Zebrafish SETD3 mediated ubiquitination of phosphoprotein limits spring viremia of carp virus infection

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 139, P. 108870 - 108870

Published: June 1, 2023

Language: Английский

Identification and analysis of the interaction network of African swine fever virus D1133L with host proteins

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 3, 2022

African swine fever (ASF) is a contagious and lethal hemorrhagic disease in pigs; its spread results huge economic losses to the global pig industry. ASF virus (ASFV) large double-stranded DNA encoding >150 open reading frames. Among them, ASFV-encoded D1133L was predicted be helicase but specific function remains unknown. Since virus-host protein interactions are key understanding viral function, we used co-immunoprecipitation combined with liquid chromatography-mass spectrometry investigate D1133L. This study describes interaction network of ASFV porcine kidney PK-15 cells. Overall, 1,471 host proteins that potentially interact identified. Based on these proteins, protein-protein constructed. Gene ontology Kyoto Encyclopedia Genes Genomes enrichment analyses showed cellular D1133L-interacted involved ribosome, spliceosome, RNA transport, oxidative phosphorylation, proteasome, replication. Vimentin (VIM), tripartite motif-containing 21 (TRIM21), Tu translation elongation factor (TUFM) were confirmed vitro. VIM or TRIM21 overexpression significantly promoted replication, TUFM inhibited These help elucidate functions potential mechanisms underlying

Language: Английский

CRISPR-Mediated Library Screening of Gene-Knockout Cell Lines for Investigating Antiviral Innate Immunity

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 51 - 60

Published: Aug. 27, 2024

Language: Английский