Development of a behavioral assay for testing essential oil repellency with Aedes aegypti mosquitoes DOI Creative Commons
Natalie L. Kendziorski, Tse‐Yu Chen,

Sara Farless

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 19, 2023

Abstract In the following methods article, an essential oil is implemented in a behavioral assay to function as spatial repellent. The purpose ascertain its effectiveness repelling vector mosquito, Aedes aegypti (Diptera: Culicidae) based on exposure volatiles, not contact. A modified dual-choice chamber system was developed test efficacy of presence attractant or activator (carbon dioxide). composed 3 glass chambers arranged linearly with 2 metal gates that connect chambers. can be opened release mosquitoes from central and then closed isolate responding into treatment control end Host-seeking responsive host stimuli were pre-selected for use assays reduce variability. allows rapid estimation repellency each only 10 minutes. Movement individual recorded by counting total number chamber. Repellency determined calculating repellent activity index guidelines WHO. choice detected aversive behavior thyme against three populations . Vero Pernambuco had higher indices compared Manatee population. This used any Additionally, this may properties other flying host-seeking insects besides mosquitoes. Data gathered these will contribute entomological knowledge cues responses repellents.

Language: Английский

A critical review of current laboratory methods used to evaluate mosquito repellents DOI Creative Commons
Hailey A. Luker

Frontiers in Insect Science, Journal Year: 2024, Volume and Issue: 4

Published: Jan. 18, 2024

Pathogens transmitted by mosquitoes threaten human health around the globe. The use of effective mosquito repellents can protect individuals from contracting mosquito-borne diseases. Collecting evidence to confirm and quantify effectiveness a repellent is crucial requires thorough standardized testing. There are multitudes methods test that each have their own strengths weaknesses. Determining which type conduct be challenging collection currently used has changed over time. Some these powerful rapidly screen numerous putative treatments. Other responses specific treatments measure either spatial or contact repellency. A subset uses live animals volunteers repellency Assays greatly vary in affordability accessibility for researchers and/or may require additional results. Here I present critical review covers some most frequently laboratory assays last two decades. discuss experimental designs highlight weaknesses method covered.

Language: Английский

Citations

5
Revealing complex mosquito behaviour: a review of current automated video tracking systems suitable for tracking mosquitoes in the field DOI Creative Commons

Beatrice H. Bredt,

Frédéric Tripet, Pie Müller

et al.

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
Development of an automated biomaterial platform to study mosquito feeding behavior DOI Creative Commons
Kevin D. Janson, Brendan H. Carter, Samuel B. Jameson

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 9, 2023

Mosquitoes carry a number of deadly pathogens that are transmitted while feeding on blood through the skin, and studying mosquito behavior could elucidate countermeasures to mitigate biting. Although this type research has existed for decades, there yet be compelling example controlled environment test impact multiple variables behavior. In study, we leveraged uniformly bioprinted vascularized skin mimics create platform with independently tunable sites. Our allows us observe collect video data 30-45 min. We maximized throughput by developing highly accurate computer vision model (mean average precision: 92.5%) automatically processes videos increases measurement objectivity. This enables assessment critical factors such as activity around sites, used it evaluate repellent effect DEET oil lemon eucalyptus-based repellents. validated both repellents effectively repel mosquitoes in laboratory settings (0% experimental groups, 13.8% control group, p < 0.0001), suggesting our platform's use screening assay future. The is scalable, compact, reduces dependence vertebrate hosts research.

Language: Английский

Citations

7
Development of a behavioral assay for testing essential oil repellency with Aedes aegypti mosquitoes DOI Creative Commons
Natalie L. Kendziorski, Tse‐Yu Chen,

Sara Farless

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 19, 2023

Abstract In the following methods article, an essential oil is implemented in a behavioral assay to function as spatial repellent. The purpose ascertain its effectiveness repelling vector mosquito, Aedes aegypti (Diptera: Culicidae) based on exposure volatiles, not contact. A modified dual-choice chamber system was developed test efficacy of presence attractant or activator (carbon dioxide). composed 3 glass chambers arranged linearly with 2 metal gates that connect chambers. can be opened release mosquitoes from central and then closed isolate responding into treatment control end Host-seeking responsive host stimuli were pre-selected for use assays reduce variability. allows rapid estimation repellency each only 10 minutes. Movement individual recorded by counting total number chamber. Repellency determined calculating repellent activity index guidelines WHO. choice detected aversive behavior thyme against three populations . Vero Pernambuco had higher indices compared Manatee population. This used any Additionally, this may properties other flying host-seeking insects besides mosquitoes. Data gathered these will contribute entomological knowledge cues responses repellents.

Language: Английский

Citations

0