Electroantennogram and machine learning reveal a volatile blend mediating avoidance behavior by Tuta absoluta females to a wild tomato plant

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 27, 2022

Abstract Tomato cultivation is threatened by the infestation of nocturnal invasive tomato pinworm, Tuta absoluta . This study was based on field observations that a wild plant, Solanum lycopersicum var. cerasiforme , grown in Mount Kenya region, Kenya, less attacked T. absoluta, unlike cultivated plants like S. (var. Rambo F1). We hypothesized plant may be actively avoided gravid females because emission repellent allelochemical constituents. Therefore, we compared levels pest monocrops and intercrops two genotypes, characterized headspace volatiles, then determined compounds detectable insect through gas chromatography-linked electroantennography (GC-EAG), finally performed bioassays using blend four EAG-active unique to tomato. found significant reductions monocrop tomato, plant. Quantitative qualitative differences were noted between volatiles plants, day night volatile collections. The most discriminating treatments varied with variable selection or machine learning methods used. In GC-EAG recordings, 16 including hexanal, ( Z )-3-hexenol, α -pinene, β -myrcene, -phellandrene, E )- -ocimene, terpinolene, limonene oxide, camphor, citronellal, methyl salicylate, -caryophyllene, others tentatively identified as 3,7,7-Trimethyl-1,3,5-cycloheptatriene, germacrene D cis -carvenone oxide detected antennae females. Among these compounds, -caryophyllene -phellandrene are top 5 highlighted methods. A citronellal only showed dose-dependent repellence wind tunnel. provides some groundwork for exploiting allelochemicals development novel integrated management approaches against

Language: Английский

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Expression and sex pheromone-binding characteristics of pheromone-binding protein 3 in Tuta absoluta (Lepidoptera: Gelechiidae)

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106404 - 106404

Published: April 1, 2025

Language: Английский

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Tritrophic Interactions Mediated by Zoophytophagous Predator-Induced Host Plant Volatiles

Journal of Chemical Ecology, Journal Year: 2024, Volume and Issue: 50(11), P. 663 - 678

Published: May 9, 2024

Abstract The zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including invasive leafminer, Phthorimaea absoluta , a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. can influence tritrophic interactions plant. Here, we tested hypothesis that phytophagy would olfactory between host plant pest, predator, parasitoid. In olfactometer assays, P. females adults were both attracted to constitutive volatiles released Whereas avoided -infested plants, S. induced volatiles. coupled gas chromatography-electroantennographic detection (GC-EAD) recordings intact volatiles, antennae detected eight components, whereas seven components which identified GC-mass spectrometry (GC-MS) as terpenes green leaf (GLVs). Dose-response bioassays revealed responses varied with composition concentration blends individual compounds from N -induced Females showed no preference an eight-component blend formulated repellents hexanal, ( Z )-3-hexenyl butanoate, δ-elemene On other hand, attractants E )-2-hexenal, )-3-hexenol, methyl salicylate, β-phellandrene, )-caryophyllene. Likewise, seven-component δ-elemene, )-caryophyllene Our findings suggest there potential use GLVs manage insects interaction.

Language: Английский

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Machine Learning Predicts Non-Preferred and Preferred Vertebrate Hosts of Tsetse Flies (Glossina spp.) Based on Skin Volatile Emission Profiles

Journal of Chemical Ecology, Journal Year: 2025, Volume and Issue: 51(2)

Published: March 7, 2025

Tsetse fly vectors of African trypanosomosis preferentially feed on certain vertebrates largely determined by olfactory cues they emit. Previously, we established that three skin-derived ketones including 6-methyl-5-hepten-2-one, acetophenone and geranyl acetone accounted for avoidance zebra tsetse flies. Here, tested the hypothesis these serve as biomarkers flies to distinguish between non-preferred- preferred-vertebrate hosts. We used coupled gas chromatography/mass spectrometry analyze compare skin volatile emissions two (waterbuck zebra) four preferred- (buffalo, donkey, horse, warthog) vertebrate hosts in wildlife parks Kenya. detected a total 96 organic compounds (VOCs) composed mainly aldehydes, ketones, alcohols, phenols alkanes, which varied with host. Using random forest analysis, found weak correlation skin-odor repellent non-preferred preferred However, plus background odors may be more sensitive chemical signals discriminate These results contribute understanding host preferences their natural habitat across geographic scales.

Language: Английский

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Insight into the host-specificity of a native and a newly introduced parasitoid of Tuta absoluta and prospect for biological control

Biological Control, Journal Year: 2024, Volume and Issue: 191, P. 105464 - 105464

Published: Feb. 16, 2024

New strategies are urgently needed to control Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), one of the most destructive pests tomato crops. Originating from South America, it is spreading rapidly and has evolved resistance common insecticides making highly challenging control. Regarding biological control, parasitoid Necremnus tutae Ribes & Bernardo (Hymenoptera: Eulophidae) widely present around Mediterranean basin adapted invader playing a substantial role in its In addition, neotropical Dolichogenidea gelechiidivoris Marsh Braconidae) established accidentally following host Spain Algeria classical program using this species ongoing Sub-Saharan Africa. Understanding trophic connections between D. gelechiidivoris, N. tutae, targeted T. potential non-target hosts essential assess their value as augmentative or agents promote establishment conservation biocontrol. We conducted host-specificity tests laboratory four European leafminer evaluate specificity these two parasitoids absoluta. then assessed affinity for by exposing sentinel plants with alternative Phthorimaea operculella (Zeller) Gelechiidae) inside greenhouses measuring each species' field parasitism rate. Our results show that tested laboratory, attacked three, while only P. operculella. greenhouse, did not prefer absoluta, whereas preferred latter. The mean rate over three months monitoring period reached 27 % 35 gelechiidivoris. discuss importance understanding effects natural enemy can have

Language: Английский

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Challenges of climate change and air pollution for volatile-mediated plant-parasitoid signalling

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: unknown, P. 101290 - 101290

Published: Oct. 1, 2024

Language: Английский

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Recruitment of Hippodamia variegata by active volatiles from Glycyrrhiza uralensis and Alhagi sparsifolia plants infested with Aphis atrata

Pest Management Science, Journal Year: 2023, Volume and Issue: 80(2), P. 355 - 365

Published: Sept. 11, 2023

Abstract BACKGROUND Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae), a dominant predatory natural enemy species in cotton‐planting, is key biological control agent for aphids China. Our previous study showed that herbivore‐induced plant volatiles (HIPVs) from Glycyrrhiza uralensis (Fisch.) (Fabales: Fabaceae) and Alhagi sparsifolia (Desv.) plants infested with Aphis atrata (Zhang) (Homoptera: Aphididae), were important semiochemicals to locate aphids. However, little was known about the varieties function of active HIPVs two species. RESULTS In this study, results gas chromatography‐electroantennography detection (GC‐EAD) demonstrated seven (butyl acrylate, α‐pinene, butyl isobutyrate, β‐pinene, butyrate, 1,3‐diethylbenzene 1,4‐diethylbenzene) identified damaged elicited antennal responses . Also, chromatograph–mass spectrometry (GC–MS) analysis concentrations significantly higher than those corresponding healthy plants. exhibited varying degrees response each volatile electroantennography (EAG) trials, however, only β‐pinene butyrate attracted behavioral trials conducted laboratory. They also had better trapping effect on cotton fields. CONCLUSION Four compounds (α‐pinene, butyrate) considered most effective attract These findings provide possibilities development attractants. theoretical basis prevention using © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

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Better Together: Volatile-Mediated Intraguild Effects on the Preference of Tuta absoluta and Trialeurodes vaporariorum for Tomato Plants

Journal of Chemical Ecology, Journal Year: 2023, Volume and Issue: 49(11-12), P. 725 - 741

Published: Nov. 4, 2023

Language: Английский

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Disentangling thermal effects using life cycle simulation modelling on the biology and demographic parameters of Dolichogenidea gelechiidivoris, a parasitoid of Tuta absoluta

Journal of Thermal Biology, Journal Year: 2022, Volume and Issue: 107, P. 103260 - 103260

Published: May 21, 2022

Language: Английский

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Interactions between the Entomopathogenic Fungus Metarhizium anisopliae ICIPE 20 and the Endoparasitoid Dolichogenidea gelechiidivoris, and Implications for Combined Biocontrol of Tuta absoluta

Biology, Journal Year: 2022, Volume and Issue: 11(9), P. 1323 - 1323

Published: Sept. 6, 2022

The Integrated Pest Management (IPM) approach have been widely promoted and used for the management of native invasive pests, while use various components IPM can a synergetic, additive, or antagonistic effect on each other; this study evaluated susceptibility

Language: Английский

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