Microchimica Acta, Journal Year: 2023, Volume and Issue: 190(8)
Published: July 25, 2023
Language: Английский
Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 467, P. 143513 - 143513
Published: May 17, 2023
Recycled additive manufacturing sensing platforms are fabricated with carboxylated multi-walled carbon nanotubes (COOH-MWCNT) exhibit enhanced electrochemical biosensor performance allowing for the direct coupling of biorecognition element to COOH-MWCNT preparation an genosensor detection yellow fever virus cDNA. Bespoke filaments was produced using recycled poly(lactic acid) (rPLA, 65 wt%), polyethylene succinate (PES, 10 black (CB, 15 and (10 wt%) which exhibits over that commercial filament. A bespoke all-in-one manufactured electroanalytical cell is proposed, working, reference counter electrodes in addition a modification rim allows facile production biosensors through application droplets. The applied Virus cDNA anodic square wave voltammetry; linear dynamic range (LDR) 0.5–15 µM R2 0.9995, sensitivity 177 ± 2 µA µM−1, limit (LOD) 0.138 µM, quantification (LOQ) 0.859 were obtained. This work highlights how filament can enhance biosensing platforms, whilst feedstock improve end-product sustainability.
Language: Английский
Citations
45
ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(7), P. 2591 - 2613
Published: June 15, 2023
Cardiovascular disease is a global health threat, and detecting cardiac biomarkers essential for early-stage diagnosis personalized treatment. Traditional approaches have limitations, but optical nanobiosensors offer rapid, highly selective, sensitive detection. Optical generate biosignals that transfer light signals while analytes bind with the bioreceptors. advantages such as ease of monitoring, low cost, wide detection range, high sensitivity without any interference. An nanobiosensor platform promising approach point-of-care biomarker limit. This review mainly focuses on cardiovascular based various been reported during last five years, we categorized them signal readouts. A detailed discussion classification biomarkers, design strategies biosensors, types optically active nanomaterials, bioreceptors, functionalization techniques, assay types, sensing mechanisms presented. Then, summarize signaling readout-based systems biomarkers. Finally, conclude recent development testing (PoCT) used in readout techniques.
Language: Английский
Citations
32
ACS Sensors, Journal Year: 2023, Volume and Issue: 8(8), P. 2898 - 2920
Published: Aug. 9, 2023
Over the past decade, molecular imprinting (MI) technology has made tremendous progress, and advancements in nanotechnology have been major driving force behind improvement of MI technology. The preparation nanoscale imprinted materials, i.e., molecularly polymer nanoparticles (MIP NPs, also commonly called nanoMIPs), opened new horizons terms practical applications, including field sensors. Currently, hydrogels are very promising for applications bioanalytical assays sensors due to their high biocompatibility possibility tune chemical composition, size (microgels, nanogels, etc.), format (nanostructures, MIP film, fibers, etc.) prepare optimized analyte-responsive materials. This review aims highlight recent progress on use hydrogel NPs biosensing purposes over mainly focusing incorporation sensing devices detection a fundamental class biomolecules, peptides proteins. begins by directing its focus ability MIPs replace biological antibodies (bio)analytical great potential face current demands several fields, such as disease diagnosis, food safety, environmental monitoring, among others. After that, we address general advantages nanosized macro/micro-MIP higher affinity toward target analytes improved binding kinetics. Then, provide overview properties Sensors, followed brief description popular routes synthesis nanospheres targeting large namely precipitation polymerization solid-phase synthesis, along with fruitful combination epitope reliable approaches developing protein-imprinted In second part review, provided state art application nanogels screening macromolecules having different transduction modes (optical, electrochemical, thermal, design formats single use, reusable, continuous even multiple analyte specialized laboratories or situ using mobile Finally, explore aspects about development this discuss areas future growth.
Language: Английский
Citations
30
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 165, P. 117145 - 117145
Published: June 18, 2023
Language: Английский
Citations
25
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 255, P. 116090 - 116090
Published: Feb. 5, 2024
Language: Английский
Citations
12
Biosensors and Bioelectronics X, Journal Year: 2022, Volume and Issue: 13, P. 100288 - 100288
Published: Dec. 15, 2022
IL-6 is a pleiotropic cytokine that exerts pathological proinflammatory effects in various acute, chronic, and communicable diseases. These diseases place significant burden on individuals, healthcare, economic systems; thus, rapid low-cost diagnosis prognosis sensor systems are urgently needed. In this review, we critically evaluate electrochemical sensors for detecting different biofluids. First, investigate the roles of structure, binding, pathways pathologies observe detectable levels present Second, discuss functionalization sensing approaches using (1) antibodies, (2) aptamers, (3) receptors, (4) molecularly imprinted polymers selective detection with practical wide linear ranges. Third, explain unexpected results caused by possible interactions between components, sample environment, may provide an excellent scope future research. Finally, believe fabricating reliable non-invasive exceptional efficacy will facilitate development portable diagnostic monitoring accelerate speed worldwide.
Language: Английский
Citations
37
Biomedicines, Journal Year: 2022, Volume and Issue: 10(5), P. 1085 - 1085
Published: May 6, 2022
The detection of cardiac biomarkers is used for diagnostics, prognostics, and the risk assessment cardiovascular diseases. analysis routinely performed with high-sensitivity immunological assays. Aptamers offer an attractive alternative to antibodies analytical applications but, date, are not widely practically implemented in diagnostics medicinal research. This review summarizes information on most common current state aptamer research regarding these biomarkers. as tool well established troponin I, T, myoglobin, C-reactive protein. For rest considered biomarkers, isolation novel aptamers or more detailed characterization known required. More attention should be addressed development dual-aptamer sandwich assays studies sensing biological fluids. universalization aptamer-based biomarker platforms integration clinical demanded practical implementation routine diagnostics. Nevertheless, wide usage diseases promising future, respect both point-of-care laboratory testing.
Language: Английский
Citations
34
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115220 - 115220
Published: March 11, 2023
This manuscript presents the design and facile production of screen-printed arrays (SPAs) for internally validated determination raised levels serum procalcitonin (PCT). The screen-printing methodology produced SPAs with six individual working electrodes that exhibit an inter-array reproducibility 3.64% 5.51% electrochemically active surface area heterogenous electrochemical rate constant respectively. were modified antibodies specific detection PCT through a methodology, where each stage simply uses droplets incubated on surface, allowing their mass-production. platform was used PCT, achieving linear dynamic range between 1 10 ng mL-1 sensor sensitivity 1.35 × 10-10 NIC%/ng mL-1. SPA intra- inter-day %RSD 4.00 5.05%, material cost £1.14. Internally human results (3 sample measurements, 3 control) (>2 mL-1) obtained, no interference effects seen from CRP IL-6. has potential to offer clinicians vital information rapidly begin treatment "query sepsis" patients while awaiting more lengthy remote laboratory testing methods. Analytical ranges tested make this ideal approach rapid in patient populations (such as neonates or critically ill patients) which are inherently wider. Due modification methods, we predict could be various analytes single array, array increased further maintain internal validation system.
Language: Английский
Citations
21
Microchemical Journal, Journal Year: 2023, Volume and Issue: 193, P. 109063 - 109063
Published: July 8, 2023
Language: Английский
Citations
19
Biosensors, Journal Year: 2023, Volume and Issue: 13(6), P. 632 - 632
Published: June 7, 2023
Biomarkers play key roles in the diagnosis, risk assessment, treatment and supervision of cardiovascular diseases (CVD). Optical biosensors assays are valuable analytical tools answering need for fast reliable measurements biomarker levels. This review presents a survey recent literature with focus on past 5 years. The data indicate continuing trends towards multiplexed, simpler, cheaper, faster innovative sensing while newer tendencies concern minimizing sample volume or using alternative sampling matrices such as saliva less invasive assays. Utilizing enzyme-mimicking activity nanomaterials gained ground comparison to their more traditional signaling probes, immobilization supports biomolecules signal amplification. growing use aptamers replacements antibodies prompted emerging applications DNA amplification editing techniques. were tested larger sets clinical samples compared current standard methods. ambitious goals horizon CVD testing include discovery determination relevant biomarkers help artificial intelligence, stable specific recognition elements fast, cheap readers disposable tests facilitate rapid at home. As field is progressing an impressive pace, opportunities optical remain significant.
Language: Английский
Citations
18