Inhibition of Pyroptosis by Hydroxychloroquine as a Neuroprotective Strategy in Ischemic Stroke DOI Creative Commons

Wenshuo Peng,

Kaiming Guo, Jian Hu

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 12(1), P. ENEURO.0254 - 24.2024

Published: Dec. 18, 2024

Hydroxychloroquine (HCQ), a well-known antimalarial and anti-inflammatory drug, has demonstrated potential neuroprotective effects in ischemic stroke by inhibiting pyroptosis, programmed cell death associated with inflammation. This study investigates the impact of HCQ on pathology using both vivo vitro models. In vivo, C57BL/6 mice subjected to middle cerebral artery occlusion (MCAO) were treated HCQ. Neurological deficits, infarct volume, expression pyroptosis markers evaluated. The results that significantly improved motor function reduced volume MCAO mouse model. vitro, BV2 microglial cells exposed lipopolysaccharide (LPS) oxygen–glucose deprivation (OGD) Western blot immunofluorescence analyses revealed effectively suppressed GSDMD NLRP3 These findings suggest mitigates damage highlighting its as therapeutic agent for stroke. provides novel insights into molecular mechanisms which exerts effects, offering promising new avenue developing safe, cost-effective, widely applicable treatments. modulate neuroinflammatory pathways presents significant advancement therapy, emphasizing importance targeting management broader implications treating conditions.

Language: Английский

Molecular Mimicry Impact of the COVID-19 Pandemic: Sequence Homology Between SARS-CoV-2 and Autoimmune Diseases Epitopes DOI Creative Commons

Pablo J Maldonado-Catala,

Ramkiran Gouripeddi, Naomi Schlesinger

et al.

ImmunoInformatics, Journal Year: 2025, Volume and Issue: unknown, P. 100050 - 100050

Published: March 1, 2025

Molecular mimicry is one mechanism by which an infectious agent may trigger autoimmune disease in a human subject and occurs when foreign- self-peptides contain similar epitopes that activate response susceptible individual. Here, we employ scalable in-silico approach, to identify 861 pairs of known SARS-CoV-2 epitopes, out more than billion possible pairs. These show 1) sequence homology disorder 2) empirical binding data predict they bind the same major histocompatibility complex (MHC) molecule 3) exhibit high immunogenicity. Analysis these epitope reveals association between disorders, such as type 1 diabetes, uveitis, ankylosing spondylitis, infection. associations are consistent with those reported literature from analysis clinical records.

Language: Английский

Citations

1
Research Hotspots and Frontiers of Alzheimer’s Disease and Gut Microbiota: A Knowledge Mapping and Text Mining Analysis DOI

Youao Zhang,

Zixuan Jia,

Jieyan Wang

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(11), P. 9369 - 9382

Published: April 18, 2024

Language: Английский

Citations

5
Deciphering the IgG Idiotype Network Through Proteomic Analysis of Potential Targets in SARS‐CoV‐2‐Induced Immune Responses DOI Open Access
Nicolle Rakanidis Machado, Beatriz Oliveira Fagundes,

Lais Alves do Nascimento

et al.

Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

ABSTRACT The association between COVID‐19 and autoimmune diseases has gained increasing recognition, yet the specific targets of SARS‐CoV‐2‐induced IgG are currently in focus for several studies. This study aims to explore proteomic these antibodies their potential role autoimmunity. We utilised a human proteome microarray encompassing 23 736 unique proteins, including isoform variants fragments, as catalogued by Human Protein Atlas. Serum samples were analysed from four groups: healthy controls (N‐exp HC), individuals vaccinated with protein‐based vaccines (N‐Cov Vac) patients moderate or severe (COVID‐Mod COVID‐Sev). evaluation revealed recognise multiple proteins. Key included interferon alpha (IFN‐α), tumour growth factor beta (TGF‐β), interleukin 1 (IL‐1), CXCL16, TGF‐β receptors, CD34, CD47 BCL2. also targeted proteins genes overexpressed various immune cells, such CD4+ CD8+ T γδ B dendritic cells NK cells. Reactivity was observed specifically expressed organs, brain, liver, lungs heart. Targeting patterns differed controls, some showing differential recognition versus cases. Furthermore, we evaluated protein–protein interaction network (PPIN) all minimal structural homology co‐expression among almost no relation SARS‐CoV‐2 system reactome. results suggest that profile autoantibodies is associated disease severity. In contrast, protein‐vaccinated exhibited similar non‐exposed suggesting autoreactive linked active infection. These findings reveal complex idiotypes capable targeting not merely through simple cross‐recognition homologous highlights need further investigations determine whether they may influence pathophysiology its clinical outcomes.

Language: Английский

Citations

0
The status and hotspot analysis of research on extracellular vesicles and osteoarthritis: a bibliometric analysis DOI Creative Commons

W. Zhang,

Wen Xiang,

Lin Yi

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

Degenerative joint disease, known as osteoarthritis (OA), is characterized by pain, swelling, and decreased mobility. The illness has a major negative influence on patients' quality of life common around the world, especially among older people. Nevertheless, there are insufficient possibilities for early diagnosis therapy. Extracellular vesicles, or EVs, control immune response, tissue healing, cellular communication. This work offers bibliometric representation areas focus correlations between extracellular vesicles osteoarthritis. We searched in publications Web Science Core Collection (WoSCC) database. Bibliometrics, an R package, CiteSpace 6.1. R2, VOSviewer 1.6.17 were used to perform analyses concentration fields, trends, relevant factors. 944 papers from 59 nations published; countries that contributed most field China, USA, Italy. Professors Laura Enrico top contributors. Sichuan University, Istituto Ortopedico Galeazzi, Shanghai Jiao Tong University three universities. International Journal Molecular Sciences excellent publication. Exosome, expression, knee osteoarthritis, vesicle, mesenchymal stem cell, inflammation often occurring keywords. These results suggest interest future research EVs OA. trend suggests volume literature OA will continue rise, with more being published future. study helps scholars understand current hotspots may inspire research.

Language: Английский

Citations

0
Research trends in essential thrombocythemia from 2001 to 2024: a bibliometric analysis DOI Creative Commons
Yiming Pan, Lingyan Chen,

Qun Jiang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 15, 2025

Language: Английский

Citations

0
Relationship between gut microbiota and multiple sclerosis: A comprehensive bibliometric and visual analysis of global research trends and therapeutics DOI

Aishwarya Bhattacharya,

Kushagra Agarwal,

Nirmal Kumar Ganguly

et al.

Next research., Journal Year: 2025, Volume and Issue: unknown, P. 100441 - 100441

Published: May 1, 2025

Language: Английский

Citations

0
Current status and future directions of nanovaccine for cancer: a bibliometric analysis during 2004-2023 DOI Creative Commons

Yuhui Hou,

Yue Li,

Youao Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 29, 2024

Nanovaccine treatment is an exciting area of research in immunology and personalized medicine, holding great promise for enhancing immune responses targeting specific diseases. Their small size allows efficient uptake by cells, leading to robust activation. They can incorporate immune-stimulating molecules boost vaccine efficacy. Therefore, nanovaccine be target tumor-specific antigens, activating the system against cancer cells. Currently, there have been ample evidence showing effectiveness potential as a cancer. However, was rare bibliometric analysis Here we performed visual published studies related cancer, providing trend future development nanovaccine.

Language: Английский

Citations

3
Inhibition of Pyroptosis by Hydroxychloroquine as a Neuroprotective Strategy in Ischemic Stroke DOI Creative Commons

Wenshuo Peng,

Kaiming Guo, Jian Hu

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 12(1), P. ENEURO.0254 - 24.2024

Published: Dec. 18, 2024

Hydroxychloroquine (HCQ), a well-known antimalarial and anti-inflammatory drug, has demonstrated potential neuroprotective effects in ischemic stroke by inhibiting pyroptosis, programmed cell death associated with inflammation. This study investigates the impact of HCQ on pathology using both vivo vitro models. In vivo, C57BL/6 mice subjected to middle cerebral artery occlusion (MCAO) were treated HCQ. Neurological deficits, infarct volume, expression pyroptosis markers evaluated. The results that significantly improved motor function reduced volume MCAO mouse model. vitro, BV2 microglial cells exposed lipopolysaccharide (LPS) oxygen–glucose deprivation (OGD) Western blot immunofluorescence analyses revealed effectively suppressed GSDMD NLRP3 These findings suggest mitigates damage highlighting its as therapeutic agent for stroke. provides novel insights into molecular mechanisms which exerts effects, offering promising new avenue developing safe, cost-effective, widely applicable treatments. modulate neuroinflammatory pathways presents significant advancement therapy, emphasizing importance targeting management broader implications treating conditions.

Language: Английский

Citations

0