Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: March 31, 2022

The Pacific oyster, Crassostrea gigas , is widely distributed and substantially important to shellfish cultivation. Oysters with melanin-colored shells soft parts are increasingly popular of high economic value. However, the distribution synthesis melanin in C. remains unclear there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used observe epidermis dark mantle tissue. Melanocytes containing melanosomes were observed below epidermis, suggesting possibility a gradual transfer from tissue epithelium. frozen section technique three staining methods confirm presence melanin. results suggest that enzyme identification (DOPA) suitable method tissues . This study preliminarily identified existence foundation for synthesis. Furthermore, it provides new insights into mechanism shell color formation.

Language: Английский

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Functional Characterization of Cfap206 for Bivalve Ciliogenesis by RNAi and CRISPR/Cas9 Technologies

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: March 24, 2022

Cilia are organelles located on the surface of eukaryotic cells and play important roles in numerous physiological developmental processes. Cilia- flagella-associated proteins (CFAP family) well known due to their close relation assembly functionalization both cilia flagella. In vertebrates, Cfap206 is reported function during motile assembly, its abolished expression could lead abnormal ciliary beating decreased sperm motility. However, there scarcely any information marine invertebrates. Here, through using an ideal shellfish research model, dwarf surf calm Mulinia lateralis , we explored role embryonic ciliogenesis as male gametogenesis. trochophore larvae, effective knocking down or out was detected respectively RNAi CRISPR/Cas9 technology, showing obviously number cilia. During period gonadal maturation, managed deliver dsRNA individuals for 14 days carrier vector feeding. Significant suppression observed, together with impaired motility aberrant tail assembly. The present study provided valuable gene exploration methods M. further enriched understanding Furthermore, by revealing relationship between spermatozoa flagellum, it possible generate sterile populations hermaphroditic organisms improve crossbreeding efficiency.

Language: Английский

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Somatostatin Receptor Gene Functions in Growth Regulation in Bivalve Scallop and Clam

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4813 - 4813

Published: April 28, 2024

Bivalves hold an important role in marine aquaculture and the identification of growth-related genes bivalves could contribute to a better understanding mechanism governing their growth, which may benefit high-yielding bivalve breeding. Somatostatin receptor (SSTR) is conserved negative regulator growth vertebrates. Although SSTR have been identified invertebrates, involvement regulation remains unclear. Here, we seven SSTRs (PySSTRs) Yesso scallop, Patinopecten yessoensis, economically cultured East Asia. Among three PySSTRs (PySSTR-1, -2, -3) expressed adult tissues, PySSTR-1 showed significantly lower expression fast-growing scallops than slow-growing scallops. Then, function this gene was evaluated dwarf surf clams (Mulinia lateralis), potential model lab, via RNA interference (RNAi) through feeding Escherichia coli containing plasmids expressing double-stranded RNAs (dsRNAs) targeting MlSSTR-1. Suppressing MlSSTR-1, homolog M. lateralis, resulted significant increase shell length, width, height, soft tissue weight, muscle weight by 20%, 22%, 79%, 92%, respectively. A transcriptome analysis indicated that up-regulated after MlSSTR-1 inhibition were enriched fat digestion absorption pathway insulin pathway. In summary, systemically P. yessoensis revealed growth-inhibitory SSTR-1 bivalves. This study indicates somatostatin signaling regulation, ingesting dsRNA-expressing bacteria useful way verify candidate target for editing promote be used breeding

Language: Английский

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Abalone Viral Ganglioneuritis

Pathogens, Journal Year: 2020, Volume and Issue: 9(9), P. 720 - 720

Published: Sept. 1, 2020

Abalone viral ganglioneuritis (AVG), caused by Haliotid herpesvirus-1 (HaHV-1; previously called abalone herpesvirus), is a disease that has been responsible for extensive mortalities in wild and farmed significant economic losses Asia Australia since outbreaks occurred the early 2000s. Researchers from Taiwan, China, have conducted numerous studies encompassing HaHV-1 genome sequencing, development of molecular diagnostic tests, evaluation susceptibility various species to AVG as well gene expression upon virus infection. This review presents timeline most research findings on potential future avenues further understand this order develop better management strategies.

Language: Английский

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RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(11), P. 6120 - 6120

Published: June 6, 2021

Porphyrins are a widespread group of pigments in nature which believed to contribute shell colors mollusks. Previous studies have provided candidate genes for porphyrin coloration, however, the linkage analysis between functional and pigmentation remains unclear RNA interference is powerful molecular tool analyzing loss functions vivo alter gene expression. In this study, we used unicellular alga Platymonas subcordiformis Nitzschia closterium f. minutissima as vectors feed oysters with Escherichia coli strain HT115 engineered express double-stranded RNAs targeting specific involved synthesis. A Crassostrea gigas orange was target key haem pathway expression using aforementioned approach. We show here that feeding E. coli, containing dsRNA genes, can cause changes color newly deposited shell. For example, RNAi knockdown CgALAS CgPBGD resulted uroporphyrin from due accumulation pigment oyster’s mantle. The study probed crucial role ALAS PBGD potential production C. gigas.

Language: Английский

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