Reduction of Staphylococcus aureus biofilms by lactic acid bacteria isolated from fermented transitional milk DOI
Eduardo Robson Duarte, Hugo Calixto Fonseca, Fernanda Guimarães

et al.

New Zealand Journal of Agricultural Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Nov. 20, 2024

This study aimed to evaluate the inhibitory effect of lactic acid bacteria (LAB) isolated from fermented transition milk against Staphylococcus aureus cows with mastitis adhered teat cup rubber and stainless steel surfaces cows. Transition milk, collected multiparous Holstein cows, was for 33 days. LAB were based on their different colony morphologies MRS agar identified using MALDI-TOF MS analysis. The antagonistic activity determined by double-layer diffusion assay inhibition S. biofilms supernatant. Among isolates evaluated, Lacticaseibacillus paracasei, casei, Lactobacillus rhamnosus, Enterococcus faecalis, faecium identified. Out fifteen isolates, nine exhibited zones ranging 12.53 25.83 mm two strains aureus. Lower concentrations both coupons detected after 20 min contact supernatants three selected when compared control. These findings suggest that metabolites produced could potentially be used as an alternative method inhibit control antimicrobial-resistant strains, well reduce biofilm formation.

Language: Английский

Investigating the Clogging Mechanism in Membrane Discharge Irrigation Systems Using Reclaimed Water: A Comparative Case Study of Trace Quantity Irrigation and Moistube Irrigation DOI Open Access
Ping Xu, Yuanzhe Zhao, Yumin Ou

et al.

Irrigation and Drainage, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

ABSTRACT Trace quantity irrigation (TQI) and moistube (MTI) are membrane discharge (MDI) systems designed to minimize water use prevent clogging. When laboratory‐prepared reclaimed (similar the China Class I‐B standard) was used, emitter clogging (EC) occurred faster in TQI, escalating within 216 h, compared 312 h for MTI. Analysis of dry weight (DW), extracellular polysaccharides (PSs) proteins (PNs) different parts pipes revealed that PS PN contents contributed EC, with end part being most affected. High‐throughput sequencing identified Proteobacteria as a key factor clogging, Aquabacterium dominant TQI Pseudomonas MTI, whereas Methylophilus common both, suggesting aerobic anaerobic alternations exist pipe. Computational fluid dynamic (CFD) simulations indicated had flow velocity greater shear, leading DWs MTI (1.79–2.27 times higher than TQI) but similar (1.06–1.47 times) (0.87–1.03 levels. To manage MDI should apply chlorination pressure flushing before intensifies, adjusting duration according quality.

Language: Английский

Citations

0
Biodeterioration Study of Cementitious Materials During Sewage Treatment Processes DOI Open Access
Nedson T. Kashaija,

Viktória Gável,

Gabriel Iklaga

et al.

Water, Journal Year: 2025, Volume and Issue: 17(3), P. 459 - 459

Published: Feb. 6, 2025

The relationship between microbial communities and mineralogical/mechanical changes was studied regarding the biodeterioration of Portland cement (PC) calcium sulfoaluminate (CSAC) in a wastewater treatment plant. An situ experiment conducted by submerging 12 independent PC CSAC specimens sand-trap structure for 10, 30, 75, 150, 240 days. microbiological analyses 16S rRNA genes bacteria Archaea from biofilms geochemical analysis were performed on specimens. results showed that while there characteristic over time, few effects. dominant identified belonged to classes Gammaproteobacteria (8.4–32.4%), Bacilli (1.6–21.6%), Clostridia (4–15.4%), Bacteroidia (2–18.8%), Desulfovibronia (0.5–19%), Campylobacteria (0.4–26.8%), Actinobacteria (1.8–12.8%). overall relative abundance linked processes increased more than 50% total bacterial after 75 days sewage exposure found be strongly correlated with several deterioration parameters (e.g., mass loss, calcite ettringite minerals), whereas no significant correlation revealed these genera characteristics.

Language: Английский

Citations

0
Potential of Bacillus halotolerans in Mitigating Biotic and Abiotic Stresses: A Comprehensive Review DOI Creative Commons
Pelias Rafanomezantsoa, Abbas El‐Hasan, Ralf T. Voegele

et al.

Stresses, Journal Year: 2025, Volume and Issue: 5(2), P. 24 - 24

Published: March 25, 2025

Bacillus halotolerans, a halophilic bacterial species of the genus Bacillus, is emerging as biological control agent with immense potential for sustainable agriculture, particularly in extreme conditions and environmental rehabilitation. This review summarizes current state research on B. emphasizing its diverse applications biocontrol plant pathogens, growth promotion under salinity stress, nematode management, bioremediation. halotolerans utilizes several mechanisms such production siderophores phytohormones, secretion exopolysaccharides, release antifungal nematicidal compounds, which allows it to mitigate both abiotic biotic stresses various crops, including wheat, rice, date palm, tomato, others. In addition, genomic metabolomic analyses have revealed secondary metabolite that improves antagonistic growth-promoting traits. Despite significant progress, challenges remain translating laboratory results into field applications. Future should focus formulating effective bioinoculants trials maximize practical utility agriculture resilience.

Language: Английский

Citations

0
Modes of Action of Biocontrol Agents from Rhizosphere to Phyllosphere Signaling DOI
Bakhodir Mamarasulov, Kakhramon Davranov,

T. G. Gulyamova

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Chemical Composition and Antibacterial Effect of Clove and Thyme Essential Oils on Growth Inhibition and Biofilm Formation of Arcobacter spp. and Other Bacteria DOI Creative Commons
Leona Hofmeisterová, Tomáš Bajer, Maciej Walczak

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1232 - 1232

Published: Dec. 20, 2024

Background: In recent years, significant resistance of microorganisms to antibiotics has been observed. A biofilm is a structure that significantly aids the survival microbial population and also affects its resistance. Methods: Thyme clove essential oils (EOs) were subjected chemical analysis using gas chromatography coupled mass spectrometry (GC-MS) with flame ionization detector (GC-FID). Furthermore, antimicrobial effect these EOs was tested in both liquid vapor phases volatilization method. The on growth parameters monitored an RTS-8 bioreactor. However, formation commonly occurring bacteria pathogenic potential monitored, but for less described yet clinically important strains Arcobacter spp. Results: total, 37 28 compounds identified thyme EO samples, respectively. most common terpenes derivatives phenolic substances. Both exhibited activity and/or phase against at least some strains. determined oil range 32–1024 µg/mL 512–1024 phase, results are supported by similar conclusions from monitoring curves RTS differed between Biofilm Pseudomonas aeruginosa completely suppressed environment concentration 1024 µg/mL. On other hand, increased found, e.g., low (1–32 µg/mL). Conclusions: natural matrices as antimicrobials or preservatives evident. formation, especially strains, first time.

Language: Английский

Citations

1
Synergistic Antimicrobial Action of Polymerized Indole-Based Nanocomposites with NfAg on Chemically Reformed Multiple-Layered Carbon Nanotubes DOI

Hamit Ismaili,

Syed Khalid Mustafa, Noha Omer

et al.

BioNanoScience, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Language: Английский

Citations

0
Peptide-Based Biomaterials for Combatting Infections and Improving Drug Delivery DOI Creative Commons
Lucia Lombardi, Jiaxu Li, Daryl R. Williams

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(11), P. 1468 - 1468

Published: Nov. 18, 2024

This review explores the potential of peptide-based biomaterials to enhance biomedical applications through self-assembly, biological responsiveness, and selective targeting. Peptides are presented as versatile agents for antimicrobial activity drug delivery, with recent approaches incorporating peptides into self-assembling systems improve effectiveness reduce resistance. The also covers nanocarriers cancer highlighting their improved stability, targeted reduced side effects. focus this work is on bioactive properties peptides, particularly in infection control rather than structural design or material characteristics. Additionally, it examines role peptidomimetics broadening biomaterial enhancing resistance enzymatic degradation. Finally, discusses commercial prospects challenges translating peptide clinical applications.

Language: Английский

Citations

0
Reduction of Staphylococcus aureus biofilms by lactic acid bacteria isolated from fermented transitional milk DOI
Eduardo Robson Duarte, Hugo Calixto Fonseca, Fernanda Guimarães

et al.

New Zealand Journal of Agricultural Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Nov. 20, 2024

This study aimed to evaluate the inhibitory effect of lactic acid bacteria (LAB) isolated from fermented transition milk against Staphylococcus aureus cows with mastitis adhered teat cup rubber and stainless steel surfaces cows. Transition milk, collected multiparous Holstein cows, was for 33 days. LAB were based on their different colony morphologies MRS agar identified using MALDI-TOF MS analysis. The antagonistic activity determined by double-layer diffusion assay inhibition S. biofilms supernatant. Among isolates evaluated, Lacticaseibacillus paracasei, casei, Lactobacillus rhamnosus, Enterococcus faecalis, faecium identified. Out fifteen isolates, nine exhibited zones ranging 12.53 25.83 mm two strains aureus. Lower concentrations both coupons detected after 20 min contact supernatants three selected when compared control. These findings suggest that metabolites produced could potentially be used as an alternative method inhibit control antimicrobial-resistant strains, well reduce biofilm formation.

Language: Английский

Citations

0