Template-Assisted Assembly of Hybrid DNA/RNA Nanostructures Using Branched Oligodeoxy- and Oligoribonucleotides DOI Open Access
А. А. Фокина,

Yulia Poletaeva,

Svetlana Dukova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15978 - 15978

Published: Nov. 5, 2023

A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The employed supramolecular oligonucleotide dendrimer as 3D template that was obtained via the hybridization of siRNA strands and single-stranded joined three- or four-way branched junctions. building block (a starlet) designed for composed three identical self-complementary single RNA strand oligonucleotides template. To prevent premature auto-hybridization in starlet, photolabile protecting group introduced N3-substituted thymidine phosphoramidite. Cleavable linkers such disulfide linkage, RNase sensitive triribonucleotides, di- trideoxynucleotides were incorporated into starlet at specific points guide post-assembly disconnection from template, enzymatic disassembly biological media. At same time, modified with 2′-OMe ribonucleotides phosphorothioate groups certain positions stabilize toward digestion. We report herein solid-phase synthesis oligodeoxy oligoribonucleotide blocks DNA/RNA dendritic construction after initial molecular modeling, followed by around DNA-coated visualization resulting nanostructure transmission electron microscopy.

Language: Английский

Employing toehold-mediated DNA strand displacement reactions for biomedical applications DOI Creative Commons

Keziah Jacob Souza,

Deepak K. Agrawal

Med-X, Journal Year: 2024, Volume and Issue: 2(1)

Published: Jan. 29, 2024

Abstract Dynamic DNA nanotechnology belongs to a larger umbrella of that primarily uses as nanoscopic material build mobile structures and cascaded reaction networks powered by oligonucleotides. A widely used mechanism construct dynamic system is toehold-mediated strand displacement reactions (TMSDRs). TMSDRs are easy engineer because the known base-pairing rules follow Watson–Crick model DNA, sequence-dependent binding rates, energies DNAs, whose secondary structure predictable. Due these attributes, have been develop enzyme-free isothermal with remarkable applications in diagnostics, therapeutics computing. In this review, we briefly introduce working principle TMSDRs, silico design considerations, diverse input output signals can be processed through TMSDRs. We then summarize recent where successfully employed detecting clinically relevant targets such single nucleotide polymorphisms variants, microRNAs whole cells programmable drug delivery vehicles regulation therapies including transcriptional protein regulations. also discuss driven biomedical hydrogels Finally, challenges each prospects engineering.

Language: Английский

Citations

3
Advances in micro-/nanorobots for cancer diagnosis and treatment: propulsion mechanisms, early detection, and cancer therapy DOI Creative Commons
Baiyang Fu, Dan Luo, Chao Li

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 6, 2025

In recent years, medical micro-/nanorobots (MNRs) have emerged as a promising technology for diagnosing and treating malignant tumors. MNRs enable precise, targeted actions at the cellular level, addressing several limitations of conventional cancer diagnosis treatment, such insufficient early diagnosis, nonspecific drug delivery, chemoresistance. This review provides an in-depth discussion propulsion mechanisms MNRs, including chemical fuels, external fields (light, ultrasound, magnetism), biological propulsion, hybrid methods, highlighting their respective advantages limitations. Additionally, we discuss novel approaches tumor precision surgery, emphasizing potential clinical applications. Despite significant advancements, challenges biocompatibility, efficiency, translation persist. examines current state MNR applications outlines future directions development, with aim enhancing diagnostic therapeutic efficacy facilitating integration into practice.

Language: Английский

Citations

0
Ferroptosis meets biomimetic nano-systems: A novel strategy for targeted cancer therapy DOI Creative Commons

M.Q. Zhang,

Jin Lan, Zibo Li

et al.

Published: May 1, 2025

Language: Английский

Citations

0
Therapeutic applications of nanobots and nanocarriers in cancer treatment DOI Open Access

Zarlish Khan,

Noor Khan, Mithra Geetha

et al.

Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: June 4, 2025

Language: Английский

Citations

0
Study of photodegradation performance of Zn‐doped CeO2 nanoparticles for wastewater contaminants remediation under visible light DOI
Khadijeh Hamidian, Mina Sarani, Shahriar Mohebbi

et al.

Applied Organometallic Chemistry, Journal Year: 2023, Volume and Issue: 37(7)

Published: April 25, 2023

The presented paper introduces a green route for the arrangement of pure and 1%, 2%, 4% zinc doped cerium oxide nanoparticles (Zn‐doped CeO 2 NPs) by involving extract Salvadora persica . analytical results powder X‐ray diffraction (PXRD), ultraviolet–visible (UV–Vis), field emission scanning electron microscopy (FESEM), energy dispersive (EDX), Raman techniques confirmed successful synthesis NPs. According to PXRD outcomes, NPs have cubic phase. Moreover, as result doping within crystal framework this material, noticeable alteration was observed in placement (101) peak. size measured FESEM analysis be around 20–25 nm, which decreased with into structure. UV–Vis appeared peak 343 cm −1 formation NPs; shifted zinc. active band F 2g at 459 graph can related fluorite type construction. we evaluated photocatalytic functionality prepared samples towards acid orange 7 (AO7) dye visible light. gathered data indicated 2% Zn‐doped optimum sample due eliminating 83% AO7 volume 15 mg L pH = 7. Therefore, performing process on these suitable choice improving activity dye.

Language: Английский

Citations

7
Recent advances in metal-organic frameworks synthesis and characterization with a focus on electrochemical determination of biological and food compounds, and investigation of their antibacterial performance DOI Creative Commons

Mehdi Khalaf Mohammad MAHJOOB,

Mahshid Akbarizadeh,

Paria HASHEMAN

et al.

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Journal Year: 2023, Volume and Issue: 51(8), P. 100286 - 100286

Published: May 27, 2023

Metal-organic frameworks (MOFs) are hybrid materials composed of both organic and inorganic elements. MOF materials, because their unique structures properties, such as controllable pore size, large surface area, excellent catalytic activity, high density active sites, applied in electrochemical sensors systems for the sensitive, quick, low-cost detection multiple analytes. MOFs, with specific shape, adjustable volume, will provide strong interaction compounds via abundant functional groups, leading to increased selectivity determination a wide range compounds. In this review, structure, synthesis methods, characterization, applications MOFs recent years sensing material fabricating detecting drugs, pesticides, heavy metals, food additives, other contaminants summarized, compared, discussed. Also, were used numerous antibacterial application areas due long-term release capability, stretch ability when integrated and/or substances (including nanostructures, phytochemicals, antibiotics, polymers). This review also provides comprehensive overview uses one's composite focus on different kinds nanocomposite influence various uses.

Language: Английский

Citations

7
Trace elements‐based Auroshell gold@hematite nanostructure: Green synthesis and their hyperthermia therapy DOI Creative Commons
Hadil Alahdal,

Sumya Ayad Abdullrezzaq,

Hawraz Ibrahim M. Amin

et al.

IET Nanobiotechnology, Journal Year: 2022, Volume and Issue: 17(1), P. 22 - 31

Published: Nov. 24, 2022

Hyperthermia is an additional treatment method to radiation therapy/chemotherapy, which increases the survival rate of patients without side effects. Nowadays, Auroshell nanoparticles have attracted much attention due their precise control over heat use for medical purposes. In this research, iron/gold were synthesised using green nanotechnology approach. gold@hematite and characterised with rosemary extract in one step by X-ray powder diffraction, SEM, high-resolution transmission electron microscopy, photoelectron spectroscopy analysis. Cytotoxicity iron@gold against normal HUVEC cells glioblastoma cancer was evaluated 2,5-diphenyl-2H-tetrazolium bromide method, water bath hyperthermia, combined hyperthermia nano-therapy. minimal toxicity are safe cells. The gold shell around magnetic core magnetite caused environmental cellular biocompatibility these nanoparticles. These targeted transfer tumour tissue led uniform heating malignant tumours as most efficient therapeutic agent.

Language: Английский

Citations

9
Mapping Temporally Ordered Inputs to Binary Message Outputs with a DNA Temporal Logic Circuit DOI Creative Commons
Shuai Zhao, Yuan Liu, Xiaokang Zhang

et al.

Nanomaterials, Journal Year: 2023, Volume and Issue: 13(5), P. 903 - 903

Published: Feb. 27, 2023

Molecular circuits and devices with temporal signal processing capability are of great significance for the analysis complex biological processes. Mapping inputs to binary messages is a process history-dependent responses, which can help understand signal-processing behavior organisms. Here, we propose DNA logic circuit based on strand displacement reactions, map temporally ordered corresponding message outputs. The presence or absence output determined by type substrate reaction input so that different orders correspond We demonstrate be generalized more increasing decreasing number substrates inputs. also show our had excellent responsiveness inputs, flexibility, expansibility in case symmetrically encrypted communications. envision scheme provide some new ideas future molecular encryption, information processing, neural networks.

Language: Английский

Citations

3
Template-Assisted Assembly of Hybrid DNA/RNA Nanostructures Using Branched Oligodeoxy- and Oligoribonucleotides DOI Open Access
А. А. Фокина,

Yulia Poletaeva,

Svetlana Dukova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15978 - 15978

Published: Nov. 5, 2023

A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The employed supramolecular oligonucleotide dendrimer as 3D template that was obtained via the hybridization of siRNA strands and single-stranded joined three- or four-way branched junctions. building block (a starlet) designed for composed three identical self-complementary single RNA strand oligonucleotides template. To prevent premature auto-hybridization in starlet, photolabile protecting group introduced N3-substituted thymidine phosphoramidite. Cleavable linkers such disulfide linkage, RNase sensitive triribonucleotides, di- trideoxynucleotides were incorporated into starlet at specific points guide post-assembly disconnection from template, enzymatic disassembly biological media. At same time, modified with 2′-OMe ribonucleotides phosphorothioate groups certain positions stabilize toward digestion. We report herein solid-phase synthesis oligodeoxy oligoribonucleotide blocks DNA/RNA dendritic construction after initial molecular modeling, followed by around DNA-coated visualization resulting nanostructure transmission electron microscopy.

Language: Английский

Citations

0