Spatial-temporal expression analysis of lineage-restricted shell matrix proteins reveals shell field regionalization and distinct cell populations in the slipper snailCrepidula atrasolea DOI Open Access

Rebecca N. Lopez‐Anido,

Grant Batzel, Gabriela Ramírez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 21, 2023

Molluscs are one of the most morphologically diverse clades metazoans, exhibiting an immense diversification calcium carbonate structures, such as shell. Biomineralization calcified shell is dependent on matrix proteins (SMPs). While SMP diversity hypothesized to drive molluscan diversity, we just starting unravel evolutionary history and biology. Here leveraged two complementary model mollusc systems, Crepidula fornicata atrasolea , determine lineage-specificity 185 SMPs. We found that 95% adult C. proteome belongs conserved metazoan orthogroups, with molluscan-restricted orthogroups containing half all SMPs in proteome. The low number -restricted contradicts generally-held notion animal’s biomineralization toolkit dominated by mostly novel genes. Next, selected a subset lineage-restricted for spatial-temporal analysis using situ hybridization chain reaction (HCR) during larval stages . 12 out 18 analyzed expressed field. Notably, these genes present 5 expression patterns, which define at least three distinct cell populations within These results represent comprehensive gastropod age field patterns date. Collectively, data lay foundation future work interrogate molecular mechanisms fate decisions underlying mantle specification diversification.

Language: Английский

Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture DOI Creative Commons
Zhanjiang Liu, Tao Zhou,

Dongya Gao

et al.

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 2, 2022

Major progress has been made with genomic and genetic studies in aquaculture the last decade. However, research on epigenetic regulation of traits is still at an early stage. It apparent that most, if not all, are regulated both levels. This paper reviews recent understanding important such as growth, reproduction, disease resistance, stress responses. Although it challenging to make generalized statements, DNA methylation mostly correlated down-regulation gene expression, especially when promoters enhancers. As such, growth factors their receptors negatively growth; hypomethylation genes for tolerance increased tolerance; male or female sex differentiation leads into males females, respectively. environmental mediated level regulation, environment-induced changes appeared be intergenerationally inherited, but evidences transgenerational inheritance limited.

Language: Английский

Citations

48
Transcriptomic responses reveal impaired physiological performance of the pearl oyster following repeated exposure to marine heatwaves DOI

Guixiang He,

Xinwei Xiong,

Yalan Peng

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 854, P. 158726 - 158726

Published: Sept. 13, 2022

Language: Английский

Citations

39
Development and Evaluation of High-Density SNP Arrays for the Eastern Oyster Crassostrea virginica DOI
Ximing Guo, Jonathan B. Puritz, Zhenwei Wang

et al.

Marine Biotechnology, Journal Year: 2023, Volume and Issue: 25(1), P. 174 - 191

Published: Jan. 9, 2023

Language: Английский

Citations

23
Genome of Kumamoto Oyster Crassostrea sikamea Provides Insights Into Bivalve Evolution and Environmental Adaptation DOI Creative Commons
Sheng Liu, Youli Liu, Ximing Guo

et al.

Evolutionary Applications, Journal Year: 2025, Volume and Issue: 18(4)

Published: April 1, 2025

ABSTRACT The Kumamoto oyster, Crassostrea sikamea , is a marine bivalve naturally distributed along the coasts of southern China and Japan, with hatchery population that has been under domestication in United States since its introduction from Japan 1940s. To understand evolutionary history environmental adaptation, we produced chromosome‐level genome assembly C. conducted whole‐genome resequencing 141 individuals US six wild populations Japan. assembled size 616 Mb covering all 10 chromosomes contig N50 4.21 scaffold 62.25 Mb. Phylogenetic analysis indicated diverged angulata gigas clade about 9.9 million years ago. Synteny revealed significant chromosomal rearrangements during evolution leading to oysters, but remarkable conservation oyster over ~180 years, surprising disparity evolution. three distinct clusters for US, Japanese, Chinese populations, closer Japanese population, confirming origin. No differentiation was detected among five indicating strong gene flow. Between 402 genes exhibited selection signals, including myosin heavy chain were also differentiated domesticated lines eastern suggesting changes these may be important domestic production. Among 768 showing signals between natural China, related stress response are most enriched, responding critical local adaptation. These findings provide insights into as well useful resources comparative genomics genetic improvement cultured stocks.

Language: Английский

Citations

1
Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories DOI Creative Commons
Laura Piovani, Daniel J. Leite, Luis Alfonso Yañez Guerra

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(31)

Published: Aug. 2, 2023

Pelagic larval stages are widespread across animals, yet it is unclear whether larvae were present in the last common ancestor of animals or they evolved multiple times due to selective pressures. Many marine at least superficially similar; small, swim through beating bands cilia, and sense environment with an apical organ. To understand these similarities, we have generated single-cell atlases for from two animal phyla compared their cell types. We found clear similarities among ciliary band cells between neurons organ pointing possible homology structures, suggesting a single origin within Spiralia. also find several clade-specific innovations each larva, including distinct myocytes shell gland oyster larva. Oyster express many recently genes that made previous gene age estimates trochophore too young.

Language: Английский

Citations

14
Exposure to plastic debris alters expression of biomineralization, immune, and stress-related genes in the eastern oyster (Crassostrea virginica) DOI Creative Commons
Laura E. Eierman, Jacob B. Landis

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319165 - e0319165

Published: April 29, 2025

The degradation of marine plastic debris poses a threat to organisms by fragmenting into micro- and nano-scale pieces releasing complex chemical leachate the water. Numerous studies have investigated harms from pollution such as microplastic ingestion exposure single chemicals. However, few examined holistic synergistic impacts mixtures. objective this study was measure changes in gene expression gill gonadal tissue eastern oyster ( Crassostrea virginica ) response during their first year, using RNA-seq explore multiple types physiological responses. Shell polyethylene terephthalate were used substrate for metamorphosis larval oysters settlement tank. Substrate then transferred metal cages outplanted pairs – shell cage onto restoration reefs St. Mary’s River, Maryland, USA. After 10 months growth, collected, removed, sex identified. tissues six each type analyzed RNA-seq. Both samples had altered immune stress-response genes exposure. Genes upregulated enriched ontology functions proteolysis fibrinolysis. Downregulated involved biomineralization growth. One male exposed “feminized” patterns despite developing mature sperm, suggesting can alter shift protandric individuals develop females. Plastic may therefore reduce initiate stress responses, differentiation, impact reproductive output through transcription.

Language: Английский

Citations

0
Toward Understanding Mechanistic Regulation of Body Size and Growth Control in Bivalve Mollusks DOI
A. Mokrani,

J B Li,

Qi Li

et al.

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 28, 2024

ABSTRACT Bivalves possess a pair of valves connected to stretchable ligament that facilitates the opening and closing shell. The growth bioprocess commences when supplemental materials secreted from edge are added early‐constructed Here, we endeavor provide glimpse into physiological responses, mechanistic control, omics applications toward understanding this complex trait. In first section, review studies have been performed investigate effects food availability, temperature, salinity, contaminants, climate change in natural ecosystems under experimental conditions. These conditions affect some internal promotors alter concentration particular neuropeptides neurotransmitters induce neuroendocrinal signals crucial for regulating peculiar process. Besides, predicted concept organs' size control maintaining body homeostasis via intertwining networks, including Hippo pathway. On other hand, discuss findings employing genomics, transcriptomics, proteomics, metabolomics approaches uncover modulation growth‐related traits different bivalve species. We recommend further research decipher organ its intricate relationship with entire homeostasis. Future genetic dissection also recommended identify new key genes major effect profoundly influences trait, facilitating their potential editing develop strains enhanced rates.

Language: Английский

Citations

2
Marine thermal fluctuation induced gluconeogenesis by the transcriptional regulation of CgCREBL2 in Pacific oysters DOI
Qingsong Li, Lei Gao, Lu Liu

et al.

Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 207, P. 116906 - 116906

Published: Sept. 1, 2024

Language: Английский

Citations

2
The adult shell matrix protein repertoire of the marine snail Crepidula is dominated by conserved genes that are also expressed in larvae DOI Creative Commons

Rebecca N. Lopez‐Anido,

Grant Batzel, Gabriela Ramírez

et al.

BMC Ecology and Evolution, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 14, 2024

Language: Английский

Citations

2
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas DOI Creative Commons
Shanshan Yao, Li Li, Xudong Guan

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 19, 2022

The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand genetic architecture defense against pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing before and after-mortality larval samples as well dead surviving adults from viral challenge. Analysis data identified 5,271 SNPs 1,883 regions covering 3,111 genes in larvae, 18,692 28,314 4,863 that were significantly mortalities. Only 1,653 implicated shared larvae adults, suggesting antiviral response or resistance involves different sets differentiated members expanded gene families. Combined analyses previous transcriptomic challenge experiments revealed transcription many mortality-associated was also upregulated infection confirming their importance response. Key immune especially those encoding receptors such TLRs RLRs displayed strong association between variation regulatory region mortality, they may confer through transcriptional modulation. These results point to previously undescribed mechanisms for disease at developmental stages provide candidate polymorphisms are valuable understanding responses breeding resistance.

Language: Английский

Citations

8