10 genes), pyrifolia (P.py, 9 bretschneideri (P.br, genes). These were classified into two subgroups (GS1 GS2) (Fd–GOGAT NADH–GOGAT). similar exon–intron structures conserved motifs within each cluster suggest evolutionary conservation these genes. Meanwhile, segmental duplication driven expansion evolution families pear. tissue–specific expression dynamics PbeGS PbeGOGAT significant roles growth development. Cis–acting elements promoters development, hormonal responses, stress reactions. Furthermore, qRT–PCR analysis indicated that PbeGSs PbeGOGATs showed differential under exogenous hormones (GA3, IAA, SA, ABA) abiotic (NO3− salt stress). In which, PbeGS2.2 was up–regulated hormone treatment down–regulated stress. physiological experiments demonstrated GA3 IAA promoted GS, Fd–GOGAT, NADH–GOGAT enzyme activities, as well content. Correlation revealed positive relationship between PbeGS1.1, PbeGS2.2, PbeNADH–GOGATs, Therefore, PbeNADH–GOGATs could be key candidate improving NUE response. To best our knowledge, provides valuable biological information about first time establishes foundation molecular breeding aimed at developing high rootstocks.

Language: Английский

Regulation of photosynthetic characteristics carbon and nitrogen metabolism and growth of maize seedlings by graphene oxide coating DOI Creative Commons
Xu Zhang, Weidong Huang,

Deyong Kong

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

Maize seedlings in cold regions and high latitude often face abiotic stress. As a result, weak affect maize production, The commonly used seed coating agents production are mainly to prevent biological stress of pests diseases, have little effect on seedling vigor resistance. In this experiment, the combination graphene oxide (GO) agent can effectively diseases increase growth seedlings.The effects different concentrations composite GO photosynthetic carbon nitrogen metabolism activity were verified by pot experiments. It provides reference for application nanomaterials adjuvant technology improvement quality. Using as model plant, study evaluates, 22 g/L(T1), 44 g/L(T2), 66 g/L(T3), 88 g/L(T4), add respectively. Commercial control (Con). Under conditions thickened stem regulating nutrients absorption roots seedlings. leaf thickness vascular bundle area increased, efficiency leaves improved. Finally, it promoted leaves. Therefore, g/L is most suitable concentration experiment.

Language: Английский

Citations

1
Long-term nitrogen and phosphorus fertilization improved crop yield by influencing rhizosphere nitrogen transformation processes DOI
Chunxiao Wu,

Furong Wei,

Benshuai Yan

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 208, P. 105968 - 105968

Published: Feb. 18, 2025

Language: Английский

Citations

1
The crosstalk between nitrate signaling and other signaling molecules in Arabidopsis thaliana DOI Creative Commons
Jingjing Mao, Zhen Tian, Jinhao Sun

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 10, 2025

Nitrate signaling coordinates the expression of a broad range genes involved in nitrate uptake, transport, and assimilation, playing crucial role plant growth development. Notably, interacts extensively with various messenger molecules, including phytohormones, calcium ions (Ca 2+ ), reactive oxygen species (ROS), peptides, sucrose. This crosstalk amplifies optimizes nutrient coordinating developmental processes enhancing stress tolerance. Understanding interactions between these molecules offers valuable insights into improving crop use efficiency (NUE), resilience, agricultural sustainability. Using Arabidopsis thaliana as model, this review consolidates current knowledge on its interplay other pathways that regulate development adaptation. Finally, highlights potential genetic strategies for NUE, contributing to more sustainable practices.

Language: Английский

Citations

0
Auxin regulates leaf senescence and nitrogen assimilation to promote grain filling in maize (Zea mays L.) DOI
Na Jiang, Peng Wang, Zhiqi Yang

et al.

Field Crops Research, Journal Year: 2025, Volume and Issue: 323, P. 109787 - 109787

Published: Feb. 6, 2025

Language: Английский

Citations

0
Integrating Agronomic and Molecular Advancements to Enhance Nitrogen Use Efficiency (NUE) and Promote Sustainable Rice Production DOI Creative Commons
Uttam Bahadur Kunwar, Nazer Manzoor,

Jiancheng Wen

et al.

Nitrogen, Journal Year: 2025, Volume and Issue: 6(2), P. 34 - 34

Published: May 14, 2025

Rice is a major crop for half of the world’s population, and nitrogen (N) fertilizers play crucial role in its production. However, imbalanced N fertilizer uses traditional fertilization practices have led to low use efficiency (NUE), increased footprints, reduced rice yields farmers’ income. There are limited studies where integration both agronomic molecular advancements enhance NUE discussed, particularly developing countries. This review highlights novel strategies NUE, yields, profitability, while minimizing environmental impact. The include 4R Nutrient Stewardship framework, enhanced (EENFs), nano-fertilizers, biochar-based fertilizers, biological fixation, sensor-based management rice-growing mechanisms focus on uptake, assimilation, utilization, highlighting hormones, key genes, transcription factors (TFs), regulatory pathways. Moreover, we examine promising genotypes cultivars with improved grain yield. Additionally, this paper offers deep insights into recent genetics, such as multi-omics approaches (transcriptomics, metabolomics, metagenomics), Genome-Wide Association Study (GWAS), Quantitative Traits Loci mapping (QTLs), Single Nucleotide Polymorphisms (SNPs) analysis, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas9)-mediated genome editing, which serve valuable tools

Language: Английский

Citations

0
Glutamine Synthetase and Glutamate Synthase Family Perform Diverse Physiological Functions in Exogenous Hormones and Abiotic Stress Responses in Pyrus betulifolia Bunge (P.be) DOI Creative Commons

Weilong Zhang,

Shuai Yuan, Na Liu

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2759 - 2759

Published: Oct. 1, 2024

The unscientific application of nitrogen (N) fertilizer not only increases the economic input pear growers but also leads to environmental pollution. Improving plant N use efficiency (NUE) is most effective economical method solve above problems. absorption and utilization by plants a complicated process. Glutamine synthetase (GS) glutamate synthase (GOGAT) are crucial for synthesizing from ammonium in plants. However, their gene family pears has been documented. This study identified 29 genes belonging GS GOGAT genomes Pyrus betulaefolia (P.be, 10 genes), pyrifolia (P.py, 9 bretschneideri (P.br, genes). These were classified into two subgroups (GS1 GS2) (Fd–GOGAT NADH–GOGAT). similar exon–intron structures conserved motifs within each cluster suggest evolutionary conservation these genes. Meanwhile, segmental duplication driven expansion evolution families pear. tissue–specific expression dynamics PbeGS PbeGOGAT significant roles growth development. Cis–acting elements promoters development, hormonal responses, stress reactions. Furthermore, qRT–PCR analysis indicated that PbeGSs PbeGOGATs showed differential under exogenous hormones (GA3, IAA, SA, ABA) abiotic (NO3− salt stress). In which, PbeGS2.2 was up–regulated hormone treatment down–regulated stress. physiological experiments demonstrated GA3 IAA promoted GS, Fd–GOGAT, NADH–GOGAT enzyme activities, as well content. Correlation revealed positive relationship between PbeGS1.1, PbeGS2.2, PbeNADH–GOGATs, Therefore, PbeNADH–GOGATs could be key candidate improving NUE response. To best our knowledge, provides valuable biological information about first time establishes foundation molecular breeding aimed at developing high rootstocks.

Language: Английский

Citations

3