Establishment and biological characterization of radioresistant colorectal cancer cell lines DOI Creative Commons

Tianyin Qu,

Qing Dai, Jing Leng

et al.

FEBS Open Bio, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC). We established radiotherapy-resistant cell lines to explore the molecular mechanisms radiotherapy CRC. HT29 HCT116 cells were subjected repeated irradiation at 2 Gy establish these lines. CCK-8 assay, colony formation, xenograft tumor experiments used detect radiosensitivity cells. DNA damage repair proteins, GSH content, intracellular ROS also assayed parental resistant successfully HT29R HCT116R radioresistant after fractionated irradiation, showed significant tolerance further compared with stronger capacity for repair. Meanwhile, ionizing radiation significantly reduced but had no effect on content These results demonstrate that by method continuous Gy. This provides basis exploration mechanism resistance.

Language: Английский

Establishment and biological characterization of radioresistant colorectal cancer cell lines DOI Creative Commons

Tianyin Qu,

Qing Dai, Jing Leng

et al.

FEBS Open Bio, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC). We established radiotherapy-resistant cell lines to explore the molecular mechanisms radiotherapy CRC. HT29 HCT116 cells were subjected repeated irradiation at 2 Gy establish these lines. CCK-8 assay, colony formation, xenograft tumor experiments used detect radiosensitivity cells. DNA damage repair proteins, GSH content, intracellular ROS also assayed parental resistant successfully HT29R HCT116R radioresistant after fractionated irradiation, showed significant tolerance further compared with stronger capacity for repair. Meanwhile, ionizing radiation significantly reduced but had no effect on content These results demonstrate that by method continuous Gy. This provides basis exploration mechanism resistance.

Language: Английский

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