Effect of repeated HPA axis stimulation on hair cortisol concentration, growth, and behavior in preweaned dairy cattle DOI

J. Kern,

Matthew W. Jorgensen, Jacquelyn P. Boerman

et al.

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Abstract The study objective was to investigate the effect of repeated hypothalamic–pituitary–adrenal (HPA) axis stimulation using synthetic adrenocorticotropic hormone (ACTH) intramuscular injections on hair cortisol concentration, growth, and behavior in preweaned dairy calves. Twenty-seven Holstein calves were assigned nine triads (based sex birth order) randomly 1 3 treatments: 1) control (CON; 2 mL saline weekly); 2) moderate (MOD; alternating Cosyntropin [2 mcg/kg body weight (BW)] or 3) frequent (FREQ; BW] weekly). Calves received their first injection day 0 (7 ± d age). Hair collected from tail switch between days −5 −3 (baseline), 21, 49 analyzed for concentration. To verify endogenous release by during treatment period, saliva 0, 14, 28, 42 before every 15 min h after analysis salivary fitted with accelerometers continuously monitor lying time, number bouts, bout duration throughout study. Growth measures (BW, hip height, width) recorded weekly. Data ANOVA (SAS, Version 9.4), models included fixed effects treatment, time (min day), interaction time. Temperature humidity index as a continuous covariate all models. We observed × (P < 0.0001), whereby concentration lower CON compared MOD FREQ 120 postinjection. While not influenced decreased 21 (1.28 0.03 ng/mL) (0.93 ng/mL). Average BW similar across treatments (CON [59.4 1.09 kg], [58.6 0.98 [57.6 0.96 kg]; P = 0.50). There no evidence suggest difference average daily [18.5 0.23 h/d], [18.6 h/d]; 0.99). These results that HPA through administration increased but did influence

Language: Английский

Running Reverses Chronic Stress‐Induced Changes in Serotonergic Modulation of Hippocampal Granule Cells and Altered Behavioural Responses DOI Creative Commons

Carmen Soto,

Lazaro P. Orihuela,

George Apostol

et al.

European Journal of Neuroscience, Journal Year: 2025, Volume and Issue: 61(7)

Published: March 31, 2025

ABSTRACT Chronic stress increases susceptibility to anxiety and depression disorders, recurrent common psychiatric conditions. Current antidepressant medications have varying degrees of efficacy often multiple side effects limiting treatment adherence. Physical exercise has beneficial on stress‐related mental disorders. However, the underlying mechanisms are unclear. Dentate gyrus granule cells (GCs) excitability may mediate resilience. Here, we expose young adult C57Bl6 mice chronic restraint (CRS) for 14 days followed by 30 running treatment. Behavioural evaluation before after showed that behavioural alterations elicited CRS were mitigated running. Next, evaluated serotonergic modulation GC excitability, as a potential mechanism running‐induced Electrophysiological recordings indicate alters excitability. Utilising (S)‐WAY 100135 Tropisetron, antagonists 5‐HT 1A 3 receptors respectively, show recovers receptor activity lost CRS. Additionally, promotes indirect GCs through activation. Thus, be targets

Language: Английский

Citations

0
Voluntary exercise is a moderately effective mitigator of chronic social isolation stress in two female rodent models DOI

Michael R. Jarcho,

Asavari Gowda,

Annamaria Walden

et al.

Physiology & Behavior, Journal Year: 2025, Volume and Issue: unknown, P. 114902 - 114902

Published: March 1, 2025

Language: Английский

Citations

0
Sex shapes phenotype-linked metabolic signatures of stress exposure in the mouse hypothalamus and pituitary DOI Creative Commons
Lili Wang, Bo Jiang, Xiaopeng Ji

et al.

Neurobiology of Disease, Journal Year: 2025, Volume and Issue: 209, P. 106898 - 106898

Published: April 4, 2025

Language: Английский

Citations

0
Chronic social defeat stress in prairie voles (Microtus ochrogaster): A preclinical model for the study of depression-related phenotypes DOI
Minerva Rodriguez, Anapaula Themann, Israel Garcia-Carachure

et al.

Journal of Affective Disorders, Journal Year: 2024, Volume and Issue: 351, P. 833 - 842

Published: Feb. 8, 2024

Language: Английский

Citations

3
Chronic activation of a negative engram induces behavioral and cellular abnormalities DOI Creative Commons

Alexandra L. Jellinger,

Rebecca L. Suthard, Bingbing Yuan

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: April 17, 2024

Negative memories engage a brain and body-wide stress response in humans that can alter cognition behavior. Prolonged responses induce maladaptive cellular, circuit, systems-level changes lead to pathological states corresponding disorders which mood memory are affected. However, it is unclear if repeated activation of cells processing negative induces similar phenotypes mice. In this study, we used an activity-dependent tagging method access neuronal ensembles assess their molecular characteristics. Sequencing engrams mice revealed positive (male-to-female exposure) (foot shock) upregulated genes linked anti- pro-inflammatory responses, respectively. To investigate the impact persistent engrams, chemogenetically activated them ventral hippocampus over 3 months conducted anxiety memory-related tests. engram increased behaviors both 6- 14-month-old mice, reduced spatial working older impaired fear extinction younger heightened generalization age groups. Immunohistochemistry microglial astrocytic structure number hippocampus. summary, lasting cellular behavioral abnormalities offering insights into effects chronic thinking-like on human health.

Language: Английский

Citations

3
Mouse Bio-behavioral Phenotyping Using a Digital Homecage Framework for Long-timescale, High-resolution, and Multi-factor Data Collection and Analytics DOI Creative Commons
Nicolette Ognjanovski, Anjesh Ghimire, Pho J. Hale

et al.

Published: April 29, 2025

SUMMARY MOTIVATION Long-term monitoring of behavioral and physiological processes is critical for understanding complex brain-based phenomena disorders that develop over extended periods, such as chronic stress, circadian disorders, metabolic conditions. While digital phenotyping available in humans using smart devices, there remains a deficit rodent models. To address this lack long-term phenotyping, we introduce the “Digital Homecage” (DHC) system. Our system uses accessible components designed seamless integration with brain recording technologies. The Digital Homecage allows uninterrupted, long-timescale more than 20 metrics single-housed mice, captured at sub-second resolution via video, operant interactions, wheel-running data. This report demonstrates DHC’s capacity to enable continuous, automated tracking behaviors like actigraphy, sleep, grooming, food choice options weeks, thereby opening up new avenues longitudinal analyses Data collected reveal patterns multiple spontaneous behaviors, aligning known nocturnal tendencies. system’s potential facilitate groundbreaking observations on correlates various neuropsychiatric syndromes aided by open-source software relatively low cost. DHC sets stage community-driven innovation, potentially transforming our approach studying traits function behavior laboratory settings.

Language: Английский

Citations

0
Extremely Low-Frequency Electromagnetic Field (ELF-EMF) Increases Mitochondrial Electron Transport Chain Activities and Ameliorates Depressive Behaviors in Mice DOI Open Access
Masaki Teranishi, Mikako Ito,

Zhizhou Huang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11315 - 11315

Published: Oct. 21, 2024

Compromised mitochondrial electron transport chain (ETC) activities are associated with depression in humans and rodents. However, the effects of enhancement ETC on remain elusive. We recently reported that an extremely low-frequency electromagnetic field (ELF-EMF) as low 10 μT induced hormetic activation complexes human/mouse cultured cells mouse livers. Chronic social defeat stress (CSDS) for consecutive days caused behavioral defects mimicking mice, using ELF-EMF two to six weeks ameliorated them. CSDS variably decreased proteins prefrontal cortex (PFC) days, which were increased by weeks. had no effect oxygen consumption rate PFC but enhanced it. inactivated SOD2 enhancing its acetylation lipid peroxidation PFC. In contrast, activated Sirt3-FoxO3a-SOD2 pathway suppressed peroxidation. Furthermore, markers mitophagy, was The exerted beneficial energy production, antioxidation, dynamics a model depression. envisage is promising therapeutic option

Language: Английский

Citations

3
Association Between the Enriched Environment Level and Serum Brain-Derived Neurotrophic Factor (BDNF) in Patients with Major Depressive Disorder DOI Creative Commons
Andrés Vega-Rosas, Mónica Flores‐Ramos, Gerardo Bernabé Ramírez-Rodríguez

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(11), P. 1137 - 1137

Published: Nov. 13, 2024

Major Depressive Disorder (MDD) is a neuropsychiatric condition whose neurobiological characteristics include alterations in brain plasticity, modulated by Brain-Derived Neurotrophic Factor (BDNF). In animal models, environmental enrichment promotes neuroplasticity and reduces depressive-like behaviors. humans, we proposed to assess the level of Enriched Environment (EE) using questionnaire that includes different domains EE (cognitive, social, physical), which named Indicator (EEI).

Language: Английский

Citations

2
Prevalence of stress, anxiety and depression in the context of climate change among newly recruited contract teachers in Morocco DOI Creative Commons
Nadia Meskini, Mouloud Lamtai, Anis Sfendla

et al.

E3S Web of Conferences, Journal Year: 2023, Volume and Issue: 412, P. 01073 - 01073

Published: Jan. 1, 2023

Teaching has been identified as one of a highly stressful jobs. As known, this job is linked to development neuropsychiatric. However, there little background about the assessment these problems among Moroccan teachers. This study aims measure prevalence stress, anxiety and depression 150 newly recruited contractual primary teachers in KHEMISSET city using three international scales. The perceived stress scale (PSS) for hospital (HADS) Mini International Neuropsychiatric Interview (MINI) depression. results show that 95.9% are stressed. In contrast, 94.9% 72.4% do not suffer from anxiety, respectively. Additionally, was no significant difference scores between male female (p > 0.05). contract Morocco, specifically context climate change, can be influenced by various factors. conclusion, suggest more susceptible suitable steps should taken enhance mental health teachers, thus guaranteeing good quality education.

Language: Английский

Citations

4
Integrative systems neuroimmunology reveals leukocyte-expressing PAX6 as a critical predictor of major depressive disorder DOI

Haroldo Dutra Dias,

Anny Silva Adri,

Adriel Leal Nóbile

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Major depressive disorder (MDD) is a complex psychiatric condition with significant global impact. This study applied genomic-driven integrative systems neuroimmunology approach to analyze transcriptomic data from 3,114 individuals (1,877 MDD patients and 1,237 controls). The analysis revealed neuroimmunological alterations, indicating cross-talk between the immune nervous in peripheral blood mononuclear cells (PBMCs) specific brain regions. Among 31 shared genes, NEGR1, PPP6C, SORCS3, PAX6 emerged as predictors of patients' PBMCs. Notably, was also identified differentially expressed gene (DEG) amygdala, while SORCS3 showed no differential expression other central system (CNS) Validation by immunophenotyping mouse model chronic stress demonstrated increased PBMCs, previously associated GWAS studies. Collectively, our findings suggest existence modules across system, highlighting potential therapeutic target MDD.

Language: Английский

Citations

1