Identification of m6 A-regulated ferroptosis biomarkers for prognosis in laryngeal cancer DOI Creative Commons
Xin Wang, Wen Zhang, Kun Liang

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 14, 2025

Laryngeal cancer (LC) is a malignant tumor that occurs in the larynx. N6-methyladenosine (m6A) RNA methylation, pivotal and prevalent epigenetic modification eukaryotic mRNA, intricately intertwines with ferroptosis, together, they play crucial role development of LC. Accordingly, further research on related molecular mechanisms pathology LC necessary. Weighted gene co-expression network analysis correlation were used to identify differentially expressed m6A-related ferroptosis genes The TCGA-HNSC GSE65858 datasets obtained from public databases. dataset consisted 110 primary oropharynx samples 12 control samples, while contained forty-eight samples. Univariate Cox least absolute shrinkage selection operator (LASSO) regression utilized for feature risk model construction dataset. was validated Then, nomogram built based independent prognostic factor identified using univariate multivariate Mutation analysis, immune-related drug sensitivity prediction applied analyze utility Additionally, qRT-PCR western blot performed detect TFRC, RGS4, FTH1 expression. Three biomarkers build LASSO algorithms. Receiver operating characteristic (ROC) verified accuracy model. Tumor Immune Dysfunction Exclusion (TIDE) Estimation STromal cells MAlignant Tumors Expression data (ESTIMATE) algorithm showed positive relationship between score TIDE or ESTIMATE score. Furthermore, found 19 chemotherapy drugs strongly correlated exhibited high expression levels 30 laryngeal carcinoma tissues cell lines. Notably, TFRC significantly associated patient prognosis. In Conclusion, FTH1, as m6A-regulated LC, providing insights into treatment

Language: Английский

Identification of m6 A-regulated ferroptosis biomarkers for prognosis in laryngeal cancer DOI Creative Commons
Xin Wang, Wen Zhang, Kun Liang

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 14, 2025

Laryngeal cancer (LC) is a malignant tumor that occurs in the larynx. N6-methyladenosine (m6A) RNA methylation, pivotal and prevalent epigenetic modification eukaryotic mRNA, intricately intertwines with ferroptosis, together, they play crucial role development of LC. Accordingly, further research on related molecular mechanisms pathology LC necessary. Weighted gene co-expression network analysis correlation were used to identify differentially expressed m6A-related ferroptosis genes The TCGA-HNSC GSE65858 datasets obtained from public databases. dataset consisted 110 primary oropharynx samples 12 control samples, while contained forty-eight samples. Univariate Cox least absolute shrinkage selection operator (LASSO) regression utilized for feature risk model construction dataset. was validated Then, nomogram built based independent prognostic factor identified using univariate multivariate Mutation analysis, immune-related drug sensitivity prediction applied analyze utility Additionally, qRT-PCR western blot performed detect TFRC, RGS4, FTH1 expression. Three biomarkers build LASSO algorithms. Receiver operating characteristic (ROC) verified accuracy model. Tumor Immune Dysfunction Exclusion (TIDE) Estimation STromal cells MAlignant Tumors Expression data (ESTIMATE) algorithm showed positive relationship between score TIDE or ESTIMATE score. Furthermore, found 19 chemotherapy drugs strongly correlated exhibited high expression levels 30 laryngeal carcinoma tissues cell lines. Notably, TFRC significantly associated patient prognosis. In Conclusion, FTH1, as m6A-regulated LC, providing insights into treatment

Language: Английский

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