Progress in the study of association between hematological indicators and retinopathy of prematurity (Review) DOI Open Access
Wenwen Tang, Yin Zhang, Haifang Zhang

et al.

Biomedical Reports, Journal Year: 2024, Volume and Issue: 21(2)

Published: June 6, 2024

Retinopathy of prematurity (ROP) is a retinopathy caused by abnormal proliferation blood vessels in premature infants. It can lead to retinal detachment and, severe cases, blindness, rendering ROP critical condition. Advances neonatal medicine have improved survival rates low birth weight and gestational age However, this progress has also led rise incidence ROP. Currently, birth, high postpartum oxygen levels are independent risk factors for Other include mode delivery, multiple births, anemia, transfusion, maternal pregnancy factors, bronchopulmonary dysplasia, use surfactants, arterial ductus arteriosus necrotizing enterocolitis. Laboratory indicators infants such as platelet count, glucose, inflammatory cells, lipid hemoglobin transfusion may be associated with the etiology pathogenesis not fully understood. A number influence onset progression ROP, including decreased counts, levels, increased white cell glucose disorders metabolism. The present study reviewed effects hemoglobin, cells lipids, plasma metabolic pathways on

Language: Английский

Systemic regulation of retinal medium-chain fatty acid oxidation repletes TCA cycle flux in oxygen-induced retinopathy DOI Creative Commons
Almut Heinken, John M. Asara, Gopalan Gnanaguru

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 9, 2025

Activation of anaplerosis takes away glutamine from the biosynthetic pathways to energy-producing TCA cycle. Especially, induction hyperoxia driven in neurovascular tissues such as retina during early stages development could deplete precursors newly proliferating endothelial cells impeding physiological angiogenesis and leading vasoobliteration. Using an oxygen-induced retinopathy (OIR) mouse model, we investigated metabolic differences between OIR-resistant BALB/cByJ OIR susceptible C57BL/6J strains at system levels understand molecular underpinnings that potentially contribute hyperoxia-induced vascular abnormalities neural retina. Our systems level vivo RNA-seq, proteomics, lipidomic profiling ex-vivo retinal explant studies show medium-chain fatty acids serves alternative source feed findings strongly implicate suppress glutamine-fueled ameliorate conditions prematurity. Medium chain acid metabolism differs resistant strains. Hyperoxia induces medium utilization preventing hyperoxic conditions.

Language: Английский

Citations

0
Differential expression profiling and functional prediction of circRNA in hypoxia-induced human retinal microvascular endothelial cells DOI Creative Commons

Juan Chen,

Ning Wang, Jing Li

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract Background Retinopathy of prematurity (ROP) is a severe retinal disorder primarily affecting premature infants, characterized by abnormal blood vessel development. The pathogenesis ROP closely linked to hypoxic conditions, with human microvascular endothelial cells (HRMECs) being particularly sensitive hypoxia. Despite advancements in understanding ROP, the precise molecular mechanisms underlying its development remain poorly understood. Recent studies have identified involvement circular RNAs (circRNAs) various diseases, including disorders, but their role remains unclear. Methods This study established cell model using HRMECs and analyzed differential expression profiles circRNAs induced hypoxia whole-transcriptome sequencing. Differentially expressed (DE-circRNAs) were validated through qRT-PCR, functional analyses, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis, conducted. Bioinformatics techniques employed construct circRNA-miRNA-mRNA regulatory networks. Results A total 1,714 differentially between normoxic groups, 899 upregulated 815 downregulated (fold-change ≥ 2, p ≤ 0.05). GO analysis revealed that DE-circRNAs involved cellular processes, while KEGG several associated pathways. CircRNA-miRNA-mRNA networks indicated may contribute circRNA-targeted miRNA-mRNA axis. Conclusions profile HRMEC model. findings suggest hsa_circ_0140253/hsa-miR-210-3p/ERFR hsa_circ_0140253/hsa-miR-210-3p/PPARGC1A pathways are via sponge mechanism.

Language: Английский

Citations

0
Age-related driving mechanisms of retinal diseases and neuroprotection by transcription factor EB-targeted therapy DOI Creative Commons
Samuel Abokyi, Dennis Y. Tse

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(2), P. 366 - 377

Published: April 3, 2024

Retinal aging has been recognized as a significant risk factor for various retinal disorders, including diabetic retinopathy, age-related macular degeneration, and glaucoma, following growing understanding of the molecular underpinnings their development. This comprehensive review explores mechanisms investigates potential neuroprotective approaches, focusing on activation transcription EB. Recent meta-analyses have demonstrated promising outcomes EB-targeted strategies, such exercise, calorie restriction, rapamycin, metformin, in patients animal models these common diseases. The critically assesses role EB biology during aging, its effects, therapeutic disorders. impact is cell-specific, influencing metabolic reprogramming energy homeostasis neurons through regulation mitochondrial quality control nutrient-sensing pathways. In vascular endothelial cells, controls important processes, cell proliferation, tube formation, nitric oxide levels, thereby inner blood-retinal barrier, angiogenesis, microvasculature. Additionally, affects smooth muscle inhibiting calcification atherogenesis. pigment epithelial modulates functions autophagy, lysosomal dynamics, clearance lipofuscin, promoting photoreceptor survival regulating growth A expression involved neovascularization. These cell-specific significantly encompassing proteostasis, neuronal synapse plasticity, metabolism, microvasculature, inflammation, ultimately offering protection against emphasizes target Therefore, it imperative to obtain well-controlled direct experimental evidence confirm efficacy modulation diseases while minimizing adverse effects.

Language: Английский

Citations

3
Exploring endocrine FGFs - structures, functions and biomedical applications DOI
Phuc Phan,

Gaёtane Ternier,

Oshadi Edirisinghe

et al.

International Journal of Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 15(4), P. 68 - 99

Published: Jan. 1, 2024

The family of fibroblast growth factors (FGFs) consists 22 members with diverse biological functions in cells, from cellular development to metabolism. can be further categorized into three subgroups based on their modes action. FGF19, FGF21, and FGF23 are endocrine FGFs that act a hormone-like/endocrine manner regulate various metabolic activities. However, all the require both FGF receptors (FGFRs) klotho co-receptors elicit functions. α-klotho β-klotho as scaffolds bring closer form active complexes. Numerous novel studies about FGFs' structures, mechanisms, physiological insights have been published understand complex molecular interactions activities FGFs. Herein, we aim review functions, binding mechanisms cognate receptors, biomedical applications recent years.

Language: Английский

Citations

1
Bidirectional causality of physical exercise in retinal neuroprotection DOI Creative Commons
Stephen Kugbere Agadagba, Suk-Yu Yau, Ying Liang

et al.

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(12), P. 3400 - 3415

Published: Dec. 16, 2024

Physical exercise is recognized as an effective intervention to improve mood, physical performance, and general well-being. It achieves these benefits through cellular molecular mechanisms that promote the release of neuroprotective factors. Interestingly, reduced levels have been implicated in several central nervous system diseases, including ocular disorders. Emerging evidence has suggested are significantly lower individuals with diseases such glaucoma, age-related macular degeneration, retinitis pigmentosa, diabetic retinopathy. may a effect on retina. Therefore, association between involve bidirectional causal relationship whereby visual impairment leads decreased exacerbates development disease. In this review, we summarize linking eye disease identify potential mediators exercise-induced retinal neuroprotection. Finally, discuss future directions for preclinical clinical research health.

Language: Английский

Citations

1
Postnatal hyperglycemia alters amino acid profile in retinas (model of Phase I ROP) DOI Creative Commons
Jarrod C. Harman, Aldina Pivodic, Anders K. Nilsson

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 108021 - 108021

Published: Sept. 22, 2023

Nutritional deprivation occurring in most preterm infants postnatally can induce hyperglycemia, a significant and independent risk factor for suppressing physiological retinal vascularization (Phase I retinopathy of prematurity (ROP)), leading to compensatory but pathological neovascularization. Amino acid supplementation reduces neovascularization mice. Little is known about amino contribution Phase ROP. In mice modeling hyperglycemia-associated ROP, we found changes acids (including decreased L-leucine, L-isoleucine, L-valine). Parenteral L-isoleucine suppressed vascularization. premature infants, severe ROP was associated with higher mean intake parenteral versus enteral the first two weeks life after adjustment treatment group, gestational age at birth, birth weight, sex. The number days support independently predicted Further understanding modulating may help improve nutritional intervention prevent

Language: Английский

Citations

3
Systems levels analysis of lipid metabolism in oxygen-induced retinopathy DOI Open Access
Charandeep Singh

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 22, 2023

Hyperoxia induces glutamine-fueled anaplerosis in the Muller cells, endothelial and retinal explants. Anaplerosis takes away glutamine from biosynthetic pathway to energy-producing TCA cycle. This process depletes precursors newly proliferating cells. The induction of hyperoxic retina is a compensatory response, either decreased glycolysis or flux We hypothesized that by providing substrates feed into TCA, we could reverse prevent anaplerosis, thereby abating wastage for energy generation. Using an oxygen-induced retinopathy (OIR) mouse model, first compared difference fatty acid metabolism between OIR-resistant BALB/cByJ OIR susceptible C57BL/6J strains understand if these exhibit metabolic protects conditions prevents their vasculature model. Based on our findings comparison two strains, medium-chain acid, octanoate, can serve as alternative source response hyperoxia. Our systems levels analysis model shows medium chain TCA. here, time, demonstrate use octanoate replenish normoxic at higher rate conditions.

Language: Английский

Citations

1
Protective Effects of Butyrate on Retinal Neovascularization in Preclinical Retinopathy of Prematurity Models DOI Open Access

Allston Oxenrider,

Tommy Bui,

John Lester

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 4, 2024

Abstract Retinopathy of prematurity (ROP) remains a leading cause childhood blindness worldwide, necessitating new therapeutic strategies. Current interventions targeting advanced disease stages often fail to prevent long-term visual impairment. This study investigates the potential sodium butyrate (NaB), an orally administered short-chain fatty acid, in preclinical models ROP. Using oxygen-induced retinopathy (OIR) mouse model, we demonstrate that daily oral NaB supplementation significantly protects against pathological angiogenesis, impacting not only vascular but also neuronal and microglial pathology inner retina. Notably, shows efficacy early-phase ROP intervention, as evidenced by studies post-natal day 9 (P9) OIR mice novel hyperglycemia-associated (HAR) which mimics hyperglycemic conditions many premature infants. These findings highlight promising alternative or adjunct therapy current anti-VEGF treatments, offering protection across multiple retinal cell types development. The underscores need for further research elucidate specific mechanisms NaB’s action, paving way its clinical application management. marks first exploration preventative agent ROP, setting stage additional evaluations optimization. Highlights Sodium (NaB) significant angiogenesis model. impacts highlights intervention (ROP). is investigate

Language: Английский

Citations

0
Progress in the study of association between hematological indicators and retinopathy of prematurity (Review) DOI Open Access
Wenwen Tang, Yin Zhang, Haifang Zhang

et al.

Biomedical Reports, Journal Year: 2024, Volume and Issue: 21(2)

Published: June 6, 2024

Retinopathy of prematurity (ROP) is a retinopathy caused by abnormal proliferation blood vessels in premature infants. It can lead to retinal detachment and, severe cases, blindness, rendering ROP critical condition. Advances neonatal medicine have improved survival rates low birth weight and gestational age However, this progress has also led rise incidence ROP. Currently, birth, high postpartum oxygen levels are independent risk factors for Other include mode delivery, multiple births, anemia, transfusion, maternal pregnancy factors, bronchopulmonary dysplasia, use surfactants, arterial ductus arteriosus necrotizing enterocolitis. Laboratory indicators infants such as platelet count, glucose, inflammatory cells, lipid hemoglobin transfusion may be associated with the etiology pathogenesis not fully understood. A number influence onset progression ROP, including decreased counts, levels, increased white cell glucose disorders metabolism. The present study reviewed effects hemoglobin, cells lipids, plasma metabolic pathways on

Language: Английский

Citations

0