Sensitive Detection of 17β-Estradiol in Aqueous Medium via a Ternary Emission Molecularly Imprinted Sensor: A Comprehensive Scientific Research Experiment for Undergraduates DOI
Yu Qiao, Na Wang, Liju Tan

et al.

Journal of Chemical Education, Journal Year: 2024, Volume and Issue: 102(1), P. 27 - 33

Published: Dec. 10, 2024

This study designed a comprehensive short laboratory course for senior undergraduate students to detect type of estradiol (17β-estradiol, E2) in an aqueous medium using ternary emission molecularly imprinted sensor. E2 is the most potent form estrogen, and monitoring its residue levels food environment crucial ensuring safety. Senior majoring chemistry or related areas often need more practical experience deeper understanding scientific research methodologies. To address this issue, employs molecular imprinting technology quantum dot develop polymer (MIP) fluorescence sensors real-time detection trace E2. The includes literature review, sample pretreatment, synthesis, material preparation, characterization, as well data processing analysis. These courses provide with complete facilitate transition postgraduate-level work.

Language: Английский

FRET-Based Dual-Color Carbon Dot Ratiometric Fluorescent Sensor Enables the Smartphone-Integrated Device for Noninvasive and Portable Diagnosis of Chronic Kidney Disease DOI
Mengyu Sun,

Maosheng Liang,

Rongmei Kong

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(44), P. 17907 - 17913

Published: Oct. 24, 2024

Cystatin C (Cys C), a crucial renal disease marker for chronic kidney (CKD), plays vital role in early diagnosis and treatment guidance. However, most current methods detecting Cys rely on single signal find it difficult to perform noninvasive portable diagnosis. Here, we developed ratiometric fluorescent carbon dot (CD) detection system point-of-care testing (POCT) of through fluorescence resonance energy transfer (FRET). The is based the hydrolysis effect papain bovine serum albumin (BSA) scaffold specific inhibitory papain, endowing high-resolution color variance. Moreover, low-cost, portable, yet reliable smartphone-assisted miniaturized device real-time quantitative POCT has been with limit (LOD) as low 0.4 μg/mL. This sensing platform can effectively differentiate patients from healthy volunteers, which facilitates self-screening individuals home monitoring CKD patients.

Language: Английский

Citations

5
Ternary-Emission Molecularly Imprinted Ratiometric Fluorescence Sensor and Kit for the Rapid and Visual Detection of Enrofloxacin DOI Creative Commons
Shijie Liu,

Jing‐Yi Yan,

Dani Sun

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 226 - 226

Published: April 2, 2025

In this study, a RGB based ternary-emission molecularly imprinted ratiometric fluorescence (MI-RFL) sensor was facilely constructed by using post-imprinting mixing strategy for the sensitive detection of enrofloxacin (ENR). Upon excitation at 365 nm, MI-RFL exhibited significant emission peaks 450, 550, and 620 nm. As ENR concentration increased, blue generated in sensing system gradually intensified, while red green emitted quantum dots polymers (MIPs) significantly quenched. Sensing conditions were systematically investigated, including wavelength, ratio red/green MIPs, pH buffer solution, reaction time. Under optimal conditions, developed showed good linear relationship within range 0.25–4 ppm along with obvious color change, low limit 0.134 ppm. High selectivity also attained an imprinting factor up to 11.65. When applied real samples seawater seafood, recovery rates 94.3–126.4% accuracy relative standard deviation less than 3.97%. Furthermore, sensor-based kit easily fabricated and, thus, naked-eye realized onsite. This study can provide universal approach rapid visual complicated matrices.

Language: Английский

Citations

0
A multi-loop aptamer-based fluorescent aptasensor for enhanced detection of 17β-estradiol DOI
Pakawat Kongpreecha,

Arnon Buntha,

Pattama Tongdee

et al.

Analytical Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Newly designed aptamers with 2–5 internal structural loops bind 17β-estradiol more strongly than the 1-loop structure, enhancing aptasensor detection sensitivity.

Language: Английский

Citations

0
Molecularly Imprinted Ratiometric Fluorescent Sensors for Analysis of Pharmaceuticals and Biomarkers DOI Creative Commons

Jing‐Yi Yan,

Shijie Liu,

Dani Sun

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(21), P. 7068 - 7068

Published: Nov. 2, 2024

Currently, analyzing pharmaceuticals and biomarkers is crucial for ensuring medication safety protecting life health, there an urgent need to develop new efficient analytical techniques in view of the limitations traditional methods. Molecularly imprinted ratiometric fluorescent (MI-RFL) sensors have received increasing attention field detection due their high selectivity, sensitivity anti-interference ability, short response time, visualization. This review summarizes recent advances MI-RFL detection. Firstly, fluorescence sources working mechanisms are briefly introduced. On this basis, strategies preparing molecularly polymers, such as dummy template imprinting, nanoimprinting, multi-template stimulus-responsive imprinting strategies, presented. Then, dual- triple-emission types Subsequently, specific applications pharmaceutical analysis highlighted. In addition, innovative point-of-care testing discussed in-depth. Finally, challenges proposed, research outlook development trends prospected.

Language: Английский

Citations

3
Biofuel cell–based self-powered immunosensor for detection of 17β-estradiol by integrating the target-induced biofuel release and biogate immunoassay DOI
Dan Luo,

Jinfei Yi,

Yongju Wu

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(8)

Published: July 22, 2024

Language: Английский

Citations

1
Novel self-powered sandwich type Aptamer sensor for estrogen detection assisted with peroxidase mimic Cu-MOF DOI

Kangping Ning,

Zheng Wang,

Honglan Qin

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140780 - 140780

Published: Aug. 5, 2024

Language: Английский

Citations

0
A rapid microfluidic paper-based chip sensor using ratiometric fluorescence and molecularly imprinted polymers for visual detection of sulfadiazine in actual samples DOI
Yuanzheng Cai, Lingling Cao, Hongmei Cai

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 139, P. 107108 - 107108

Published: Dec. 11, 2024

Language: Английский

Citations

0
Sensitive Detection of 17β-Estradiol in Aqueous Medium via a Ternary Emission Molecularly Imprinted Sensor: A Comprehensive Scientific Research Experiment for Undergraduates DOI
Yu Qiao, Na Wang, Liju Tan

et al.

Journal of Chemical Education, Journal Year: 2024, Volume and Issue: 102(1), P. 27 - 33

Published: Dec. 10, 2024

This study designed a comprehensive short laboratory course for senior undergraduate students to detect type of estradiol (17β-estradiol, E2) in an aqueous medium using ternary emission molecularly imprinted sensor. E2 is the most potent form estrogen, and monitoring its residue levels food environment crucial ensuring safety. Senior majoring chemistry or related areas often need more practical experience deeper understanding scientific research methodologies. To address this issue, employs molecular imprinting technology quantum dot develop polymer (MIP) fluorescence sensors real-time detection trace E2. The includes literature review, sample pretreatment, synthesis, material preparation, characterization, as well data processing analysis. These courses provide with complete facilitate transition postgraduate-level work.

Language: Английский

Citations

0