Intermittent fasting applied in combination with astaxanthin alleviates D‐galactose‐induced aging in rats: Comparison in oxidative stress, immune response, and metabolomics DOI
Yong Cao, Xiaojuan Liu, Kaixin Zhao

et al.

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(4)

Published: April 1, 2025

Effective anti-aging strategies involving dietary restriction and antioxidant supplementation are gaining increasing research attention, while the health effects of their combined intervention rarely reported. In this study, for first time, we investigated underlying mechanisms intermittent fasting (IF), astaxanthin (AX), combination in D-galactose-induced aging rats. Our results demonstrated that these three treatments effectively inhibited malondialdehyde levels improved activity endogenous enzymes brain, liver, serum Simultaneously, IF AX had a synergistic effect on recovery brain mitochondrial injury as evidenced by permeability transition pore openness, membrane potential, respiratory chain complex enzyme activity, cortical hippocampal lesions. Notably, significantly increased Immunoglobulin M (55.66 ± 3.23%), immunoglobulin G (34.41 2.65%), IL-2 (23.49 1.78%) compared with model group. Moreover, reduced accumulation pro-inflammatory factor IL-6 (23.06 2.02%), induced more remarkable reduction IL-1β (35.92 3.06%) serum. Considering metabolomics analysis, hypothesized played positive role regulation nervous system, which was associated differential metabolites lysope 16:0, N-Acety-L-tyrosine, L-Alanyl-L-Lysine. This reveals therapy provided efficacy enhancing resistance to oxidative stress, ameliorating dysfunction, restoring immune system. These findings might have significant implications further studies exploration effective therapy.

Language: Английский

Early Synapse-Specific Alterations of Photoreceptor Mitochondria in the EAE Mouse Model of Multiple Sclerosis DOI Creative Commons

Dalia Ibrahim,

Karin Schwarz,

Shweta Suiwal

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 206 - 206

Published: Jan. 30, 2025

Multiple sclerosis (MS) is an inflammatory autoimmune disease of the central nervous system (CNS) linked to many neurological disabilities. The visual frequently impaired in MS. In previous studies, we observed early malfunctions rod photoreceptor ribbon synapses EAE mouse model MS that included alterations synaptic vesicle cycling and disturbances presynaptic Ca2+ homeostasis. Since these events are highly energy-demanding, analyzed whether mitochondria, which play a major role energy metabolism, might be involved at stage. Rod terminals contain single large mitochondrion next ribbon. present study, expression functionally relevant mitochondrial proteins (MIC60, ATP5B, COX1, PINK1, DRP1) by high-resolution qualitative quantitative immunofluorescence microscopy, immunogold electron microscopy Western blot experiments. We decreased mitochondria photoreceptors stage, suggesting dysfunctions important synapse pathology. Interestingly, were strongly compromised EAE, whereas extra-synaptic inner segments remained unchanged, demonstrating functional heterogeneity mitochondria.

Language: Английский

Citations

0
Multi-scale Neural Homeostasis Mechanisms: Insights into Neurodegenerative Diseases and Therapeutic Approaches, Including Exercise DOI Creative Commons
Ya Wang, Xuhui Chen, Cuntai Zhang

et al.

Advanced Exercise and Health Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Intermittent fasting applied in combination with astaxanthin alleviates D‐galactose‐induced aging in rats: Comparison in oxidative stress, immune response, and metabolomics DOI
Yong Cao, Xiaojuan Liu, Kaixin Zhao

et al.

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(4)

Published: April 1, 2025

Effective anti-aging strategies involving dietary restriction and antioxidant supplementation are gaining increasing research attention, while the health effects of their combined intervention rarely reported. In this study, for first time, we investigated underlying mechanisms intermittent fasting (IF), astaxanthin (AX), combination in D-galactose-induced aging rats. Our results demonstrated that these three treatments effectively inhibited malondialdehyde levels improved activity endogenous enzymes brain, liver, serum Simultaneously, IF AX had a synergistic effect on recovery brain mitochondrial injury as evidenced by permeability transition pore openness, membrane potential, respiratory chain complex enzyme activity, cortical hippocampal lesions. Notably, significantly increased Immunoglobulin M (55.66 ± 3.23%), immunoglobulin G (34.41 2.65%), IL-2 (23.49 1.78%) compared with model group. Moreover, reduced accumulation pro-inflammatory factor IL-6 (23.06 2.02%), induced more remarkable reduction IL-1β (35.92 3.06%) serum. Considering metabolomics analysis, hypothesized played positive role regulation nervous system, which was associated differential metabolites lysope 16:0, N-Acety-L-tyrosine, L-Alanyl-L-Lysine. This reveals therapy provided efficacy enhancing resistance to oxidative stress, ameliorating dysfunction, restoring immune system. These findings might have significant implications further studies exploration effective therapy.

Language: Английский

Citations

0