Large-arena field cage releases of a candidate classical biological control agent for spotted wing drosophila suggest low risk to non-target species

Journal of Pest Science, Journal Year: 2022, Volume and Issue: 95(3), P. 1057 - 1065

Published: March 4, 2022

Abstract Classical biological control, i.e., the introduction of natural enemies from an invasive pest’s area origin, has been proposed repeatedly to control spotted wing drosophila Drosophila suzukii in Americas and Europe. Results surveys Asia laboratory experiments suggest parasitoid G1 Ganaspis cf. brasiliensis as a suitable agent. To study host specificity under semi-field conditions, we conducted large-arena field cage releases. Parasitoids were released into cages at three dates August 2021 two regions Switzerland. Released parasitoids had choice parasitize either D. larvae fresh fruits (blueberries or elderberries) non-target native species melanogaster decomposing fruits. The results unequivocal that apparent parasitism feeding was on average 15%, whereas only one emerged (0.02% parasitism). Thus, support findings previous G. is highly specific closely related very rare. Because its invaded range, can attack develop undamaged fruits, conclude open releases should not pose significant risks non-targets.

Language: Английский

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Prioritization of invasive alien species with the potential to threaten agriculture and biodiversity in Kenya through horizon scanning

Biological Invasions, Journal Year: 2022, Volume and Issue: 24(9), P. 2933 - 2949

Published: May 31, 2022

Abstract Invasive alien species (IAS) rank among the most significant drivers of extinction and ecosystem degradation resulting in impacts on socio-economic development. The recent exponential spread IAS Africa is attributed to poor border biosecurity due porous borders that have failed prevent initial introductions. In addition, countries lack adequate information about potential invasions limited capacity reduce risk invasions. Horizon scanning an approach prioritises risks through rapid assessments. A group 28 subject matter experts used adapted methodology assess 1700 a 5-point scale for likelihood entry establishment, impact, impact biodiversity. individual scores were combined according their overall country. Confidence was recorded 3-point scale. This resulted priority list 120 (70 arthropods, 9 nematodes, 15 bacteria, 19 fungi/chromist, 1 viroid, 6 viruses). Options mitigation such as full pest analysis detection surveys suggested prioritised while which no immediate action suggested, added plant health register recommendation made regularly monitor change risk. By prioritising risks, horizon guides resource allocation interventions are likely very useful National Plant Protection Organisations other relevant stakeholders.

Language: Английский

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Evaluation of a Push–Pull Strategy for Spotted-Wing Drosophila Management in Highbush Blueberry

Insects, Journal Year: 2024, Volume and Issue: 15(1), P. 47 - 47

Published: Jan. 10, 2024

We evaluated a novel push-pull control strategy for protecting highbush blueberry,

Language: Английский

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A generalized risk assessment index for forecasting insect population under the effect of temperature

Journal of Thermal Biology, Journal Year: 2024, Volume and Issue: 122, P. 103886 - 103886

Published: May 1, 2024

Life history traits have been studied under various environmental factors, but the ability to combine them into a simple function assess pest response climate is still lacking complete understanding. This study proposed risk index derived by combining development, mortality, and fertility rates from stage-structured dynamic mathematical model. The first part presents theoretical framework behind index. second of concerned with application in two case studies major economic pest: brown planthopper (Nilaparvata lugens) spotted wing drosophila (Drosophila suzukii), pests rice crops soft fruits, respectively. calculations provided single composed main thermal biodemographic rates. has threshold value that determines possibility population increase as temperature. tests carried out on species showed capability describe range favourable conditions. With this approach, we were able identify areas where are tolerant climatic conditions project geospatial map. background developed here tool for understanding biogeography Nilaparvata lugens Drosophila suzukii. It flexible enough deal mathematically (N. complex (D. Suzukii) crop insect pests. produces biologically sound indices behave like performance curves. These results also provide reasonable basis addressing challenge management context seasonal weather variations change. may help improve monitoring design strategies limit spread invaded areas, some non-invaded be suitable develop.

Language: Английский

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Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(13), P. 6724 - 6724

Published: June 23, 2021

The spotted-wing Drosophila (Drosophila suzukii Matsumura) is native to eastern Asia, but has become a global threat fruit production. In recent years, CRISPR/Cas9 targeting was established in this species allowing for functional genomic and genetic control studies. Here, we report the generation characterization of Cas9-expressing strains D. suzukii. Five independent transgenic lines were generated using piggyBac construct containing EGFP fluorescent marker gene Cas9 under melanogaster heat shock protein 70 promoter 3’UTR. Heat-shock (HS) treated embryos analyzed by reverse transcriptase PCR, revealing strong inducibility expression. By injecting gRNA into one selected line, 50.0% G0 flies showed mosaic loss-of-fluorescence phenotype, 45.5% produced G1 mutants without HS. Such somatic germline mutagenesis rates increased 95.4% 85.7%, respectively, applying Parental receiving HS resulted high inheritance mutation (92%) their progeny. Additionally, endogenous yellow led lack pigmentation male lethality. We discuss potential use these efficient temperature-dependent studies

Language: Английский

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First record of the agricultural pest Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) in Algeria

EPPO Bulletin, Journal Year: 2022, Volume and Issue: 52(2), P. 471 - 478

Published: Aug. 1, 2022

Abstract The spotted wing drosophila Drosophila suzukii (Matsumura, 1931) could potentially become a serious invasive pest of fruit crops in the North Africa coming years, leading to substantial economic losses. This is reported for first time Algeria. It was detected M'sila north‐east country within an pomegranate agroecosystem near salt lake Chott Zahrez Echergui. Trapping conducted from 21 March 2018 23 January 2019 two orchards. prevalence (flies/trap/day) and population dynamics D. other flies ( Zaprionus indianus melanogaster ) trapped same orchards are reported. Low numbers these three fly species were found trap samples. At present, ecological impact on poorly known Africa.

Language: Английский

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A Fuzzy-Based Model to Predict the Spatio-Temporal Performance of the Dolichogenidea gelechiidivoris Natural Enemy against Tuta absoluta under Climate Change

Biology, Journal Year: 2022, Volume and Issue: 11(9), P. 1280 - 1280

Published: Aug. 28, 2022

The South American tomato pinworm, Tuta absoluta, causes up to 100% crop losses. As absoluta is non-native African agroecologies and lacks efficient resident natural enemies, the microgastrine koinobiont solitary oligophagous larval endoparasitoid, Dolichogenidea gelechiidivoris (Marsh) (Syn.: Apanteles Marsh) (Hymenoptera: Braconidae) was released for classical biological control. This study elucidates current future spatio-temporal performance of D. against T. in cropping systems using a fuzzy logic modelling approach. Specifically, considers presence host crop, as well parasitoid reproductive capacity, key variables. Results show that algorithm predicted (in terms net rate (R0)), with low root mean square error (RMSE) value (<0.90) considerably high R2 coefficient (=0.98), accurately predicting over time space. Under climatic scenario, perform all regions throughout year, except coastal region. projected improve year. Overall, model sheds light on varying across different Kenya, seasons, under both scenarios.

Language: Английский

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Hanseniaspora uvarum Attracts Drosophila suzukii (Diptera: Drosophilidae) With High Specificity

Journal of Economic Entomology, Journal Year: 2022, Volume and Issue: 115(4), P. 999 - 1007

Published: Feb. 19, 2022

Since the early phase of intercontinental dispersal Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), fermentation baits have been used for monitoring. Self-made lures and commercial products are often based on wine vinegar. From an ecological perspective, formulation these is expected to target especially vinegar flies associated with overripe fruit, such as melanogaster (Meigen) Drosophilidae). Hanseniaspora uvarum (Niehaus) (Ascomycota: Saccharomyceta) a yeast closely D. furthermore attractive flies. Based this relation, H. might represent suitable substrate development that more specific than wine. In field, we therefore, compared bait was respect number trapped relative other drosophilids arthropods. Trap captures were higher but specificity greater uvarum. Moreover, headspace extracts, well synthetic blend volatiles, assayed attraction D in wind tunnel field. Headspace extracts induced strong upwind flight confirmed volatiles. Furthermore, baited extract drowning solution aqueous acetic acid ethanol, 74% field captured arthropods suzukii. Our findings suggest formulations advance selective monitoring traps reduced by-catch.

Language: Английский

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Heterologous expression and functional characterization of Drosophila suzukii OR69a transcript variants unveiled response to kairomones and to a candidate pheromone

Journal of Pest Science, Journal Year: 2022, Volume and Issue: 96(3), P. 1149 - 1171

Published: Dec. 14, 2022

Abstract Drosophila suzukii is a main pest of berries on all continents. Population control relies insecticides and current research challenge to develop alternative strategies. Methods based behavior-modifying semiochemicals are widely used against other horticultural insects, the functional characterization chemoreceptors facilitates identification active compounds. Following heterologous expression single olfactory receptors (ORs) in ab3A sensory neurons D. melanogaster , we screened three transcript variants from OR69a-locus (DsuzOR69aA, DsuzOR69aB, DsuzOR69aC), demonstrating binding two possible kairomones (3-octanol R-carvone) fly-emitted volatile compound [(Z)-4-nonenal], although with different pharmacological qualities. By coupling Gas Chromatography SSR (GC-SSR), these ligands enhanced ab3A-spiking at nanogram-aliquots complementary fashion among OR69a-variants, identified another kairomone, methyl salicylate, as most specific ligand for sole DsuzOR69aB. In testing headspaces collected females fly-associated yeast Hanseniaspora uvarum did not observe activation female but headspaces. situ hybridization analysis antennae suggests unique OR69a-subunits neurons, points toward co-expression within same neurons. The constitute cation channels, which kairomone specificity, even if effects coexist (Z)-4-nonenal. Methyl salicylate inspiring future investigation validate potentials this

Language: Английский

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Laboratory evaluation of insect growth regulators against the spotted wing drosophila, Drosophila suzukii

Journal of Pest Science, Journal Year: 2023, Volume and Issue: 97(2), P. 885 - 895

Published: June 21, 2023

Abstract Drosophila suzukii (Diptera: Drosophilidae) is an invasive species that causes great damages to many stone and small fruits, resulting in reduced fruit production high economic losses. The concern about this pest has led the research of a wide range possible control methods. However, main strategies are still based insecticide applications. efficacy different insect growth regulators (IGRs), which have better ecotoxicological profile than conventional pesticides, was evaluated test their feasibility for pest. When adult flies fed three days on artificial diet surface-treated with lufenuron, cyromazine or pyriproxyfen, no offspring developed. progressively recovered when were transferred non-treated diet. continuously 19 diet, pyriproxyfen completely suppressed viable while azadirachtin tebufenozide by more half compared untreated control. Nevertheless, these transient non-permanent effects since produced shortly after females treated results observed additional choice egg-transfer assays indicate vertical transmission lufenuron from whereas effect seems be related feeding larvae although reproductive processes cannot discarded. In addition, showed repellent oviposition pyriproxyfen.

Language: Английский

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