Hooked on zombie worms? Genetic blueprints of bristle formation in Osedax japonicus (Annelida) DOI Creative Commons
Tilic Ekin, Norio Miyamoto, María Herranz

et al.

EvoDevo, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 4, 2024

Abstract Background This study sheds light on the genetic blueprints of chaetogenesis (bristle formation), a complex biomineralization process essential not only for diverse group bristle worms (annelids) but also other spiralians. We explore mechanisms behind chaetae formation in Osedax japonicus , bone-devouring deep-sea worm known its unique ecological niche and morphological adaptations. Results characterized chaetal structure musculature using electron microscopy immunohistochemistry, combined RNAseq larval stages with in-situ hybridization chain reaction (HCR) to reveal gene expression patterns integral chaetogenesis. Our findings pinpoint distinct surge during stage active chaetogenesis, identifying specific genes cells involved. Conclusions research underscores value studying non-model, "aberrant" organisms like whose unique, temporally restricted provided insights into elevated across led identification critical formation. The identified as directly involved lay groundwork future comparative studies Annelida Spiralia, potentially elucidating homology chaetae-like chitinous structures their evolution.

Language: Английский

A Cambrian spiny stem mollusk and the deep homology of lophotrochozoan scleritomes DOI
Guangxu Zhang, Luke A. Parry, Jakob Vinther

et al.

Science, Journal Year: 2024, Volume and Issue: 385(6708), P. 528 - 532

Published: Aug. 1, 2024

Mollusks encompass enormous disparity, including familiar clams and snails alongside less aculiferans (chitons vermiform aplacophorans) with complex multicomponent skeletons. Paleozoic fossils trace crown mollusks to forms exhibiting a combination of biomineralized shells sclerites (e.g., scales, spines, spicules). We describe shell-less, Cambrian stem mollusk, Shishania aculeata gen. et sp. nov., conical, hollow chitinous smooth girdle, broad foot, mantle cavity. The have microstructure narrow canals consistent the impressions chaetal microvilli found in annelids brachiopods. provide morphological stepping stone between typical chaetae (chitinous bristles) external organic part aculiferan that encloses mineralized body. This discovery reinforces common origin lophotrochozoan sclerites, suggesting mollusk ancestor was densely covered chaetae.

Language: Английский

Citations

6
Discovery and characterization of a transient chaetal gland during the development of Capitella teleta (Sedentaria: Annelida) DOI Creative Commons
Tilic Ekin, Thomas Bartolomaeus, Elaine C. Seaver

et al.

Journal of Morphology, Journal Year: 2024, Volume and Issue: 285(6)

Published: June 1, 2024

Abstract Chaetae are among the most extensively studied structures in polychaetes, serving as a defining morphological trait for annelids. Capitella teleta stands out one of few established annelid models developmental and studies, thus receiving significant scholarly attention. In this study, we unveil previously unnoticed glandular structure associated with chaetae within larvae C. . Our investigations demonstrate absence comparable chaetal follicles adults juveniles (older than 1 week), well during active chaetogenesis, underscoring transient nature these glands. This indicates that larval transform into gland later disappears. Utilizing histology transmission electron microscopy, characterized findings underscore diversity ultrastructure annelids show that, even well‐studied species, novel details can be found. We emphasize importance examining various life‐history stages to capture such features. work lays crucial foundation deepens our understanding chaetogenesis , paving way more accurate interpretations future experimental studies on species.

Language: Английский

Citations

1
Sex-biased gene expression precedes sexual dimorphism in the agonadal annelidPlatynereis dumerilii DOI Creative Commons
Rannyele Passos Ribeiro, Ryan W. Null, B. Duygu Özpolat

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Gametogenesis is the process by which germ cells differentiate into mature sperm and oocytes, essential for sexual reproduction. The sex-specific molecular programs that drive spermatogenesis oogenesis can also serve as sex identification markers. Platynereis dumerilii a research organism has been studied in many areas of developmental biology. However investigations often disregard sex, P. juveniles lack dimorphism. mechanisms gametogenesis segmented worm are largely unknown. In this study, we used RNA sequencing to investigate transcriptomic profiles juveniles. Our analysis revealed sex-biased gene expression becomes increasingly pronounced during advanced stages, particularly meiotic phases gametogenesis. We identified conserved genes associated with spermatogenesis, such dmrt1, novel psmt, oogenesis. Additionally, putative long non-coding RNAs were upregulated both male female gametogenic programs. This study provides foundational resource cell dumerilii, markers identification, offers comparative data enhance our understanding evolution across species.

Language: Английский

Citations

1
Antero-posterior gradients of cell plasticity and proliferation modulate posterior regeneration in the annelidPlatynereis DOI Creative Commons
Loïc Bideau,

Loeiza Baduel,

Marianne Basso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 12, 2023

Abstract Regenerative abilities are extremely variable among animals and may be substantial in some phyla, such as the annelids. So far, cellular mechanisms underlying regeneration annelids remain elusive. To precisely determine origin(s), plasticity fate of cells participating blastema formation during posterior annelid Platynereis dumerilii , we developed specific tools to track proliferative well gut epithelial cells. We showed that two populations progenitors at play that, them, from differentiated tissues lineage-restricted. Strikingly, less more much plastic can produce ectodermal mesodermal derivatives, addition However, their is de facto limited exemplified by inability regenerate stem responsible for constant growth worms. evidenced those local origin ( i.e. segment abutting amputation plan) most Our results favour a hybrid flexible model relying on gradient cell along antero-posterior axis animal.

Language: Английский

Citations

2
Dynamic microvilli sculpt bristles at nanometric scale DOI Creative Commons
Kyojiro N. Ikeda, Ilya Belevich, Luis Zelaya-Lainez

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 13, 2024

Abstract Organisms generate shapes across size scales. Whereas patterning and morphogenesis of macroscopic tissues has been extensively studied, the principles underlying formation micrometric submicrometric structures remain largely enigmatic. Individual cells polychaete annelids, so-called chaetoblasts, are associated with generation chitinous bristles highly stereotypic geometry. Here we show that bristle requires a chitin-producing enzyme specifically expressed in chaetoblasts. Chaetoblasts exhibit dynamic cell surfaces stereotypical patterns actin-rich microvilli. These microvilli can be matched internal external reconstructed from serial block-face electron micrographs. chitin teeth deposited by an extension-disassembly cycle resembling biological 3D printer. Consistently, pharmacological interference actin dynamics leads to defects tooth formation. Our study reveals both material shape encoded same cell, play role micro- sculpting biomaterials.

Language: Английский

Citations

0
Hooked on zombie worms? Genetic blueprints of bristle formation in Osedax japonicus (Annelida) DOI Creative Commons
Tilic Ekin, Norio Miyamoto, María Herranz

et al.

EvoDevo, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 4, 2024

Abstract Background This study sheds light on the genetic blueprints of chaetogenesis (bristle formation), a complex biomineralization process essential not only for diverse group bristle worms (annelids) but also other spiralians. We explore mechanisms behind chaetae formation in Osedax japonicus , bone-devouring deep-sea worm known its unique ecological niche and morphological adaptations. Results characterized chaetal structure musculature using electron microscopy immunohistochemistry, combined RNAseq larval stages with in-situ hybridization chain reaction (HCR) to reveal gene expression patterns integral chaetogenesis. Our findings pinpoint distinct surge during stage active chaetogenesis, identifying specific genes cells involved. Conclusions research underscores value studying non-model, "aberrant" organisms like whose unique, temporally restricted provided insights into elevated across led identification critical formation. The identified as directly involved lay groundwork future comparative studies Annelida Spiralia, potentially elucidating homology chaetae-like chitinous structures their evolution.

Language: Английский

Citations

0