Preparation of a biomimetic bi-layer chitosan wound dressing composed of A-PRF/sponge layer and L-arginine/nanofiber

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 292, P. 119648 - 119648

Published: May 23, 2022

Language: Английский

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The “ideal protein” concept is not ideal in animal nutrition

Experimental Biology and Medicine, Journal Year: 2022, Volume and Issue: 247(13), P. 1191 - 1201

Published: April 11, 2022

Amino acids (AAs) are required for syntheses of proteins and low-molecular-weight substances with enormous physiological importance. Since 1912, AAs have been classified as nutritionally essential amino (EAAs) or nonessential (NEAAs) animals. EAAs those that either not synthesized insufficiently de novo in the organisms. It was assumed all NEAAs (now known synthesizable animal cells [AASAs]) were formed sufficiently animals needed diets. However, studies over past three decades shown sufficient dietary AASAs (e.g. glutamine, glutamate, glycine, proline) necessary maximum growth optimum health pigs, chickens, fish. Thus, concept “ideal protein” (protein an optimal EAA pattern precisely meets needs animals), which originally proposed 1950s but ignored AASAs, is ideal nutrition. Ideal diets must provide physiologically AAs. Improved patterns swine chickens well zoo companion recent years. Animal-sourced feedstuffs supply abundant (including proline, 4-hydroxyproline, taurine) swine, poultry, fish, crustaceans to improve their growth, development, reproduction, health, while sustaining global production. Nutritionists should move beyond consider ratios amounts proteinogenic mammals, birds, aquatic animals, and, case carnivores, also taurine.

Language: Английский

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Dietary supplementation with L-citrulline improves placental angiogenesis and embryonic survival in gilts

Experimental Biology and Medicine, Journal Year: 2023, Volume and Issue: 248(8), P. 702 - 711

Published: April 1, 2023

This study was conducted with gilts as an animal model to test the hypothesis that dietary supplementation L-citrulline (Cit) improves placental angiogenesis and embryonic survival. Between Days 14 25 of gestation, each gilt fed a corn- soybean-meal-based diet (2 kg/day) supplemented 0.4% Cit or isonitrogenous amount L-alanine (Control). On Day were hysterectomized obtain conceptuses. Amniotic allantoic fluids placentae analyzed for NOx [stable oxidation products nitric oxide (NO)], polyamines, amino acids (AAs). Placentae also syntheses NO polyamines; concentrations AAs related metabolites; expression angiogenic factors aquaporins (AQPs). Compared control group, increased ( P < 0.01) number viable fetuses by 2.0 per litter, diameter blood vessels (21% 24%, respectively), weight (15%), total amniotic fluid volumes (20% 47%, respectively). enzymatic activities GTP-cyclohydrolase-1 (32%) ornithine decarboxylase (27%) in placentae; (29%) polyamines (26%); (19%), tetrahydrobiopterin (28%), (22%), cAMP (26%), cGMP (24%) amounts (22–40%), (23–40%), (16–255%), glucose (22–44%), fructose (22–43%) fluids. Furthermore, 0.05) mRNA levels eNOS [84%], GTP-CH1 [55%], PGF [61%], VEGFA120 [26%], VEGFR2 [137%], well AQPs – AQP1 [105%], AQP3 [53%], AQP5 [77%], AQP8 [57%], AQP9 [31%]). Collectively, enhanced polyamine improve conceptus development

Language: Английский

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Perspectives, past, present and future: the proline cycle/proline-collagen regulatory axis

Amino Acids, Journal Year: 2021, Volume and Issue: 53(12), P. 1967 - 1975

Published: Nov. 26, 2021

In the 35 years since introduction of "proline cycle", its relevance to human tumors has been widely established. These connections are based on a variety mechanisms discovered by many laboratories and have stimulated search for small molecule inhibitors treat cancer or metastases. addition, multi-layered proline cycle role hydroxyproline in collagen provide an important regulatory link between extracellular matrix metabolism.

Language: Английский

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Osteopontin (OPN)/Secreted Phosphoprotein 1 (SPP1) Binds Integrins to Activate Transport of Ions Across the Porcine Placenta

Frontiers in Bioscience-Landmark, Journal Year: 2022, Volume and Issue: 27(4)

Published: April 1, 2022

Background: Fetal-placental development depends on a continuous and efficient supply of nutrients from maternal blood that are acquired by exchange through the placenta. However, placenta is low permeability barrier, effective transport substances specific mechanisms. Active requires ions or be moved against an electrical and/or concentration gradient. In pigs, active occurs across chorioallantois to produce electrochemical gradient changes throughout gestation. The aim this study was utilize Ussing chambers detect regulation ion porcine factor(s) within uterine-placental environment pigs. Methods: For measurement transchorioallantoic voltage potential as index placenta, pieces chorioallantoic tissue Day 60 gestation were mounted into cassettes chambers, treatments added mucasal side tissue. Treatments included: (1) media incubated with (placenta-conditioned media); (2) osteopontin/secreted phosphoprotein 1 (OPN/SPP1) purified cow’s milk; (3) placenta-conditioned which OPN/SPP1 removed; (4) recombinant rat OPN intact RGD integrin binding sequence mutated RAD sequence. Ouabain both sides chamber. Immunofluorescence utilized localize beta 3 integrin, aquaporin 8 in placental tissues, lung, kidney small intestine. Results: membranes had greater transepithelial presence media, indicating molecule(s) released increased milk When removed did not increase. Recombinant does bind integrins (RAD) increase Ouabain, inhibiter sodium-potassium pump, ablated Conclusions: present documents novel pericellular matrix role for

Language: Английский

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On-Demand Dissociable Antibacterial Self-Healing Hydrogel with Rapid Arginine-Related Biosynthesis Capacity to Promote Full-Thickness Bacteria-Infected Wound Healing

Industrial & Engineering Chemistry Research, Journal Year: 2023, Volume and Issue: 62(19), P. 7492 - 7503

Published: May 4, 2023

Developing a self-healing hydrogel wound dressing with intrinsic antibacterial and on-demand dissociable properties is highly desired yet challenging in clinician applications to enhance angiogenesis collagen growth for rapid healing. Herein, we present thermoresponsive antimicrobial property based on poly(N-isopropylacrylamide)-derived ABA triblock copolymer (TNOTN) an aldehyde β-cyclodextrin (ACD) healing of full-thickness infected wounds. The prepared TNOTN–ACD exhibits excellent thermal responsiveness well-defined thermal-induced sol–gel transition, allowing situ gelling removal via temperature switch. Meanwhile, the dynamic Schiff base bonds formed between TNOTN ACD endow properties, which can maintain structural integrity constant effective performance. hydrogels have good against both S. aureus E. coli, as well biocompatibility hemocompatibility. More importantly, vivo spatial metabolomics revealed that could accelerate deposition arginine-related biosynthesis pathways, thereby effectively promoting closure bacteria-infected skin defect.

Language: Английский

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Microarray analysis reveals an important role for dietary L-arginine in regulating global gene expression in porcine placentae during early gestation

Frontiers in Bioscience-Landmark, Journal Year: 2022, Volume and Issue: 27(1)

Published: Jan. 18, 2022

Background: Increasing the dietary provision of L-arginine to pregnant swine beginning at Day 14 gestation enhances embryonic survival, but underlying mechanisms are largely unknown. Objective: This study determined effects supplementation with 0.8% gilts between Days and 25 on global expression genes in their placentae. Methods: Between 24 gestation, were fed 2 kg a corn- soybean meal-based diet (containing 12.0% crude protein 0.70% Arg) supplemented or without (0.0%; 1.64% L-alanine as isonitrogenous control). On 30 min after consumption top dressing containing 8 g 16.4 L-alanine, underwent hysterectomy obtain placentae, which snap-frozen liquid nitrogen. Total RNAs extracted from frozen tissues used for microarray analysis based 44-K Agilent porcine gene platform. Results: L-Arginine affected placental 575 genes, 146 being up-regulated 429 down-regulated. These differentially expressed play important roles nutrient metabolism, polyamine production, synthesis, proteolysis, angiogenesis, immune development, anti-oxidative responses, adhesion force chorioallantoic membrane endometrial epithelium, well functions insulin, transforming growth factor beta, Notch signaling pathways. Conclusion: Dietary plays an role regulating gilts. Our findings help elucidate responsible beneficial effect improving embryonic/fetal survival swine.

Language: Английский

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Branched-chain amino acids regulate intracellular protein turnover in porcine mammary epithelial cells

Amino Acids, Journal Year: 2022, Volume and Issue: 54(11), P. 1491 - 1504

Published: Sept. 9, 2022

Language: Английский

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Edible Acid–Base Complexes of Caffeic Acid with Histidine and Arginine Exhibit Enhanced Antimicrobial and Antioxidant Characteristics

ACS Food Science & Technology, Journal Year: 2022, Volume and Issue: 2(2), P. 368 - 377

Published: Feb. 3, 2022

Caffeic acid (CA), an edible plant-derived polyphenol, is known for its various beneficial characteristics such as antimicrobial, antioxidant, and anti-inflammatory. Recently, our group reported a facile acid–base complexation of CA with dopamine (Dopa) to circumvent poor water solubility. Despite the promising biological CA–Dopa complex, Dopa permitted only intravenous but not oral administration. In present study, harness advantages toward nutraceuticals food preservatives, we prepared complexes basic amino acids histidine arginine. The as-prepared CA–amino have been characterized using FT-IR, UV–visible, 1H, 13C, 2D 1H–1H NOESY NMR spectroscopic studies. addition antibacterial activity against Escherichia coli Staphylococcus aureus, also shown high antioxidant ascertained by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay. Further, in vitro cytotoxicity superoxide dismutase studies over mouse fibroblast cells signified biocompatibility radical scavenging performance complexes. Finally, α-amylase assay has non-interference metabolic function enzyme, thereby proving their potential additives pharmaceutic, food, agro sectors.

Language: Английский

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Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 8, 2022

Most embryonic loss in pigs occurs before d 30 of gestation. Dietary supplementation with L-arginine (Arg) during early gestation can enhance the survival and development conceptuses (embryo/fetus its extra-embryonic membranes) gilts. However, underlying mechanisms remain largely unknown. Between 14 gestation, each gilt was fed daily 2 kg a corn- soybean-meal based diet (12% crude protein) supplemented either 0.4% Arg (as Arg-HCl) or an isonitrogenous amount L-alanine (Control). There were 10 gilts per treatment group. On Arg-HCl min they hysterectomized, followed by collection placentae, embryos, fetal membranes, fluids. Amniotic allantoic fluids analyzed for nitrite nitrate [NOx; stable oxidation products nitric oxide (NO)], polyamines, amino acids. Placentae syntheses NO water acid transport, concentrations acid-related metabolites, expression angiogenic factors aquaporins (AQPs). Compared to control group, increased (P < 0.05) number viable fetuses 1.9 litter, diameter placental blood vessels (+ 25.9% + 17.0% respectively), 18.5%), total weight 36.5%), 33.5%), crown-to-rump length 4.7%), amniotic fluid volumes 44.6% 75.5% respectively). gilts, activities GTP cyclohydrolase-1 33.1%) ornithine decarboxylase 29.3%); 26.2%) polyamines 28.9%); NOx 22.5%), tetrahydrobiopterin 21.1%), 20.4%), cAMP 27.7%), cGMP 24.7%); amounts 61.7% 96.8%), 60.7% 88.7%), acids 39% 118%), glucose 60.5% 62.6%), fructose 41.4% 57.0%) fluids; transport 33.9%), 78.4%), glutamine 89.9%), glycine 89.6%). Furthermore, mRNA levels [VEGFA120 117%), VEGFR1 445%), VEGFR2 373%), PGF 197%), GCH1 126%)] AQPs [AQP1 280%), AQP3 137%), AQP5 172%), AQP8 165%), AQP9 127%)]. Supplementing conventional between enhanced polyamine syntheses, angiogenesis, improve conceptus survival.

Language: Английский

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