Transient isotachophoresis–Capillary zone electrophoresis–Mass spectrometry method with off-line microscale solid phase extraction pretreatment for quantitation of intact low molecular mass proteins in various biological fluids DOI Creative Commons
Martina Opetová, Radovan Tomašovský, Peter Mikuš

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1718, P. 464697 - 464697

Published: Feb. 3, 2024

Quantification of proteins is still predominantly done by the traditional bottom-up approach. Targeting intact in complex biological matrices connected with multiple challenges during sample pretreatment, separation, and detection step analytical workflow. In this work, we focused on development an on-line hyphenated capillary zone electrophoresis-mass spectrometry method employing off-line microscale solid-phase extraction based hydrophilic lipophilic balance (HLB) sorbent as a pretreatment for analysis low molecular mass (<20 kDa) spiked various fluids (human serum, plasma, urine, saliva). A detailed optimization process involved selection suitable surface, background electrolyte (BGE), comparison two in-capillary preconcentration methods, namely transient isotachophoresis (tITP) dynamic pH junction (DPJ), to enhance sensitivity method. Optimum separation analytes was achieved using uncoated bare fused silica 500 mM formic acid (pH 1.96) + 5% (v/v) acetonitrile BGE. tITP utilized optimum technique, achieving 19- 127-fold increase signal intensity when 200 ammonium formate (adjusted 4.00) leading BGE terminating electrolyte. Off-line eluate treatment procedures evaluated ensure compatibility selected preconcentration, process. Achieved recoveries were range 76-100% 12-54% 21-106% 25-98% saliva evaporated reconstituted solution achieve The validated across different matrices, offering good linearity, accuracy, precision, making it proteomic studies (e.g., therapeutic drug monitoring, biomarker research) samples.

Language: Английский

Pharmaceuticals, pesticides and metals/metalloids in Lake Guaíba in Southern Brazil: Spatial and temporal evaluation and a chemometrics approach DOI
Maurício Perin, Alexsandro Dallegrave,

Lucas Suchecki Barnet

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 793, P. 148561 - 148561

Published: June 22, 2021

Language: Английский

Citations

28
Amylodextrin hydrogel as a green sorbent for pipette-tip micro-solid phase extraction followed by ion mobility spectrometry for analysis of triazole fungicides in environmental water samples DOI

Nasim Rezaie,

Saeed Nojavan, Majid Behpour

et al.

Microchemical Journal, Journal Year: 2022, Volume and Issue: 181, P. 107803 - 107803

Published: July 21, 2022

Language: Английский

Citations

20
Broad-range extraction of highly polar to non-polar organic contaminants for inclusive target analysis and suspect screening of environmental samples DOI
Lahiruni M. Halwatura, Diana S. Aga

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 893, P. 164707 - 164707

Published: June 9, 2023

Language: Английский

Citations

12
Dendrimer Coated Silica as a Sorbent for Dispersive Solid-Phase Extraction of Select Non-Steroidal Anti-Inflammatory Drugs from Water DOI Creative Commons
Piotr Ścigalski, Przemysław Kosobucki

Molecules, Journal Year: 2024, Volume and Issue: 29(2), P. 380 - 380

Published: Jan. 12, 2024

Non-steroidal anti-inflammatory drugs (NSAIDs) have been recognized as a potentially serious threat to the natural environment. NSAIDs are popular painkillers, and main pathway for them reach water is via discharge from wastewater sewage treatment plants. In order monitor contamination caused by these drugs, well their impact on environment, new material based Silica Gel 60, functionalized with dendrimeric copolymer of methylamine 1,4-butanediol diglycidyl ether (named MA-BDDE), was prepared. Initial physicochemical characterization MA-BDDE carried out using ATR FT-IR spectroscopy solid-state carbon-13 NMR spectroscopy. Its effectiveness at NSAID extraction evaluated application five select in dispersive solid-phase (dSPE): aspirin, ketoprofen, naproxen, diclofenac ibuprofen. This followed simultaneous determination HPLC-UV/Vis system demonstrating good sensitivity, limits detection values within 63-265 ng mL

Language: Английский

Citations

4
Transient isotachophoresis–Capillary zone electrophoresis–Mass spectrometry method with off-line microscale solid phase extraction pretreatment for quantitation of intact low molecular mass proteins in various biological fluids DOI Creative Commons
Martina Opetová, Radovan Tomašovský, Peter Mikuš

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1718, P. 464697 - 464697

Published: Feb. 3, 2024

Quantification of proteins is still predominantly done by the traditional bottom-up approach. Targeting intact in complex biological matrices connected with multiple challenges during sample pretreatment, separation, and detection step analytical workflow. In this work, we focused on development an on-line hyphenated capillary zone electrophoresis-mass spectrometry method employing off-line microscale solid-phase extraction based hydrophilic lipophilic balance (HLB) sorbent as a pretreatment for analysis low molecular mass (<20 kDa) spiked various fluids (human serum, plasma, urine, saliva). A detailed optimization process involved selection suitable surface, background electrolyte (BGE), comparison two in-capillary preconcentration methods, namely transient isotachophoresis (tITP) dynamic pH junction (DPJ), to enhance sensitivity method. Optimum separation analytes was achieved using uncoated bare fused silica 500 mM formic acid (pH 1.96) + 5% (v/v) acetonitrile BGE. tITP utilized optimum technique, achieving 19- 127-fold increase signal intensity when 200 ammonium formate (adjusted 4.00) leading BGE terminating electrolyte. Off-line eluate treatment procedures evaluated ensure compatibility selected preconcentration, process. Achieved recoveries were range 76-100% 12-54% 21-106% 25-98% saliva evaporated reconstituted solution achieve The validated across different matrices, offering good linearity, accuracy, precision, making it proteomic studies (e.g., therapeutic drug monitoring, biomarker research) samples.

Language: Английский

Citations

4