The inferred modulation of correlated vaginal microbiota and metabolome by cervical differentially expressed genes across distinct CIN grades DOI Creative Commons
Wenkui Dai, Yu Liu, Xin Jiang

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 2, 2025

In vitro studies have demonstrated the modulation of vaginal microbiota (VM) by cervical peptides which levels varied with status HPV infection and intraepithelial neoplasia (CIN) grades. However, there is a deficiency in population-based investigating VM compositions metabolome differentially expressed genes (DEGs) across different grades CIN. This study included 43 HPV-positive women, classified into low-grade (CIN1, n = 23) high-grade (CIN2 + , 20) groups. Vaginal swabs were collected for both analysis. Cervical exfoliated cells RNA-Seq We identified 258 (DEGs), among 176 CIN1-enriched linked to immune responses, cell chemotaxis, negative regulation migration, B differentiation, activation, proliferation. Eighty-two upregulated CIN2 cohorts associated epidermis development keratinization. Then, we 5,686 paired correlations between DEGs, VM, metabolome, 2,320 involving Lactobacillus. Further analysis revealed Lactobacillus as primary determinant metabolic profiles, followed Gardnerella, Faecalibacterium, Aerococcus Streptococcus, such notable positive correlation D-lactic acid DL-indole-3-lactic acid. Applying mediation analysis, found that mediated association 14 COL4A2, CCBE1 SPON1, production 57 metabolites, including acid, oleic various amino acids. Additional indicated significant effects 79 metabolites on DEGs growth Lactobacillus, Fannyhessea Aerococcus. Our findings provide valuable evidence inferred correlated CIN stages.

Language: Английский

The Promising Biological Role of Postbiotics in Treating Human Infertility DOI
Nader Khani, Amir Hesam Shakeri, Sousan Houshmandi

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Language: Английский

Citations

1
The inferred modulation of correlated vaginal microbiota and metabolome by cervical differentially expressed genes across distinct CIN grades DOI Creative Commons
Wenkui Dai, Yu Liu, Xin Jiang

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 2, 2025

In vitro studies have demonstrated the modulation of vaginal microbiota (VM) by cervical peptides which levels varied with status HPV infection and intraepithelial neoplasia (CIN) grades. However, there is a deficiency in population-based investigating VM compositions metabolome differentially expressed genes (DEGs) across different grades CIN. This study included 43 HPV-positive women, classified into low-grade (CIN1, n = 23) high-grade (CIN2 + , 20) groups. Vaginal swabs were collected for both analysis. Cervical exfoliated cells RNA-Seq We identified 258 (DEGs), among 176 CIN1-enriched linked to immune responses, cell chemotaxis, negative regulation migration, B differentiation, activation, proliferation. Eighty-two upregulated CIN2 cohorts associated epidermis development keratinization. Then, we 5,686 paired correlations between DEGs, VM, metabolome, 2,320 involving Lactobacillus. Further analysis revealed Lactobacillus as primary determinant metabolic profiles, followed Gardnerella, Faecalibacterium, Aerococcus Streptococcus, such notable positive correlation D-lactic acid DL-indole-3-lactic acid. Applying mediation analysis, found that mediated association 14 COL4A2, CCBE1 SPON1, production 57 metabolites, including acid, oleic various amino acids. Additional indicated significant effects 79 metabolites on DEGs growth Lactobacillus, Fannyhessea Aerococcus. Our findings provide valuable evidence inferred correlated CIN stages.

Language: Английский

Citations

0