Substantially altered bacterial diversity associated with developmental stages of litchi stink bug, Tessaratoma javanica (Thunberg) (Hemiptera: Tessaratomidae) DOI Creative Commons
Anita Kumari, Jaipal Singh Choudhary, Anand Kumar Thakur

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e32384 - e32384

Published: June 1, 2024

The mutualistic symbiotic relationship between insects and bacteria greatly influences the growth development of host insects. Tessaratoma javanica (Thunberg) (Hemiptera: Tessaratomidae), also referred to as litchi stink bug, has recently been established an important insect pest Litchi chinensis Sonn. causes substantial yield loss in India. To design effective environmentally safe management strategies, understanding diversity functions microbiota harbored across stages is very important. assessment development-associated T. their predicted was conducted using 16S rRNA gene sequences obtained by Illumina MiSeq technology. result showed that taxonomic analysis associated different developmental includes a total 46 phyla, encompassing 139 classes, 271 orders, 474 families, 893 genera bacteria. All shared 42.82 percent operational units (OTUs), with 97% similarity threshold. Alpha indices maximum species richness egg adult stages. phyla Proteobacteria followed Firmicutes, Bacteriodetes, Actinobacteria, exhibited highest levels abundance all javanica. Microbiota were most 4th nymphal stage (χ2 =711.67) least 2nd instars = 44.45). are mainly involved amino acid metabolism, cell motility, cellular processes signaling, glycan biosynthesis lipid membrane transport. present study documentation information on life will prompt novel biological strategies.

Language: Английский

A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes DOI
Liming Zhang, Daxi Wang, Peibo Shi

et al.

Science, Journal Year: 2024, Volume and Issue: 384(6693)

Published: April 18, 2024

The commensal microbiota of the mosquito gut plays a complex role in determining vector competence for arboviruses. In this study, we identified bacterium from field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered refractory to infection with dengue and Zika viruses. Inoculation 1.6 × 10 3 colony forming units (CFUs) into A. effectively prevents viral infection. Mechanistically, secretes glucose dehydrogenase Ry GDH), which acidifies lumen fed mosquitoes, causing irreversible conformational changes flavivirus envelope protein prevent entry cells. semifield conditions, exhibits effective transstadial transmission blocks virus by newly emerged adult mosquitoes. prevalence is greater low-dengue areas (52.9 ~91.7%) than those dengue-endemic regions (0 ~6.7%). may offer an safe lead biocontrol.

Language: Английский

Citations

23
Substantially altered bacterial diversity associated with developmental stages of litchi stink bug, Tessaratoma javanica (Thunberg) (Hemiptera: Tessaratomidae) DOI Creative Commons
Anita Kumari, Jaipal Singh Choudhary, Anand Kumar Thakur

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e32384 - e32384

Published: June 1, 2024

The mutualistic symbiotic relationship between insects and bacteria greatly influences the growth development of host insects. Tessaratoma javanica (Thunberg) (Hemiptera: Tessaratomidae), also referred to as litchi stink bug, has recently been established an important insect pest Litchi chinensis Sonn. causes substantial yield loss in India. To design effective environmentally safe management strategies, understanding diversity functions microbiota harbored across stages is very important. assessment development-associated T. their predicted was conducted using 16S rRNA gene sequences obtained by Illumina MiSeq technology. result showed that taxonomic analysis associated different developmental includes a total 46 phyla, encompassing 139 classes, 271 orders, 474 families, 893 genera bacteria. All shared 42.82 percent operational units (OTUs), with 97% similarity threshold. Alpha indices maximum species richness egg adult stages. phyla Proteobacteria followed Firmicutes, Bacteriodetes, Actinobacteria, exhibited highest levels abundance all javanica. Microbiota were most 4th nymphal stage (χ2 =711.67) least 2nd instars = 44.45). are mainly involved amino acid metabolism, cell motility, cellular processes signaling, glycan biosynthesis lipid membrane transport. present study documentation information on life will prompt novel biological strategies.

Language: Английский

Citations

0