Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Nov. 3, 2022
Abstract
The
changes
in
the
expression
profile
of
angiogenicgenes
and
serum
biomarkers
were
investigated
cows
with
retained
placenta
after
normal
parturition.
Retained
fetal
membranes
(RFM)
are
considered
one
main
reproductive
disorders
dairy
cattle.
Highlight
effects
Excede®
(Ceftiofur
Crystalline
Free
Acid:
Zoetis,
USA)
combination
Oxytocin
(Oxytocin®,
each
ml
contains
10
IU
oxytocin,
ADWIA,
Egypt),
Estrumate®
(250µg
cloprostenol:
synthetic
analogue
PGF2ά)
Flunixin
Meglumine®
(nonsteroidal
anti-inflammatory
drug)
on
performance
cows.
A
total
16
allocated
into
two
groups:
group
1
(n
=
8)
that
have
a
loosed
placenta,
2
8).
for
more
than
24
h
Blood
EDTA
was
collected
from
tail
vein
studying
angiogenic
proteins
including
vascular
endothelial
growth
factor
(VEGF),
receptor1
(VEGFR1),
RANTES
genes.
Serum
at
one-week
interval
before/after
parturition
study
biochemical
changes.
VEGF
decreased
ten
folds
normally
dropped
placentae
(p
˂
0.0001).
receptor2
(VEGFR2)
showed
threefold
increase
(p˂0.01).
increased
rather
by
about
7
Phosphorus
significantly
lower
(p˂0.05)
RFM
week
albumin,
TG,
HDL,
cholesterol
before,
at,
CPK
higher
(p˂0.05).
Increased
values
MDA
CAT,
SOD,
R-GSH
detected
blood
suffering
Reproductive
parameters
all
studied
days
to
first
estrous,
number
service
per
conception
pregnancy
rate.
All
demonstrated
significant
(P
<
0.05)
between
treated
animals.
translation
its
receptor
RANTESmRNA
time
could
be
proxy
prediction
(RFM).
In
same
line,
activity
proteins,
cholesterol,
would
useful
Altogether,
obtained
results
indicated
successfully
without
any
interference
systemic
injection
cephalosporin
caring
temperature.
criteria
estrus,
services
conception,
rates.
among
also
can
used
treat
placental
retention
animal's
Frontiers in Bioscience-Landmark,
Journal Year:
2022,
Volume and Issue:
27(3)
Published: March 5, 2022
Background:
Dietary
supplementation
with
L-arginine
(Arg)
has
been
shown
to
increase
the
volume
of
fetal
fluids
in
gestating
swine.
Aquaporins
(AQPs),
known
as
water
channel
proteins,
are
essential
for
embryonic
growth
and
development.
It
was
not
if
Arg
mediates
transport
through
AQPs
porcine
conceptus
trophectoderm
(pTr2)
cells.
Methods:
pTr2
cells
derived
from
pregnant
gilts
on
day
12
gestation
were
cultured
customized
Arg-free
Dulbecco’s
modified
Eagle’s
Ham
medium
(DMEM)
supplemented
either
0.00,
0.25,
or
0.50
mM
Arg.
Results:
treatment
increased
expression
AQP3,
which
abundantly
expressed
at
both
mRNA
protein
levels.
also
iNOS
synthesis
nitric
oxide
(NO)
The
presence
Nω-nitro-L-arginine
methyl
ester
hydrochloride
(L-NAME;
an
inhibitor
NO
synthase)
significantly
attenuated
Arg-induced
AQP3.
Furthermore,
concentrations
cAMP
abundances
phosphorylated
cAMP-dependent
kinase
A
(PKA),
PKA
α/β/γ,
CREB.
These
effects
mimicked
by
Forskolin
(a
cell-permeable
activator
adenylyl
cyclase),
but
inhibited
H-89
(an
kinase).
Conclusions:
results
this
study
demonstrate
that
regulates
AQP3
promotes
NO-
signaling
pathways.
Animal Feed Science and Technology,
Journal Year:
2023,
Volume and Issue:
306, P. 115807 - 115807
Published: Nov. 2, 2023
Arginine
(Arg)
supplementation
for
gestating
and
lactating
sows
seems
to
play
a
primary
role
in
litter
development
by
promoting
placental
vascularization
improving
colostrum
milk
quality.
The
aim
of
this
study
was
investigate
the
effects
dietary
standardized
ileal
digestible
Arg
(SID
Arg)
on
productive
performance
using
meta-analytical
approach.
A
total
19
studies
conducted
between
2007-2020
which
tested
effect
additional
SID
diets
were
chosen
systematic
literature
search.
Data
regarding
backfat
thickness,
number
piglets
born
live
born,
birthweight
weaning
weight
extrapolated
from
each
experimental
group
expressed
as
percentage
value
control
within
studies.
treated
compared
(Additional
Arg).
Linear
quadratic
models
built
mixed
procedure
utilizing
Minitab®
software
including
Additional
Arg,
parity
feed
intake
classes
period
fixed
factors,
random
factor.
groups
higher
than
doses
suggested
National
Research
Council
(NRC;
2012)
(4.20-8.90
g/kg
vs
3.2-7.9
g/kg,
respectively).
response
observed
efficiency
(PE;
P=0.003;
a=0.0019),
(P=0.027;
a=-0.0003),
(P=0.005;
a=-0.0006)
thickness
loss
(P=0.057;
a=0.0029).
linear
increase
plasma
concentration
Pro
(P=0.004;
b=0.120),
Orn
(P=0.002;
b=0.284)
(P=0.001;
b=0.425),
decrease
urea
(P=0.037;
b=-0.063)
with
an
increasing
level
Arg.
There
no
placenta
weight,
birthweight,
individual
piglet
at
birth
weaning,
size
average
daily
gain
during
lactation.
estimated
requirements
PE,
reducing
loss,
11.17
13.03
14.83
15.71
feed,
respectively.
This
meta-analysis
highlighted
importance
redefining
dosage
ranging
improve
reproductive
performance.
Animals,
Journal Year:
2024,
Volume and Issue:
14(5), P. 689 - 689
Published: Feb. 22, 2024
The
blood
vessels
of
the
placenta
are
crucial
for
fetal
growth.
Here,
lower
vessel
density
and
ornithine
(Orn)
content
were
observed
in
placentae
low-birth-weight
fetuses
versus
normal-birth-weight
at
day
75
gestation.
Furthermore,
Orn
decreased
from
to
110
To
investigate
role
placental
angiogenesis,
48
gilts
(Bama
pig)
allocated
into
four
groups.
control
group
fed
a
basal
diet
(CON
group),
while
those
experimental
groups
supplemented
with
0.05%
(0.05%
0.10%
(0.10%
0.15%
(0.15%
respectively.
results
showed
that
exhibited
increased
birth
weight
piglets
compared
CON
group.
Moreover,
was
higher
than
densities
placenta.
Mechanistically,
facilitated
angiogenesis
by
regulating
vascular
endothelial
growth
factor-A
(VEGF-A).
maternal
supplementation
during
gestation
jejunal
ileal
villi
height
concentrations
colonic
propionate
butyrate
suckling
piglets.
Collectively,
these
promotes
improves
intestinal
development
Translational Animal Science,
Journal Year:
2023,
Volume and Issue:
7(1)
Published: Jan. 1, 2023
Nine
pregnant
mares
(18.2
±
0.7
yr;
493.82
12.74
kg
body
weight
[BW])
were
used
to
test
the
hypothesis
that
dietary
supplementation
of
l-arginine
would
enhance
placental
vascularity
and
nutrient
transport
throughout
gestation
in
aged
mares.
Mares
balanced
by
age,
BW,
stallion
pairing,
assigned
randomly
treatments
either
supplemental
(50
mg/kg
BW;
n
=
7)
or
l-alanine
(100
6;
isonitrogenous
control).
individually
fed
concentrate
top-dressed
with
respective
amino
acid
treatment
plus
ad
libitum
access
Coastal
Bermudagrass
hay.
Treatments
began
on
day
14
terminated
at
parturition.
Mare
condition
score
(BCS),
rump
fat
determined,
percentage
was
calculated
every
28
d
adjusted
accordingly.
Doppler
blood
flow
measurements
including
resistance
index
(RI)
pulsatility
for
uterine
artery
ipsilateral
horn
obtained
beginning
21
continued
7
until
154
gestation,
prior
Parturition
attended
foaling
variables
measures
recorded.
Placental
tissue
from
analyzed
histologically
assess
cell-specific
localization
vascular
endothelial
growth
factor
(VEGF)
cationic
transporter
1
(SLC7A1)
proteins.
Semiquantitative
analyses
performed
using
10
nonoverlapping
images
per
sample
fixed
a
10×
field
(Fiji
ImageJ
v1.2).
performance
data
PROC
MIXED
SAS
GLM.
Gestation
length
parturition
not
influenced
(P
>
0.05)
arginine.
Compared
arginine-supplemented
mares,
control
had
thicker
layer
<
0.01)
greater
percent
0.03),
BCS
Arginine-supplemented
lower
RI
than
0.01).
Body
length,
height,
BW
foals
birth,
as
well
volume,
immunohistochemical
staining
VEGF
SLC7A1
parturition,
affected
maternal
arginine
supplementation.
These
results
indicate
BW)
is
safe
gestating
A
larger
number
required
extend
knowledge
effects
embryonic/fetal
survival
Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Nov. 3, 2022
Abstract
The
changes
in
the
expression
profile
of
angiogenicgenes
and
serum
biomarkers
were
investigated
cows
with
retained
placenta
after
normal
parturition.
Retained
fetal
membranes
(RFM)
are
considered
one
main
reproductive
disorders
dairy
cattle.
Highlight
effects
Excede®
(Ceftiofur
Crystalline
Free
Acid:
Zoetis,
USA)
combination
Oxytocin
(Oxytocin®,
each
ml
contains
10
IU
oxytocin,
ADWIA,
Egypt),
Estrumate®
(250µg
cloprostenol:
synthetic
analogue
PGF2ά)
Flunixin
Meglumine®
(nonsteroidal
anti-inflammatory
drug)
on
performance
cows.
A
total
16
allocated
into
two
groups:
group
1
(n
=
8)
that
have
a
loosed
placenta,
2
8).
for
more
than
24
h
Blood
EDTA
was
collected
from
tail
vein
studying
angiogenic
proteins
including
vascular
endothelial
growth
factor
(VEGF),
receptor1
(VEGFR1),
RANTES
genes.
Serum
at
one-week
interval
before/after
parturition
study
biochemical
changes.
VEGF
decreased
ten
folds
normally
dropped
placentae
(p
˂
0.0001).
receptor2
(VEGFR2)
showed
threefold
increase
(p˂0.01).
increased
rather
by
about
7
Phosphorus
significantly
lower
(p˂0.05)
RFM
week
albumin,
TG,
HDL,
cholesterol
before,
at,
CPK
higher
(p˂0.05).
Increased
values
MDA
CAT,
SOD,
R-GSH
detected
blood
suffering
Reproductive
parameters
all
studied
days
to
first
estrous,
number
service
per
conception
pregnancy
rate.
All
demonstrated
significant
(P
<
0.05)
between
treated
animals.
translation
its
receptor
RANTESmRNA
time
could
be
proxy
prediction
(RFM).
In
same
line,
activity
proteins,
cholesterol,
would
useful
Altogether,
obtained
results
indicated
successfully
without
any
interference
systemic
injection
cephalosporin
caring
temperature.
criteria
estrus,
services
conception,
rates.
among
also
can
used
treat
placental
retention
animal's
