Epigenetic regulations under plant stress: A cereals perspective

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 220, P. 105688 - 105688

Published: Feb. 10, 2024

Language: Английский

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Harnessing the potential of non-coding RNA: An insight into its mechanism and interaction in plant biotic stress

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 207, P. 108387 - 108387

Published: Jan. 18, 2024

Language: Английский

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Transcriptome, miRNA, and degradome sequencing reveal the leaf stripe (Pyrenophora graminea) resistance genes in Tibetan hulless barley

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 17, 2025

Barley leaf stripe, a disease mainly caused by Pyrenophora graminea (P. graminea) infection, severely affects barley yield and quality is one of the most widespread diseases in production. However, little known about underlying molecular mechanisms stripe resistance. In this study, transcript expression profiles normal infected leaves resistant Tibetan hulless (Hordeum vulgare L. var. nudum Hook. f.) variety Kunlun 14 susceptible Z1141 were analyzed RNA sequencing (RNA-seq). The results showed total 7,669 5,943 differentially expressed genes (DEGs) found Z1141, respectively, with 8,916 DEGs between Z1141. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analysis identified many significantly enriched categories pathways, which plant-pathogen interaction pathway, containing 102 (100 two novel genes), was found, that very important for study resistance mechanism. Using RNA-seq, small (miRNA-seq) combined degradome (degradome-seq), four pairs associated leaf-stripe miRNAs target obtained, namely Hvu-miR168-5p Argonaute1 (HvAGO1), Hvu-novel-52 growth-regulating factor 6 (HvGRF6), Hvu-miR6195 chemocyanin-like protein (CLP), Hvu-miR159b gibberellin-dependent MYB (GAMYB). Transformation gene HvAGO1 into Arabidopsis verified could against Botrytis cinerea. Then RNA-seq miRNA-seq transformed overexpressed performed. Based on above research results, we constructed Protein-Protein Interaction (PPI) network This lays foundation mechanism provides new targets genetic improvement disease-resistant varieties.

Language: Английский

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Comprehensive in silico analysis to decipher the role of long non-coding RNAs in bread wheat during fusarium head blight and leaf rust interactions

Academia molecular biology and genomics., Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 11, 2025

The advancements in high-throughput sequencing technology have disclosed the substantial role of plant non-coding RNAs (ncRNAs), a relatively lessexplored but potent regulatory component, fine-tuning responsestoabiotic and biotic stresses. Among these ncRNAs, long (lncRNAs) microRNAs (miRNAs) are well-recognized robust elements that can modulate gene regulation at transcriptional, post-transcriptional, epigenetic levels. Emerging empirical evidence supports intricate orchestration miRNA-lncRNA networks regulating distinct aspects plant–pathogen interactions. Leaf rust (LR) fusarium head blight (FHB) two seriousfungal diseases caused by Puccinia triticinaand Fusarium graminearum, which persistently affect wheat growth worldwide. current study employs an silico approach to predict differentially expressed lncRNAs (DElncRNAs) from transcriptome studies under P. F. graminearum infection. A total 595 DElncRNAs were predicted implementing stringent lncRNA selection criterion. disclosure acting as putative miRNA precursors, targets, target mimics highlightsthe interdependence between miRNAs during fungal invasions wheat. In alignment with prior research, sequence homology search exhibited only marginal similarities other related crops. functional annotation cis trans targets unveiled prevalence defence mycotoxin-related processes FHB-infected samples, whereas LR infection primarily elicited oxidative stress polyamine synthesis activities. Thisstudy unveils potential divergence within serves fine resource be harnessed elucidate interplay miRNA-lncRNA-mRNA governing wheat–fungal

Language: Английский

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MicroRNAs in Plants Development and Stress Resistance

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

ABSTRACT Plant growth and development are governed by a rigorously timed sequence of ontogenetic programmes. MicroRNAs (miRNAs), class short noncoding RNAs, function as master regulators gene expression targeting mRNAs for cleavage or direct translational inhibition at the posttranscriptional level in eukaryotes. Numerous miRNA molecules that control significant agronomic properties plants have been found. On one hand, miRNAs target transcription factors (TFs) to determine plant structure, such root development, internode elongation, leaf morphogenesis, sex determination nutrient transition. other alter levels adapt biological abiotic stresses, including fungi, bacteria, viruses, drought, waterlogging, high temperature, low salinity, deficiencies, heavy metals stresses. To fully understand role plants, we review regulatory stress resistance. Beyond that, propose novel can be effectively further studied with artificial (amiRNA) tandem mimics (STTM) delivery vitro used improve crop yield agricultural sustainability.

Language: Английский

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Candidate effectors for leaf rust resistance gene Lr28 identified through transcriptome and in-silico analysis

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 17, 2023

Puccinia spp. causing rust diseases in wheat and other cereals secrete several specialized effector proteins into host cells. Characterization of these their interaction with host's R could greatly help to limit crop losses due diseases. Prediction by combining the transcriptome analysis multiple in-silico approaches is gaining importance revealing pathogenic mechanism. The present study involved identification 13 triticina (Pt) coding sequences (CDSs), through analysis, that were differentially expressed during wheat-leaf interaction; prediction like features using different tools. NCBI-BLAST pathogen-host BLAST (PHI-BLAST) tools used annotate classify based on most closely matched counterpart both databases. Homology between CDSs annotated NCBI database ranged from 79 94% putative effectors plant pathogens PHI-BLAST 24.46 54.35%. Nine had effector-like according EffectorP 3.0 (≥0.546 probability be effector). qRT-PCR expression revealed relative all compatible (HD2329) was maximum at 11 days post inoculation (dpi) incompatible interactions (HD2329 + Lr28) 3 dpi seven 9 five CDSs. These results suggest six (>0.8 as per 3.0) considered Pt effectors. molecular docking MD simulation suggested candidate Lr28 protein binds more strongly c14094_g1_i1 form stable complex than remaining five. Further functional characterization should prove useful for a better understanding interaction. In turn, this facilitate effector-based leaf resistance breeding wheat.

Language: Английский

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The Role of Epigenetic Communication in the Co-regulation of Holobionts’ Biology: Interspecies Probabilistic Epigenesis

Published: Jan. 1, 2024

Language: Английский

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Recent Technological Advancements for Identifying and Exploiting Novel Sources of Pest and Disease Resistance for Peanut Improvement

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 3071 - 3071

Published: Dec. 23, 2024

Peanut, also known as groundnut (Arachis hypogaea L.), is an important oilseed and food crop globally, contributing significantly to the economy security. However, its productivity often hampered by pests diseases. Traditional breeding methods have been used develop resistant cultivars, but these are time-consuming labor-intensive. Recent technological advancements revolutionized identification of novel resistance sources development peanut cultivars. This review explores latest techniques approaches in for pest disease resistance, focusing on loci their incorporation into using marker-assisted selection (MAS) genomic tools. Next-generation sequencing (NGS) technologies, bioinformatics pipelines, comparative genomics, transcriptomics helped identify a plethora candidate genes involved resistance. lags behind other cereal crops terms phenomics precision genetic functional validation. In conclusion, recent improved efficiency hold great promise developing durable sustainable ultimately benefiting farmers consumers globally.

Language: Английский

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Tae-miR397 Negatively Regulates Wheat Resistance to Blumeria graminis

Plants, Journal Year: 2023, Volume and Issue: 12(17), P. 3096 - 3096

Published: Aug. 29, 2023

MicroRNA (miRNA) plays a crucial role in the interactions between plants and pathogens, identifying disease-related miRNAs could help us understand mechanisms underlying plant disease pathogenesis breed resistant varieties. However, of miRNA wheat defense responses remains largely unexplored. The miR397 family is highly conserved involved development response. Therefore, purpose this study was to investigate function tae-miR397 resistance powdery mildew. expression pattern analysis revealed that higher young leaves than other tissues significantly decreased Bainong207 after Blumeria graminis (Bgt) infection chitin treatment. Additionally, down-regulated by salicylic acid induced under jasmonate overexpression common enhanced wheat's susceptibility mildew seedling adult stages. rate Bgt spore germination mycelial growth transgenic overexpressing faster untransformed wild-type plants. target gene predicted be wound-induced protein (Tae-WIP), investigated. We demonstrated silencing Tae-WIP via barley-stripe-mosaic-virus-induced qRT-PCR indicated regulated immunity controlling pathogenesis-related expressions. Moreover, exhibited more tillers This work suggests negative regulator against has great potential for breeding disease-resistant cultivars.

Language: Английский

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Tae-miR397 negatively regulates wheat resistance to Blumeria graminis

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: June 16, 2023

Abstract MicroRNA (miRNA) plays a crucial role in the interaction of plant-pathogen, which figures out that exploring more disease-associated miRNAs would aid our understanding plant disease pathogenesis. However, miRNA wheat resistance is still largely unknown. In this study, we overexpressed tae-miR397 to investigate its function powdery mildew. The expression pattern analysis revealed was higher young leaves than other tissues, significantly decreased plants after Blumeria graminis ( Bgt ) infection and chitin treatment. Additionally, down-regulated by salicylic acid induced under jasmonate Overexpression enhanced sensitivity rate spore germination mycelial growth on transgenic overexpressing faster untransformed wild type plants. target gene predicted be wound-induced protein (WIP), investigated. We demonstrated silencing WIP via barley stripe mosaic virus-induced susceptibility Furthermore, found regulated immunity controlling pathogenesis-related expressions. Moreover, exhibited tillers These results reveal negative regulator against mildew highlight positively regulating tiller number.

Language: Английский

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