Optimizing toxic bait composition and application to control Drosophila suzukii DOI Creative Commons

Karla Escobedo Quevedo

Published: June 11, 2024

Invasive pest species are that not endemic to the ecosystem where they found, have established in this new environment, and (are likely to) cause environmental or economic damage these environments harm human health. The most commonly known deleterious effects an invasive can extinction of species, alterations losses by damaging important food crop. be difficult control due lack suitable natural enemies, i.e. invaded area is prepared for them. Currently, there a non-native has almost all continents: scientifically as Drosophila suzukii spotted wing drosophila (SWD). fruit fly causes significant growing industry, stone fruits soft . presents seasonal phenotypic plasticity displaying traits best suit conditions spring/summer autumn/winter. Adults emerge with different morphology physiology response temperature experience during larval and/or pupal development. This thesis focused on development effective sustainable bait spray strategy D. adult females. Bait sprays consist mixture insecticide lure, aiming insects ingest interact thereby facilitating uptake insecticide. I investigated i) Foraging behavior suzukii; ii) Food lure composition; iii) Biorational insecticides toxin; iv) dsRNA toxin. In investigations, included both summer winter morphs, assess whether respond similarly experimental treatments.I found understanding foraging plays role application sprays. My laboratory results showed position placed influenced chances it encountered flies, more feeding towards upper part plant. observed flies approached walking up stalk then exploring leaf surface. When once had stimulus, spent time it, even non-attractive bait. Based this, concluded should necessarily long-distance attractive used sprays, long applied pests expected come into contact it. Upon further study composition its makeup, primarily nutritional value, impacted interaction lures. Flies did show strong preference sugar- yeast-based when were offered at same time. Overall, differences preferences, resistance starvation mobility between morph flies.Then, toxin component comparing biorational insecticides. formulated from plants oil extracts, microorganisms, minerals products relatively innocuous non-target organisms. experiments Mexico Netherlands. could directly compare results, because manufacturers recommended doses also different. However, experiments, similar, tested sufficiently toxic suzukii. Hence, another approach needed. explored possibility using RNAi-based mixed technology based silencing vital gene via administration double-stranded RNA (dsRNA) insect. Gene transcript knockdown mortality target species. considered biodegradable (i.e. do accumulate environment). Moreover, designed highly specific thus would fit very well Integrated Pest Management strategies. neither nor mortality. Because potentially great benefits, recommend continuing research technique elucidate mechanisms delivering cells.This provided knowledge females behavior. characterization forementioned direct implications optimizing Additionally, believe potential combined It mention done under need extrapolated agriculture settings. therefore field conditions.

Language: Английский

RNA Interference in Insects: From a Natural Mechanism of Gene Expression Regulation to a Biotechnological Crop Protection Promise DOI Creative Commons
Beltrán Ortolá, José‐Antonio Daròs

Biology, Journal Year: 2024, Volume and Issue: 13(3), P. 137 - 137

Published: Feb. 21, 2024

Insect pests rank among the major limiting factors in agricultural production worldwide. In addition to direct effect on crops, some phytophagous insects are efficient vectors for plant disease transmission. Large amounts of conventional insecticides required secure food worldwide, with a high impact economy and environment, particularly when beneficial also affected by chemicals that frequently lack desired specificity. RNA interference (RNAi) is natural mechanism gene expression regulation protection against exogenous endogenous genetic elements present most eukaryotes, including insects. Molecules double-stranded (dsRNA) or highly structured substrates cellular enzymes produce several types small RNAs (sRNAs), which play crucial role targeting sequences transcriptional post-transcriptional silencing. The relatively simple rules underlie RNAi regulation, mainly based Watson–Crick complementarity, have facilitated biotechnological applications these mechanisms. This includes promise using engineered dsRNA molecules, either endogenously produced crop plants exogenously synthesized applied onto as new generation specific, sustainable, environmentally friendly insecticides. Fueled this expectation, article reviews current knowledge about pathways insects, other questions such delivery recombinant RNA, critical establish reliable technology insect control plants.

Language: Английский

Citations

17
SIGS vs. HIGS: opportunities and challenges of RNAi pest and pathogen control strategies DOI Creative Commons

Bliss M. Beernink,

Nazanin Amanat,

Vivian H. Li

et al.

Canadian Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Sept. 4, 2024

Language: Английский

Citations

8
pH-responsive nanoparticles for oral delivery of RNAi for sustained protection against Spodoptera exigua DOI

Yiping Jiang,

Suman Zong,

Xiaofeng Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141763 - 141763

Published: March 4, 2025

Language: Английский

Citations

0
Engineered nanotransporters for efficient RNAi delivery in plant protection applications DOI Open Access
Yue Xing,

Hao Jiang,

Lin Cai

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

ABSTRACT RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes tissue damage, affected by environmental factors, faces difficulties in penetrating barriers of cell walls limitations species, ultimately leading to low efficiency. With advances nanotechnology, nanomaterials (NMs) have been identified as effective carriers nucleic acid because their ability operate independently external mechanical forces, prevent degradation bioenzymes, exhibit good biocompatibility, offer high loading capacity. This review summarizes application NM‐mediated RNAi pests, focusing on how different NMs break through plants, pathogens, pests according size, morphology, charge characteristics. Furthermore, we discuss advantages improvement strategies carriers, alongside assessing potential management

Language: Английский

Citations

0
Silence is not always golden: A closer look at potential environmental and ecotoxicological impacts of large-scale dsRNA application DOI

Mohammad Zarrabian,

Sherif M Sherif

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175311 - 175311

Published: Aug. 8, 2024

Language: Английский

Citations

3
Functionally Modified Graphene Oxide as an Alternative Nanovehicle for Enhanced dsRNA Delivery in Improving RNAi-Based Insect Pest Control DOI
Qi Xue,

Jiangjie Li,

Sven Vereecken

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

RNA interference (RNAi) has shown substantial promise as a sustainable pest management solution. However, the efficacy of RNAi-based insecticides heavily relies on advanced nanocarrier-mediated delivery systems. In this study, we modified raw graphene oxide into positively charged nanocarriers (GONs) tailored to bind with double-stranded (dsRNA). The resulting GONs@dsRNA complexes demonstrated small particle size (106 nm) and maintained stability under various conditions, including insect gut extracts, extreme pH, temperature. Furthermore, GONs efficiently transported dsRNA molecules Drosophila S2 cells Lepidoptera Sf9 cells, leading an enhanced target transcript knockdown. Targeting vacuolar ATPase gene,

Language: Английский

Citations

2
Approved natural products-derived nanomedicines for disease treatment DOI
Xiaotong Li,

Yu-Ying Lai,

Guihong Wan

et al.

Chinese Journal of Natural Medicines, Journal Year: 2024, Volume and Issue: 22(12), P. 1100 - 1116

Published: Dec. 1, 2024

Language: Английский

Citations

1
Drosophila X virus‐like particles as delivery carriers for improved oral insecticidal efficacy of scorpion Androctonus australis peptide against the invasive fruit fly, Drosophila suzukii DOI Open Access
Qi Xue, Luc Swevers, Clauvis Nji Tizi Taning

et al.

Insect Science, Journal Year: 2023, Volume and Issue: 31(3), P. 847 - 858

Published: Sept. 8, 2023

Abstract Insect‐specific neurotoxic peptides derived from the venoms of scorpions and spiders can cause acute paralysis death when injected into insects, offering a promising insecticidal component for insect pest control. However, effective delivery systems are required to help pass through gut barrier hemolymph, where they act. Here, we investigated potential novel nanocarrier, Drosophila X virus‐like particle (DXV‐VLP), delivering neurotoxin scorpion Androctonus australis Hector (AaIT) against invasive fruit fly, suzukii . Our results show that fusion proteins DXV polyproteins with AaIT peptide at their C‐termini could be sufficiently produced in Lepidoptera Hi5 cells soluble form using recombinant baculovirus expression system, self‐assemble VLPs similar morphology size authentic virions. In addition, displayed on DXV‐VLPs retained toxicity, as demonstrated injection bioassays resulted severe mortality (72%) adults after 72 h. When fed adults, mild was observed group treated DXV‐AaIT (38%), while no occurred peptide, thus indicating significant role peptides. Overall, this proof‐of‐concept study demonstrates first time exploited enhance oral insect‐specific context Moreover, it provides insights further improvements potentially development neurotoxin‐based bioinsecticides and/or transgenic crops

Language: Английский

Citations

2
Optimizing toxic bait composition and application to control Drosophila suzukii DOI Creative Commons

Karla Escobedo Quevedo

Published: June 11, 2024

Invasive pest species are that not endemic to the ecosystem where they found, have established in this new environment, and (are likely to) cause environmental or economic damage these environments harm human health. The most commonly known deleterious effects an invasive can extinction of species, alterations losses by damaging important food crop. be difficult control due lack suitable natural enemies, i.e. invaded area is prepared for them. Currently, there a non-native has almost all continents: scientifically as Drosophila suzukii spotted wing drosophila (SWD). fruit fly causes significant growing industry, stone fruits soft . presents seasonal phenotypic plasticity displaying traits best suit conditions spring/summer autumn/winter. Adults emerge with different morphology physiology response temperature experience during larval and/or pupal development. This thesis focused on development effective sustainable bait spray strategy D. adult females. Bait sprays consist mixture insecticide lure, aiming insects ingest interact thereby facilitating uptake insecticide. I investigated i) Foraging behavior suzukii; ii) Food lure composition; iii) Biorational insecticides toxin; iv) dsRNA toxin. In investigations, included both summer winter morphs, assess whether respond similarly experimental treatments.I found understanding foraging plays role application sprays. My laboratory results showed position placed influenced chances it encountered flies, more feeding towards upper part plant. observed flies approached walking up stalk then exploring leaf surface. When once had stimulus, spent time it, even non-attractive bait. Based this, concluded should necessarily long-distance attractive used sprays, long applied pests expected come into contact it. Upon further study composition its makeup, primarily nutritional value, impacted interaction lures. Flies did show strong preference sugar- yeast-based when were offered at same time. Overall, differences preferences, resistance starvation mobility between morph flies.Then, toxin component comparing biorational insecticides. formulated from plants oil extracts, microorganisms, minerals products relatively innocuous non-target organisms. experiments Mexico Netherlands. could directly compare results, because manufacturers recommended doses also different. However, experiments, similar, tested sufficiently toxic suzukii. Hence, another approach needed. explored possibility using RNAi-based mixed technology based silencing vital gene via administration double-stranded RNA (dsRNA) insect. Gene transcript knockdown mortality target species. considered biodegradable (i.e. do accumulate environment). Moreover, designed highly specific thus would fit very well Integrated Pest Management strategies. neither nor mortality. Because potentially great benefits, recommend continuing research technique elucidate mechanisms delivering cells.This provided knowledge females behavior. characterization forementioned direct implications optimizing Additionally, believe potential combined It mention done under need extrapolated agriculture settings. therefore field conditions.

Language: Английский

Citations

0