Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antibacterial activity DOI Creative Commons
Xiaowei Li, Yanhan Chen, Shunyi Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Background Bacillus is utilized as a biological control agent in agricultural production. The main mechanisms accountable for the biocontrol activity encompass generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion growth and induction plant resistance, thereby enhancing inhibition pathogenic fungi. It regarded having high potential has turned into research hotspot. Results We found that strain KC14-1 had significant inhibitory effects on Fusarium Fujikuroi, Rhizoclonia Solani, Alternaria oxysporum Valsa mali. Based morphological observations, physiological biochemical determinations, 16S rRNA, gyrA, gyrB gene sequencing, was identified Bacillus subtilis. Whole sequencing results showed genome composed ring chromosome 3908079 bp size, GC content 43.82%, 3895 coding genes. Anti-SMASH predicted contained nine clusters synthesised antibacterial substances. homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, bacilysin 100%. Conclusion subtilis determined through whole-genome analysis. Our study provides solid foundation development utilisation this strain.

Language: Английский

First report of the agricultural biocontrol agent Bacillus velezensis and foodborne outbreak due to rope spoilage in cakes DOI Creative Commons
Donald E. Brannen,

John Marks,

Richard Schairbaum

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT This paper presents an outbreak involving retail bakery cakes and a celebration at business with multiple sites. Bacillus spore-forming bacteria have been identified as the cause of “ropey bread” spoilage for over 100 years. Causative strains rope include B. subtilis other species. Thirty-five employees having event across 11 sites were questioned to characterize amount cake eaten, observations, symptoms. No human specimens obtained, but leftover was analyzed by Ohio Department Health Bureau Public Laboratory. The odds that caused symptoms 9.23 (1.02–83). presence odor decreased eaten 151 g (103–205 g), P = 0.001. Enteric developed 0.41–4.5 hours after exposure, 5 12 cases latent diarrhea. Cakes positive velezensis . appears be first mention contributor re-emergence in industry. Updates taxonomy starting 1973 may account why are historically listed without spoilage. Given ability effective biocontrol agent grow pH water activity levels like many baked goods, there is need study issues along entire food chain balance impact on additives production safety. IMPORTANCE

Language: Английский

Citations

0
Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity DOI Creative Commons
Xiaowei Li, Yahan Chen, Shunyi Yang

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 31, 2025

Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion growth, and induction plant resistance, enhancing inhibition pathogenic fungi. has high potential become research hotspot. It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria oxysporum, Valsa mali. Based morphological observations, physiological biochemical determinations, 16 S rRNA, gyrA, gyrB gene sequencing, identified subtilis. Whole genome sequencing results showed composed ring chromosome 3,908,079 bp size, GC content 43.82% 3,895 coding genes. Anti-SMASH predicted contained nine clusters synthesised antibacterial substances. homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, bacilysin 100%. subtilis determined through whole-genome analysis. Our study provides solid foundation developing utilising this strain.

Language: Английский

Citations

0
Characterization of Biofertilization and Biocontrol Potential of Bacillus velezensis KHH13 from Organic Soils DOI Creative Commons
Tai-Yuan Chen, Yuh Tzean,

Tsai-De Chang

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(6), P. 1135 - 1135

Published: May 26, 2024

Efficient and sustainable food production is crucial in global agricultural development. Overuse of chemical fertilizers leads to soil acidification, destruction properties, harm micro-organisms. Plant growth-promoting rhizobacteria (PGPR) have emerged as a solution, enhancing fertility crop yields while reducing fertilizer dependency disease occurrence. In this study, Bacillus strains KHC2, KHH5, KHH13, isolated from organic rice field soils Taiwan, were identified through molecular techniques B. velezensis (KHC2, KHH13) amyloliquefaciens (KHH5). The exhibited various hydrolytic enzymes (including protease, cellulase, amylase, lecithinase), with KHH13 showing the highest phosphate solubilization (2186.1 µg mL−1 day−1) indole-3-acetic acid (IAA) (63.067 ± 0.595 ppm mL−1). These properties indicate KHH13’s potential bio-enhancer for plant growth. Therefore, we hypothesized that can enhance growth control soil-borne diseases. A greenhouse experiment demonstrated KHH5 effectively promoted red lettuce, superior efficacy. study also found treatment enhanced vegetables, including tomato, cucumber, lettuce. terms control, significantly reduced Fusarium wilt cucumbers, evidenced by reduction index 74.33% 41.67% after treatment. group displayed better growth, height fresh weight, compared experiment. Furthermore, oral pulmonary acute toxicity analysis rats showed no adverse effects on rat weight or mortality, indicating safety mammalian use. findings suggest safe, effective, biological agent vegetable controlling diseases, applications agriculture.

Language: Английский

Citations

1
Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antibacterial activity DOI Creative Commons
Xiaowei Li, Yanhan Chen, Shunyi Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Background Bacillus is utilized as a biological control agent in agricultural production. The main mechanisms accountable for the biocontrol activity encompass generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion growth and induction plant resistance, thereby enhancing inhibition pathogenic fungi. It regarded having high potential has turned into research hotspot. Results We found that strain KC14-1 had significant inhibitory effects on Fusarium Fujikuroi, Rhizoclonia Solani, Alternaria oxysporum Valsa mali. Based morphological observations, physiological biochemical determinations, 16S rRNA, gyrA, gyrB gene sequencing, was identified Bacillus subtilis. Whole sequencing results showed genome composed ring chromosome 3908079 bp size, GC content 43.82%, 3895 coding genes. Anti-SMASH predicted contained nine clusters synthesised antibacterial substances. homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, bacilysin 100%. Conclusion subtilis determined through whole-genome analysis. Our study provides solid foundation development utilisation this strain.

Language: Английский

Citations

0