Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance DOI Creative Commons

Yuanyuan Xie,

Yifei Hou,

M. Hu

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 118 - 118

Published: Feb. 17, 2025

Acetylcholinesterase inhibitors (AChEIs), particularly donepezil, are commonly used to treat mild-to-moderate Alzheimer's disease (AD). However, drug accumulation during long-term use could change AChE activity and content, leading peripheral side effects prompting medication discontinuation. there a lack of methods simultaneously determine the content catalytic AChE. By using phosphatidylinositol-specific phospholipase C strip from erythrocyte surfaces, we developed novel method combining surface plasmon resonance fluorescence detection for simultaneous activity, producing stable, reliable, accurate results. The established determination range spans 263.37 ng/mL 3000 (4.05 nM 46.15 nM) concentration, 39.02 mU/mL 1000 activity. Compared traditional methods, this approach simplifies operations, reduces time, expands dynamic range, lowers limits, potentially advancing AChE-related research supporting clinical diagnostics development.

Language: Английский

Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance DOI Creative Commons

Yuanyuan Xie,

Yifei Hou,

M. Hu

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 118 - 118

Published: Feb. 17, 2025

Acetylcholinesterase inhibitors (AChEIs), particularly donepezil, are commonly used to treat mild-to-moderate Alzheimer's disease (AD). However, drug accumulation during long-term use could change AChE activity and content, leading peripheral side effects prompting medication discontinuation. there a lack of methods simultaneously determine the content catalytic AChE. By using phosphatidylinositol-specific phospholipase C strip from erythrocyte surfaces, we developed novel method combining surface plasmon resonance fluorescence detection for simultaneous activity, producing stable, reliable, accurate results. The established determination range spans 263.37 ng/mL 3000 (4.05 nM 46.15 nM) concentration, 39.02 mU/mL 1000 activity. Compared traditional methods, this approach simplifies operations, reduces time, expands dynamic range, lowers limits, potentially advancing AChE-related research supporting clinical diagnostics development.

Language: Английский

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