The efficacy of 1-allyloxy-4-propoxybenzene (3c{3,6}) against Varroa destructor mites in honey bee colonies from Maryland, USA

Journal of Insect Science, Journal Year: 2024, Volume and Issue: 24(3)

Published: May 1, 2024

Abstract Varroa destructor Oud (Acari: Varroidae) is a harmful ectoparasite of Apis mellifera L. honey bees causing widespread colony losses in Europe and North America. To control populations these mites, beekeepers have an arsenal different treatments, including both chemical nonchemical options. However, treatments can be labor intensive, has gained resistance to some conventional pesticides, the use other restricted temporally (e.g., cannot applied during periods production). Thus, require additional treatment options for controlling mite populations. The compound 1-allyloxy-4-propoxybenzene (3c{3,6}) diether previously shown strong feeding deterrent against Lepidopteran larvae repellent mosquitoes showed promise as novel acaricide from laboratory early field trials. Here we test effect compound, at 8 g/brood box on wooden release devices, bee colonies located Maryland, USA, using thymol-based commercial product positive control. 3c{3,6} had minimal colonies, but more tests are needed determine whether it affected egg production by queens. Against Varroa3c{3,6} estimated efficacy 78.5%, while thymol 91.3%. still development stage, dose or application method needs revisited.

Language: Английский

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Comparing the efficacy of synthetic Varroacides and Varroa destructor phenotypic resistance using Apiarium and Mason jar bioassay techniques

Pest Management Science, Journal Year: 2023, Volume and Issue: 80(3), P. 1577 - 1592

Published: Nov. 17, 2023

Abstract BACKGROUND Varroa mite, destructor , is a major threat for honey bee, Apis mellifera colonies. Beekeepers have used synthetic Varroacides against mite decades, but resistance to organophosphates, pyrethroids and formamidine has been reported in many locations worldwide. The goals of this study were develop reliable bioassay assess efficacy phenotypic commercial Varroacides. In study, was evaluated using the Apiarium technique comparison Mason jar method. RESULTS Among tested Varroacides, high (89%) Apivar identified when compared Bayvarol (58%), Apistan (44%) CheckMite (6%), 24 h assessment. We also found that toxic bees addition, revealed case Bayvarol, population evaluated. CONCLUSION A laboratory protocol developed method evaluate efficacy. Collectively, findings indicated methodology provided measure Varroacide determine presence V . © 2023 Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf Society Chemical Industry.

Language: Английский

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Natural enemies of Varroa destructor identified from Eastern North American honey bee colonies: a biological survey of candidates for mite control from Maryland, USA

Biocontrol Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Nov. 27, 2024

A survey was conducted of microbes and arthropods associated with Varroa destructor (Acari: Varroidae) mites (hereafter, Varroa) collected from honey bee colonies in Maryland, USA. Live were challenged samples both assessed for their potential as biocontrol agents against the mites. To our knowledge, this is first natural enemies North American colonies. The revealed 21 fungi, 25 bacteria, one nematode, 8 arthropod species colonies, challenge assays using 11 these, including 4 2 a parasitic insects (Hemiptera Hymenoptera), arachnids (Pseudoscorpiones) capable killing Varroa. This assemblage similar to taxa identified surveys elsewhere globally, suggesting there core organisms that are best suited tolerate sometimes-hostile abiotic biotic conditions hive environment. Generally, entomopathogenic fungi bacteria study showed highest efficacy Varroa, some, comparable determined by other researchers, appear be most promising candidates development into commercially viable varroicides. novel nematode killed greater than 90% infected hosts. In addition, behaviours some toward observed indicative predation on

Language: Английский

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Lethal and repellent effect of amitraz, eugenol and thymol against Triatoma infestans, the main vector of Trypanosoma cruzi in the southern of America

Medical and Veterinary Entomology, Journal Year: 2023, Volume and Issue: 37(3), P. 574 - 580

Published: April 13, 2023

The lethal and repellent effect of the synthetic insecticide amitraz botanical insecticides eugenol thymol separately together in binary mixtures was tested against late-stage nymphs a susceptible strain Triatoma infestans, main vector Trypanosoma cruzi, etiological agent Chagas disease, Southern Cone America. For lethality study, LD50 determined for each alone mixture by topical application. combination index (CI) established to quantify interactions occurring between insecticides. using area preference technique. 11 34 times more potent than that eugenol, respectively. Only at high concentrations showed synergistic (CI: 0.3). activity monoterpenes after 30 min exposure significant 780 78 μg/cm2 thymol, residual lasted one week 1170 1560 , whereas managed retain its two weeks 3900 .

Language: Английский

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<b>Influence of amitraz-based products characteristic to control varroa mite population</b>

Published: Dec. 19, 2023

The presence of the mite, Varroa destructor, requires use acaricide treatments in most honeybee colonies. Amitraz is one common substances used by beekeepers. Some mite populations have developed resistance to amitraz leading a loss efficacy amitraz-based treatments. Two products, Apivar and Supatraz were applied same apiary. Both are but different galenics. Thanks field data, dynamic model was simulate action on population. We consider two parameters compare products: daily mortality rate treatment duration. In field, percentage between products not significantly kills faster decreases 90% infestation 28.4 days compared 50.9 for Apivar. modelling, we showed impact has higher during first weeks than lower stabilize varroa population due its release active ingredient Apivar, needing shorter period achieve result. Depending model, conserve good low resistant mites high mites. These results show that comparison with characteristics (duration rate) should be analyzed caution.

Language: Английский

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Do Varroa destructor (Acari: Varroidae) mite flows between Apis mellifera (Hymenoptera: Apidae) colonies bias colony infestation evaluation for resistance selection?

Journal of Insect Science, Journal Year: 2024, Volume and Issue: 24(4)

Published: July 1, 2024

Since the global invasion of ectoparasitic mite Varroa destructor (Anderson and Trueman), selection mite-resistant honey bee (Apis mellifera L.) colonies appears challenging has to date not broadly reduced colony mortality. The low published estimated heritability values for infestation levels could explain limited genetic progresses obtained so far. We hypothesize that intercolonial horizontal transmission differentially affect single located in a given apiary therefore invisibly bias phenotypes. This may be lower regions with density, providing suitable conditions set up evaluation apiaries. To verify these hypotheses, we monitored reinvasion experimental colonies, as well neighboring belonging beekeepers three areas variable densities canton Bern, Switzerland during consecutive beekeeping seasons. Mite immigration fluctuated between apiaries years significantly contributed level. Depending on year, 17-48% mites present at time summer oxalic acid final treatment potentially derived from had occurred since mid-spring. was linked local density or beekeepers' within 2 km. Our results do prove should necessarily established density. However, they highlight high impact management practices levels.

Language: Английский

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Characterisation of proteomic alterations in worker bees in response to amitraz treatment during summer to winter transition in Apis mellifera colonies

Journal of Apicultural Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Nov. 6, 2024

The application of treatments to reduce/control the number Varroa destructor mites within Apis mellifera colonies is a common apicultural practice. effectiveness in reducing from has been extensively studied, however, effects these on bees are poorly characterised. This work utilised label-free quantitative proteomic analysis investigate how presence anti-Varroa treatment amitraz, affected worker bees. Samples A. were isolated one week before amitraz (T0) and 1, 3, 6 8 weeks post-treatment application. trial was conducted during transition period short-lived summer workers long-lived winter workers. results highlight two large shifts; decrease abundance cuticular proteins an increase ribosomal protein Weeks 6, 8. changes Week may not be response exposure alone but also attributed potential change population provides insight into effect honey bee proteome colony it contribute inducing stress individual

Language: Английский

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Evaluation of acaricide toxicity to the parasitic mite (Varroa destructor), honeybee workers (Apis mellifera L.) and their residues in honey and beeswax

International Journal of Sustainable Development and Science, Journal Year: 2022, Volume and Issue: 5(2), P. 39 - 50

Published: June 1, 2022

Evaluate the toxic effects of five acaricides on parasitic mite (Varroa destructor) and honeybee workers (Apis mellifera L.): Denfer 15% (Spirodiclofen), Kofex 24% (Spiromesifen), Metake 20% (Amitraz), Koloum super SC (chlorfenapyr), Abalon 1.8% (Abamactine).These conventional are cheap easy to apply, but prolonged use causes Varroa mites rapidly develop resistance, bee products can become contaminated with residues acaricides.The bioassay test was used detect effects.Spirodiclofen Spiromesifen were most compounds worker LC50 values 0.923 1.195 ug/ml, respectively, after 5 days, Amitraz, Chlorfenapyr, Abamactine least honeybees 1.752, 42.72, 56.868 days.Amitraz, Spirodiclofen V. destructor 0.497, 0.888 2.014 4 h, chlorfenapyr 70.972 76.62 3 h.Varroa infestation rates decreased in brood (75.5% vs 67%) adult (77.16% 100%) colonies treated when compared only non-treated colonies.Detecting residuals by HPLC honey beeswax revealed that they Amitraz residues, did not exceed MRLs established EPA European Commission guidelines.

Language: Английский

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EFICÁCIA DO TRATAMENTO COMBINADO DE AMITRAZ E FLUMETRINA NO CONTROLO DA VARROOSE

Editora Artemis eBooks, Journal Year: 2022, Volume and Issue: unknown, P. 250 - 262

Published: Aug. 26, 2022

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DETERMINATION OF RESIDUES OF AMITRAZ AND FLUVALINATE IN HONEY SAMPLES COLLECTED FROM ÇUKUROVA DISTRICT

Izmir Democracy University Health Sciences Journal, Journal Year: 2022, Volume and Issue: 6(1), P. 1 - 9

Published: Dec. 29, 2022

In this study, it was aimed to investigate whether amitraz and fluvalinate residues were present in the honey samples Çukurova district they would pose a risk for human health. Total 135 used with 15 extracted flower from each of Adana center, Kadirli, Kozan, Osmaniye, Ceyhan, Karataş, Yumurtalık, Tarsus Mersin centre. The represented 1550 beehives centre, 980 1050 650 750 860 740 800 950 Tarsus. that taken 8330. Honey analyzed according Hammerling method using gas chromatograhy capillary column. result analyses, although not found samples, ranging 1.34 33.48 ppm 25 samples. honeys contaminated at which levels public It has been concluded stage production consuming honeys, precautions are required be against pesticide up consumers.

Language: Английский

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