Transient isotachophoresis–Capillary zone electrophoresis–Mass spectrometry method with off-line microscale solid phase extraction pretreatment for quantitation of intact low molecular mass proteins in various biological fluids DOI Creative Commons
Martina Opetová, Radovan Tomašovský, Peter Mikuš

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1718, P. 464697 - 464697

Published: Feb. 3, 2024

Quantification of proteins is still predominantly done by the traditional bottom-up approach. Targeting intact in complex biological matrices connected with multiple challenges during sample pretreatment, separation, and detection step analytical workflow. In this work, we focused on development an on-line hyphenated capillary zone electrophoresis-mass spectrometry method employing off-line microscale solid-phase extraction based hydrophilic lipophilic balance (HLB) sorbent as a pretreatment for analysis low molecular mass (<20 kDa) spiked various fluids (human serum, plasma, urine, saliva). A detailed optimization process involved selection suitable surface, background electrolyte (BGE), comparison two in-capillary preconcentration methods, namely transient isotachophoresis (tITP) dynamic pH junction (DPJ), to enhance sensitivity method. Optimum separation analytes was achieved using uncoated bare fused silica 500 mM formic acid (pH 1.96) + 5% (v/v) acetonitrile BGE. tITP utilized optimum technique, achieving 19- 127-fold increase signal intensity when 200 ammonium formate (adjusted 4.00) leading BGE terminating electrolyte. Off-line eluate treatment procedures evaluated ensure compatibility selected preconcentration, process. Achieved recoveries were range 76-100% 12-54% 21-106% 25-98% saliva evaporated reconstituted solution achieve The validated across different matrices, offering good linearity, accuracy, precision, making it proteomic studies (e.g., therapeutic drug monitoring, biomarker research) samples.

Language: Английский

Recent developments in sample preparation techniques combined with high-performance liquid chromatography: A critical review DOI Creative Commons
Abu B. Kanu

Journal of Chromatography A, Journal Year: 2021, Volume and Issue: 1654, P. 462444 - 462444

Published: July 30, 2021

Language: Английский

Citations

199
Recent advances in proteomics and metabolomics in plants DOI Creative Commons
Shijuan Yan, Ruchika Bhawal, Zhibin Yin

et al.

Molecular Horticulture, Journal Year: 2022, Volume and Issue: 2(1)

Published: July 23, 2022

Over the past decade, systems biology and plant-omics have increasingly become main stream in plant research. New developments mass spectrometry bioinformatics tools, methodological schema to integrate multi-omics data leveraged recent advances proteomics metabolomics. These progresses are driving a rapid evolution field of research, greatly facilitating our understanding mechanistic aspects metabolisms interactions plants with their external environment. Here, we review MS-based metabolomics tools workflows special focus on applications research using several case studies related stress response, gene/protein function characterization, metabolic signaling pathways exploration, natural product discovery. We also present projection concerning future perspectives development including challenges for system biology. This is intended provide readers an overview how advanced MS technology, integrated application can be used advance

Language: Английский

Citations

72
Metal–Organic Frameworks as Key Materials for Solid-Phase Microextraction Devices—A Review DOI Creative Commons
Adrián Gutiérrez‐Serpa, Idaira Pacheco‐Fernández, Jorge Pasán

et al.

Separations, Journal Year: 2019, Volume and Issue: 6(4), P. 47 - 47

Published: Oct. 2, 2019

Metal–organic frameworks (MOFs) have attracted recently considerable attention in analytical sample preparation, particularly when used as novel sorbent materials solid-phase microextraction (SPME). MOFs are highly ordered porous crystalline structures, full of cavities. They formed by inorganic centers (metal ion atoms or metal clusters) and organic linkers connected covalent coordination bonds. Depending on the ratio such precursors synthetic conditions, characteristics resulting MOF vary significantly, thus drifting into a countless number interesting with unique properties. Among astonishing features MOFs, their high chemical thermal stability, easy tuneability, simple synthesis, impressive surface area (which is highest known), most attractive that makes them outstanding SPME. This review offers an overview current state use different SPME configurations, all cases covering extraction devices coated (or incorporating) particular emphases preparation.

Language: Английский

Citations

101
The recent advances in magnetic sorbents and their applications DOI
Mohammad Faraji, Mahboube Shirani, Hamid Rashidi Nodeh

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 141, P. 116302 - 116302

Published: April 21, 2021

Language: Английский

Citations

99
Recent Materials Developed for Dispersive Solid Phase Extraction DOI Creative Commons
Piotr Ścigalski, Przemysław Kosobucki

Molecules, Journal Year: 2020, Volume and Issue: 25(21), P. 4869 - 4869

Published: Oct. 22, 2020

Solid phase extraction (SPE) is an analytical procedure developed with the purpose of separating a target analyte from complex sample matrix prior to quantitative or qualitative determination. The such treatment twofold: elimination constituents that could interfere detection process even damage equipment as well enriching in so it readily available for detection. Dispersive solid (dSPE) recent development standard SPE technique attracting growing attention due its remarkable simplicity, short time and low requirement solvent expenditure, accompanied by high effectiveness wide applicability. This review aims thoroughly survey recently conducted studies focusing on methods utilizing novel, interesting nanomaterials dSPE sorbents, known materials have been only successfully applied techniques, evaluate their performance suitability based comparison previously reported procedures.

Language: Английский

Citations

96
Miniaturized Solid Phase Extraction techniques for different kind of pollutants analysis: State of the art and future perspectives – PART 1 DOI
Justyna Płotka‐Wasylka, Natalia Jatkowska, Monika Paszkiewicz

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 162, P. 117034 - 117034

Published: March 28, 2023

Language: Английский

Citations

38
Automated and semi-automated packed sorbent solid phase (micro) extraction methods for extraction of organic and inorganic pollutants DOI

Mahsa Samadifar,

Yadollah Yamini, Mohammad Mahdi Khataei

et al.

Journal of Chromatography A, Journal Year: 2023, Volume and Issue: 1706, P. 464227 - 464227

Published: July 20, 2023

Language: Английский

Citations

27
Sample preparation strategies for mass spectrometry analysis in human exposome research: Current status and future perspectives DOI Creative Commons
Yunyun Gu, Jesse T. Peach, Benedikt Warth

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 166, P. 117151 - 117151

Published: June 24, 2023

The exposome is the measure of total environmental exposures and has a significant impact on human health outcomes. Exposomics research typically examines complex biological samples assesses 100s to 1,000s small molecules associated with chemical exposure events. To acquire robust comprehensive datasets encompassing broad range multi-class biomarkers exposures, high-resolution tandem mass spectrometry have emerged as preferred analytical tools in field. Due matrix effects interferences together presence very low abundance analytes, sensitivity reproducibility plagued exposomic studies. This especially true for large sample sets where adequate robustness frequently lacking. A possible solution this problem development non-discriminatory preparation methods which can reduce while increasing reproducibility. However, techniques intrinsically result discrete loss analytes leading outstanding challenge finding proper balance. Therefore, approaches decrease preserving coverage need be developed. In review, procedures currently applied related fields are summarized critically evaluated. includes comparison advantages limitations between different such solid-phase extraction, liquid QuEChERS, filters dilute shoot chromatography-mass analysis focus. Based evaluation, recommendations promoting balanced strategies future applications provided.

Language: Английский

Citations

25
A guide to the use of bioassays in exploration of natural resources DOI Creative Commons
Jerica Sabotič,

Engin Bayram,

‪David Ezra‬‏

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 71, P. 108307 - 108307

Published: Jan. 5, 2024

Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality critical for optimal bioprospecting. They used both in early stages of compounds isolation/purification/identification, later evaluate safety efficacy. In this review, we provide a comprehensive overview most common bioassays discovery development new bioactive with focus on marine bioresources. We present list practical considerations selecting appropriate discuss detail typically explore antimicrobial, antibiofilm, cytotoxic, antiviral, antioxidant, anti-ageing potential. The concept control bioassay validation introduced, followed by considerations, which advancing higher stage development. conclude providing an application-oriented view focused pharmaceuticals, food supplements, cosmetics, industrial pipelines where currently known products hold highlight importance gaining reliable results, as these serve starting point application-based further testing, well consideration regulatory authorities.

Language: Английский

Citations

9
Development and Validation of a New Micro‐Solid‐Phase Extraction Cartridge Based On MIL‐101(Cr) in the Determination of Multiclass Pesticides in Fruit Juice Samples DOI
Abdollah Omidi, Kourosh Tabar Heydar, Seyyed Hamid Ahmadi

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 1, 2025

ABSTRACT In this research, the metal–organic framework MIL‐101(Cr) and porous Teflon–fluorocarbon resin (ChromosorbT, 40–60 mesh) are physically mixed used as a novel micro‐solid‐phase extraction (µ‐SPE) cartridge sorbent for efficient enrichment of nine multiclass pesticides from fruit juice samples, followed by gas chromatography–mass spectrometry. Several experimental parameters, including effect weight, ratio ChromosorbT weight to sorbent, flow rate sample solution, volume type elution solvent, were investigated optimized. The limits detection (LODs, S/N = 3) quantification (LOQs, 10) all analytes obtained proposed method in ranges 0.1–1.5 µg/L 0.5–5 µg/L, respectively. intra‐ interday ( n precisions at spiked levels 5 10 0.1%–11.5% 0.1%–7.2%, addition, two above‐mentioned concentrations, achieved recovery percentages 60.7–102.9 when applied determine real samples spiked. According advantageous findings, was simple, sensitive, fast, had great potential extracting analyzing pesticide residues samples.

Language: Английский

Citations

1