Chromosome‐level genome assembly and comparative genomics shed light on Helicoverpa assulta ecology and pest management
Pest Management Science,
Journal Year:
2024,
Volume and Issue:
80(10), P. 5440 - 5451
Published: June 28, 2024
Abstract
BACKGROUND
The
Oriental
tobacco
budworm,
Helicoverpa
assulta
,
a
specialist
herbivorous
insect
that
exclusively
feeds
on
plants
of
the
Solanaceae
family,
causes
considerable
damage
to
crops,
such
as
and
hot
pepper.
absence
genome
sequence
for
this
species
hinders
further
research
its
pest
management
ecological
adaptation.
RESULTS
Here,
we
present
high‐quality
chromosome‐level
Korean
strain
H.
(Pyeongchang
strain,
K18).
total
assembly
spans
424.4
Mb
with
an
N50
length
14.54
37%
GC
content.
assembled
(ASM2961881v1)
comprises
31
chromosomes,
similar
other
congeneric
generalist
including
armigera
zea
.
In
terms
genomic
quality,
complete
BUSCOs
repeat
content
accounted
98.3%
33.01%
genome,
respectively.
Based
assembly,
19
485
protein‐coding
genes
were
predicted
in
annotation.
A
comparative
analysis
was
conducted
using
identified
number
(24154)
(23696).
Out
genes,
137
15
orthogroups
found
have
expanded
significantly
while
149
95
contracted
rapidly.
CONCLUSION
This
study
revealed
specific
gene
expansions
contractions
compared
those
close
relatives,
indicating
potential
adaptations
related
specialized
feeding
habits.
Also,
provides
valuable
insights
integrated
globally
significant
pests
Heliothinae
subfamily.
©
2024
Society
Chemical
Industry.
Language: Английский
Plasticity in Gene Expression Patterns and CYPSF Gene Possibly Involved in the Etofenprox-Resistant Population of White-Backed Planthopper, Sogatella furcifera
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13605 - 13605
Published: Dec. 19, 2024
The
white-backed
planthopper
(WBPH)
poses
a
significant
threat
to
rice
crops
globally.
A
bioassay
was
conducted
on
three
WBPH
populations
collected
from
Korean
fields
assess
the
effectiveness
of
five
insecticides,
including
etofenprox
and
fenobucarb.
results
showed
mortality
rate
over
97%
at
recommended
concentration
for
carbamate
organophosphate
insecticides.
However,
exhibited
less
than
40%
in
all
tested
with
Jindo
population
showing
highest
resistance.
No
mutations
were
identified
voltage-sensitive
sodium
channel,
target
site
etofenprox,
suggesting
an
alternative
resistance
mechanism.
To
explore
this,
RNA-seq
analysis
performed
identify
genes
potentially
associated
Gene
expression
assessed
after
treatment
two
sublethal
doses
using
population.
revealed
that
CYPSF
gene,
part
CYP6
family,
consistently
overexpressed
both
treated
untreated
samples.
This
observation
aligns
results,
where
increased
significantly
cytochrome
P450
inhibitor
PBO,
indicating
may
play
key
role
Additionally,
distinct
gene
patterns
different
concentrations
suggest
metabolic
mechanisms
be
involved.
Language: Английский