Microfluidics-Based High-Throughput Single-Cell Analysis of Reactive Oxygen Species and T Cell Exhaustion DOI

Ruotong Rao,

Rui Cao, Wenjun Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 17, 2025

Reactive oxygen species (ROS) play a significant role in cellular signaling and oxidative stress, with elevated levels often linked to T cell exhaustion various pathological conditions, including cancer. However, the relationship between ROS acute myeloid leukemia (AML) remains unexplored. To address this, we developed high-throughput single-cell platform─T reactive analyzer (TEROSA). The system achieved capture efficiency of up 80% throughput 2400 cells enabled dynamic monitoring triple molecules, intracellular mitochondrial superoxide, on-membrane marker PD-1, secreted extracellular H2O2. Our study evaluated device's performance across multiple lines demonstrated its capability assess production at level. In particular, analyzed from AML patients found significantly increased PD-1 expression compared healthy donors, suggesting potential link AML. These findings highlight utility TEROSA advancing our understanding behavior underscore for broader applications analysis.

Language: Английский

Advances in cancer diagnosis and therapy by alginate-based multifunctional hydrogels: A review DOI
Ziwen Wang, Xu Han,

Guowei Sun

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137707 - 137707

Published: Nov. 18, 2024

Language: Английский

Citations

4
Microfluidics-Based High-Throughput Single-Cell Analysis of Reactive Oxygen Species and T Cell Exhaustion DOI

Ruotong Rao,

Rui Cao, Wenjun Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 17, 2025

Reactive oxygen species (ROS) play a significant role in cellular signaling and oxidative stress, with elevated levels often linked to T cell exhaustion various pathological conditions, including cancer. However, the relationship between ROS acute myeloid leukemia (AML) remains unexplored. To address this, we developed high-throughput single-cell platform─T reactive analyzer (TEROSA). The system achieved capture efficiency of up 80% throughput 2400 cells enabled dynamic monitoring triple molecules, intracellular mitochondrial superoxide, on-membrane marker PD-1, secreted extracellular H2O2. Our study evaluated device's performance across multiple lines demonstrated its capability assess production at level. In particular, analyzed from AML patients found significantly increased PD-1 expression compared healthy donors, suggesting potential link AML. These findings highlight utility TEROSA advancing our understanding behavior underscore for broader applications analysis.

Language: Английский

Citations

0