First evidence of the effectiveness of a field application of RNAi technology in reducing infestation of the mite Varroa destructor in the western honey bee (Apis mellifera)

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 27, 2025

Language: Английский

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Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(2)

Published: May 27, 2023

Abstract Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift their hosts, resulting in novel outbreaks that damage forests ecology. Traditional management schemes are unable control such outbreaks, therefore unconventional competitive governance is needed manage forest RNA interference (RNAi) mediated double-stranded (dsRNA) treatment method can be implemented protect trees. Exogenous dsRNA triggers RNAi-mediated gene silencing of a vital gene, suspends protein production, death targeted pathogens pests. successful for many crop insects fungi, however, studies against depleting. Pesticides fungicides based on could used combat caused different parts world. Although has proved its potential, crucial dilemma risks including species-specific selection, delivery methods cannot overlooked. Here, we summarized major fungi insect have genomic information, fungi-and pesticides. Current challenges opportunities target decision, using nanoparticles, direct applications, new mycorrhiza tree protection discussed. importance affordable next-generation sequencing minimize impact non-target species We suggest collaborative research among genomics pathology institutes develop necessary strategies species.

Language: Английский

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Nasonia vitripennis as a parasitoid wasp model for pest-specific dsRNA safety assessment

Entomologia Generalis, Journal Year: 2023, Volume and Issue: 43(1), P. 139 - 146

Published: Feb. 9, 2023

RNA interference (RNAi) is a promising mode of action for pest control.It can play an important role in integrated management (IPM), where it could be used alongside beneficial insects (such as predators and parasitoids) control.However, despite the possibility to design active molecule double stranded (dsRNA) pest-specific, potential adverse effects cannot ruled out priori, thus prompting need safety assessment studies.In this study, jewel wasp Nasonia vitripennis presented parasitoid model evaluation possible unintended that arise from oral exposure exogenously delivered pest-specific dsRNA.First, susceptibility N. ingested dsRNA was tested through worst-case scenario, they were exposed high concentration (1 µg/µl) vitripennis-specific (dsNv_alphaCOP).Ingestion dsNv_alphaCOP resulted 97% wasps mortality by day 16 post treatment when compared control.Once risk established, orally pollen beetle (Brassicogethes aeneus)-specific (dsBa_alphaCOP), after which both lethal sub (development time, fecundity sex ratio) evaluated.In scenario setup, dsBa_alphaCOP did not cause any vitripennis.This study confirms under laboratory conditions RNAi-based control compatible with biological control, based on wasps.Furthermore, contribute guiding development future products.

Language: Английский

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In Silico Analysis of Potential Off-Target Effects of a Next-Generation dsRNA Acaricide for Varroa Mites (Varroa destructor) and Lack of Effect on a Bee-Associated Arthropod

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 317 - 317

Published: March 19, 2025

Double-stranded RNA (dsRNA) biopesticides offer the potential for highly targeted pest control with minimal off-target impacts. Varroa mites (Varroa destructor) are an important of honey bees (Apis mellifera) that primarily managed by synthetic pesticides. A next-generation treatment using a varroa-active dsRNA, vadescana, has been developed to target calmodulin expression in varroa. We evaluated exposure non-target species vadescana. First, we assessed gene silencing effects on 39 arthropods known genomes via bioinformatics. Three mite species, monarch butterflies (Danaus plexippus), fruit flies (Drosophila melanogaster), and European earwigs (Forficula auricularia) showed theoretical effects. These silico results could be used help inform risk assessments. Second, conducted vadescana feeding trials greater wax moth (Galleria mellonella), common beehive associate. There were no significant differences reproduction, survival, or adult F2 wing length between vadescana-fed groups. Male body weight was slightly but significantly lower moths exposed highest dose, such effect observed female moths. Calmodulin unaffected Our hazard assessment vadescana’s lethal sublethal indicates impact following continuous dietary far than any might expected field, line bioinformatics findings. This biopesticide appears varroa-specific likely fewer many current varroa methods.

Language: Английский

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RNAi applications towards environmentally sustainable food security

Current Opinion in Environmental Science & Health, Journal Year: 2025, Volume and Issue: unknown, P. 100612 - 100612

Published: March 1, 2025

Language: Английский

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RNAi Technology Against Forest Pests: Opportunities, Challenges and Future Prospects

Published: Jan. 1, 2025

Language: Английский

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RNA Insecticides and Biodiversity Conservation

Published: Jan. 1, 2025

Language: Английский

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Uniting RNAi Technology and Conservation Biocontrol to Promote Global Food Security and Agrobiodiversity

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: April 25, 2022

Habitat loss and fragmentation, the effects of pesticides, contribute to biodiversity losses unsustainable food production. Given United Nation's (UN's) declaration this decade as UN Decade on Ecosystem Restoration, we advocate combining conservation biocontrol-enhancing practices with use RNA interference (RNAi) pesticide technology, latter demonstrating remarkable target-specificity via double-stranded (ds)RNA's sequence-specific mode action. This specificity makes dsRNA a biosafe candidate for integration into global initiative. Our interdisciplinary perspective conforms UN's declaration, is facilitated by Earth BioGenome Project, an effort valuable RNAi development given its utility in providing whole-genome sequences, allowing identification genetic targets crop pests, potentially relevant sequences non-target organisms. Interdisciplinary studies bringing together techniques are needed, should be examined various crop‒pest systems address problem.

Language: Английский

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Oligonucleotide insecticides and RNA-based insecticides: 16 years of experience in contact using of the next generation pest control agents

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Language: Английский

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Topical delivery of dsRNA in two hemipteran species: Evaluation of RNAi specificity and non-target effects

Pesticide Biochemistry and Physiology, Journal Year: 2022, Volume and Issue: 189, P. 105295 - 105295

Published: Nov. 19, 2022

Language: Английский

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