Stability of a Mutualistic Escherichia coli Co‐Culture During Violacein Production Depends on the Kind of Carbon Source DOI Creative Commons

Simon Schick,

Tobias Müller, Ralf Takors

et al.

Engineering in Life Sciences, Journal Year: 2024, Volume and Issue: 24(10)

Published: Sept. 8, 2024

ABSTRACT The L‐tryptophan–derived purple pigment violacein (VIO) is produced in recombinant bacteria and studied for its versatile applications. Microbial synthetic co‐cultures are gaining more importance as efficient factories synthesizing high‐value compounds. In this work, a mutualistic cross‐feeding Escherichia coli co‐culture metabolically engineered to produce VIO. strains genetically modified by auxotrophies the tryptophan (TRP) pathway enable metabolic division of labor. Therein, one strain produces anthranilate (ANT) other transforms it into TRP further Population dynamics stability depend on choice carbon source, impacting presence thus exchange metabolites well overall VIO productivity. Four sources (D‐glucose, glycerol, D‐galactose, D‐xylose) were compared. D‐Xylose led which showed stable growth production, ANT‐TRP exchange, enhanced production. Best titers ∼126 mg L –1 shake flasks. study demonstrates advantages approach synthesis highlights source's role Transferring knowledge an up‐scaled bioreactor system has great potential improving

Language: Английский

Harnessing microbial co-culture to increase the production of known secondary metabolites DOI
Yuzhen Li, Wanqi Zhang, Pengfei Hu

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Co-culturing may provoke the biosynthesis of novel natural products. However, various microbial co-culture techniques also boost fermentation titer, yield and rate known products – we review current progress towards such a goal.

Language: Английский

Citations

1
Stability of a Mutualistic Escherichia coli Co‐Culture During Violacein Production Depends on the Kind of Carbon Source DOI Creative Commons

Simon Schick,

Tobias Müller, Ralf Takors

et al.

Engineering in Life Sciences, Journal Year: 2024, Volume and Issue: 24(10)

Published: Sept. 8, 2024

ABSTRACT The L‐tryptophan–derived purple pigment violacein (VIO) is produced in recombinant bacteria and studied for its versatile applications. Microbial synthetic co‐cultures are gaining more importance as efficient factories synthesizing high‐value compounds. In this work, a mutualistic cross‐feeding Escherichia coli co‐culture metabolically engineered to produce VIO. strains genetically modified by auxotrophies the tryptophan (TRP) pathway enable metabolic division of labor. Therein, one strain produces anthranilate (ANT) other transforms it into TRP further Population dynamics stability depend on choice carbon source, impacting presence thus exchange metabolites well overall VIO productivity. Four sources (D‐glucose, glycerol, D‐galactose, D‐xylose) were compared. D‐Xylose led which showed stable growth production, ANT‐TRP exchange, enhanced production. Best titers ∼126 mg L –1 shake flasks. study demonstrates advantages approach synthesis highlights source's role Transferring knowledge an up‐scaled bioreactor system has great potential improving

Language: Английский

Citations

1