Purification and characterization of a thermophilic NAD⁺‐dependent lactate dehydrogenase from Moorella thermoacetica

FEBS Open Bio, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Oxidation of lactate under anaerobic dark fermentative conditions poses an energetic problem. The redox potential the lactate/pyruvate couple is too electropositive to reduce physiological electron carriers NAD(P) + or ferredoxin. However, thermophilic, anaerobic, and acetogenic model organism Moorella thermoacetica can grow on but was suggested have a NAD ‐dependent dehydrogenase (LDH), based enzyme assays in cell‐free extract. LDHs thermophilic bacteria are barely characterized huge biotechnological potential. Here, we purified LDH from M. by classical chromatography. Lactate‐dependent reduction observed with high rates. Electron bifurcation not observed. At pH 8 65 °C, had specific activity 60 U·mg −1 for oxidation, NADH‐driven pyruvate around four times faster 237 . Since formation preferred enzyme, further modifications be improve kinetics this making it promising candidate applications.

Language: Английский

---

Bio-Economy and Development of Human Capital: Provision of Population Health in Regions of Russia

Vestnik of the Plekhanov Russian University of Economics, Journal Year: 2025, Volume and Issue: 2, P. 101 - 117

Published: March 18, 2025

The goal of the article is to study interconnection between bio-economy shaping, development human capital and population health in regions Russia. Certain indicators were analyzed federal areas Russian Federation 21st century. research showed a trend reduction death-rate employable people (death number per 100.000 people). At same time it was found that NorthCaucasian area considerably lower comparison with other Russia general (by Mann – Whitney non-parametric criterion). Dynamics sick-rate illustrated by key classes illnesses 2000–2022. Findings underline socially important TB among them are for health, which considered as one most significant components plays principle role regional economy. Analysis statistic data shows reliable cut numbers from 2010 2022 all areas. authors show cyclic interdependence bioeconomy functioning provision possible ways. put forward model condition capital, characteristics public system operation prospects development, gives an opportunity assess potential bio-economy.

Language: Английский

---

Extremophiles in Climate Change Mitigation: Harnessing Resilient Microbes for a Sustainable Future

Microorganisms for sustainability, Journal Year: 2025, Volume and Issue: unknown, P. 247 - 271

Published: Jan. 1, 2025

Language: Английский

---

Transcriptomic and proteomic insights into feather keratin degradation by Fervidobacterium

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Keratin, one of the most recalcitrant and abundant proteins on Earth, constitutes a challenging underutilized material for poultry industry. Although it resists degradation by commonly available enzymes, natural breakdown occurs through action certain fungi bacteria. This process remains poorly understood, only few thermophilic anaerobic bacteria are known to effectively degrade keratin. Some members genus Fervidobacterium have been demonstrated be effective at degrading feather keratin under high temperatures anoxic conditions. However, comprehensive evaluation their keratinolytic capabilities lacking, leaving potential largely underexplored. In this study, we assessed activity all strains. Six strains were active against substrate, namely changbaicum CBS-1T, islandicum H-21T, pennivorans T, DSM9078T, sp. GSH, 21710. These used in comparative proteomics analysis, grown with either glucose or chicken feathers as sole carbon source. Similarly, three efficient strains, H-21T underwent an in-depth transcriptomics analysis. Among numerous upregulated overexpressed genes identified when comparing feather-grown glucose-grown cells, oxidoreductases peptidases key enzymes process, suggesting application enzymatic cocktails

Language: Английский

---

A new age in structural S-layer biology - Experimental and in silico milestones

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110205 - 110205

Published: May 1, 2025

Surface (S-) layer proteins, considered as the most abundant proteins in nature, perform diverse and essential biological roles many bacteria archaea. Their functions range from providing structural support, maintaining cell shape, protecting against extreme environments to acting a surface display matrix for biologically active molecules, such S-layer protein-bound glycans, which facilitate interspecies interactions cellular communication both health disease. The intricate, symmetric, nanometer-scale patterns of lattices have long fascinated biologists, yet only recent methodological advances revealed detailed molecular insights. These include deeper understanding domain organization, wall anchoring mechanisms, how nascent are incorporated into existing lattices. Significant progress sample preparation high-resolution imaging has led precise characterization S-layers across various bacterial archaeal species. Furthermore, advent deep learning-based structure prediction enabled modeling several largely uncultured microbial lineages. This review summarizes major achievements protein research over past five years, presenting them with typical workflow experimental determination. For first time, it also explores breakthroughs computational modelling offers an outlook on silico methods may further advance our architecture.

Language: Английский

---

Construction of Promoter-Ribosome Binding Site Library for Manipulating Gene Expression in the Hyperthermophilic Archaeon Thermococcus kodakarensis

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 27, 2025

Extremely thermophilic archaea, such as Thermococcus kodakarensis, possess biotechnological potential sources of either thermostable enzymes or biotransformation processes under high temperature. However, the absence a tool for fine-tuning gene expression impedes its advancement platform organism. Here, we constructed genetic element library in T. which includes constitutive promoters, inducible and ribosome binding site (RBS). The promoter consisted 76 promoters with strengths spanning ∼8 × 103-fold dynamic range 22 consisting 15 maltodextrin-inducible 7 pressure-inducible maximum induction strength achieving ∼8-fold increase. We also generated an RBS containing 31 different sequences, translation covering ∼5-fold range. Utilizing characterized identified library, hydrostatic toxin-antitoxin (TA) system toxin counterselectable cassette regulated by antitoxin switch modifications kodakarensis to realize markerless disruption directly rich medium. Moreover, rational control relative levels TA enhanced knockout efficiency. then replaced native genes associated hydrogen production pathways various types resulting 2.68-fold increase yield (59.4 mmol liter-1 vs 22.2 liter-1). Therefore, toolbox developed this work is highly significant advancing fundamental biological research engineering hyperthermophilic Thermococcales.

Language: Английский

---

Deciphering the enigmatic PilY1 of Acidithiobacillus thiooxidans: An in silico analysis

Biochemistry and Biophysics Reports, Journal Year: 2024, Volume and Issue: 39, P. 101797 - 101797

Published: July 26, 2024

Thirty years since the first report on PilY1 protein in bacteria, only C-terminal domain has been crystallized; there is no study which N-terminal domain, let alone complete protein, crystallized. In our laboratory, we are interested characterizing Type IV Pili (T4P) of

Language: Английский

---

Methanothermobacter thermautotrophicus and Alternative Methanogens: Archaea-Based Production

Advances in biochemical engineering, biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

---

Extremophiles and Related Extremozymes: Their Structure-Function Relationship in Industrial Applications

Industrial Biotechnology, Journal Year: 2024, Volume and Issue: 20(6), P. 279 - 295

Published: Dec. 1, 2024

Language: Английский

---