Novel Multimode Assay Based on Asymmetrically Competitive CRISPR and Raman Barcode Spectra for Multiple Hepatocellular Carcinoma Biomarkers Detection

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Commercial pregnancy test strips (PTS) possess the advantages of lower price, higher stability, and better repeatability have been popularized to integrate with novel sensing strategies detect other disease biomarkers, which accelerates commercialization process those strategies. However, current integration into commercial PTS still faced problems insufficient quantification, low sensitivity, lack multiple detection capabilities. Hence, we proposed concept "visual classification recognition, spectral signal subdivision" for hepatocellular carcinoma biomarkers (miRNA122 miRNA233) dual signals based on asymmetric competitive CRISPR (acCRISPR) surface-enhanced Raman spectroscopy coupling PTS, named acCRISPR-PTS-SERS assay. In this assay, acCRISPR was used as a nonamplified cascaded amplification method improve sensitivity detection. Two AuNPs-based core-shell tags, each corresponding different miRNA were achieve both visual recognition segmentation enhance quantification capability Under optimal conditions, LOD miRNA122 miRNA223 10.36 4.65 fM, respectively. The enhanced by nearly 2 orders magnitude. future, simultaneous hand-held fingerprint barcodes cancers can be achieved assistance microfluidic chip smartphone.

Language: Английский

Microfluidic detection using G-quadruplex DNA-based biosensors

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118121 - 118121

Published: Dec. 1, 2024

Language: Английский