Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Nov. 18, 2022
Abstract
Background
.
Previous
evidence
suggested
sperm
Glutathione
S-transferase
Mu
3
(GSTM3)
to
be
essential
for
an
appropriate
mitochondrial
function,
plasma
membrane
stability
and
oxidative
regulation
of
mammalian
sperm.
In
humans,
however,
neither
has
this
enzyme
been
related
semen
alterations
nor
it
reported
associated
DNA
damage
(in)fertility.
Methods
The
aim
the
present
study
was
assess
utility
GSTM3
predict
spermiogram
in
A
total
34
samples
were
collected,
evaluated
by
conventional
analysis,
subsequently
cryopreserved.
Frozen-thawed
assessed
fragmentation
presence,
localization
content
GSTM3.
Results
Sperm
positively
quality
parameters
(
P
<
0.05).
Specifically,
lower
levels
antioxidant
observed
asthenoteratozoospermic
oligoasthenoteratozoospermic
men
0.05),
but
not
idiopathic
infertile
patients
>
when
compared
normospermic
samples.
Moreover,
negatively
Finally,
ability
determined
ROC
curve
analysis
using
alone
(AUC
0.89;
combination
with
0.91;
Conclusions
Although
limited
sample
size
warrants
further
studies
clinical
trials
larger
sets,
our
results
set
ground
as
a
novel
biomarker
therapy
target
male
infertility
Antioxidants,
Journal Year:
2022,
Volume and Issue:
11(1), P. 167 - 167
Published: Jan. 16, 2022
The
pathophysiology
of
male
infertility
involves
various
interlinked
endogenous
pathways.
About
50%
the
cases
in
men
are
idiopathic,
and
oxidative
stress
(OS)
reportedly
serves
as
a
central
mechanism
impairing
fertility
parameters.
antioxidant
system
operates
to
conserve
seminal
redox
homeostasis
required
for
normal
reproduction.
OS
strikes
when
generation
reactive
oxygen
species
(ROS)
overwhelms
capacity.
Thus,
treatment
finds
remarkable
relevance
case
idiopathic
or
subfertility.
However,
due
lack
proper
detection
infertility,
use
antioxidant(s)
some
may
be
arbitrary
lead
overuse
induction
'reductive
stress'.
Moreover,
inflammation
is
closely
linked
establish
vicious
loop
that
capable
disruption
reproductive
tissues.
result
exaggeration
cellular
damage
Therefore,
limitations
therapy
treating
failure
selection
specific
treatments
targeting
simultaneously,
two
core
mechanisms
infertility.
present
review
aims
elucidate
paradox
treatment,
from
viewpoints
both
reductive
well
overlooking
inflammatory
consequences.
Journal of Clinical Medicine,
Journal Year:
2023,
Volume and Issue:
12(21), P. 6775 - 6775
Published: Oct. 26, 2023
Background:
The
aim
of
this
case–control
study
is
to
investigate
possible
associations
between
GSTM1
polymorphism
and
redox
potential
with
sperm
parameters.
Methods:
group
consisted
samples
from
51
infertile
men
according
the
WHO
guidelines.
control
included
39
normal
seminal
DNA
was
extracted
genotyped
for
detection
polymorphism.
An
evaluation
static
(sORP)
using
MiOXSYSTM
system
conducted.
Results:
frequency
GSTM1-null
genotype
higher
in
male
individuals
(60.78%)
than
controls
(41.03%)
associated
a
2.228-fold
increased
risk
infertility.
Fertile
carrying
presented
lower
percentage
typical
morphology
slow
progressive
motility.
excess
observed
males
compared
fertile
ones.
In
sORP
values
had
positive
correlation
immotility
negative
regarding
total
count,
concentration,
Conclusions:
present
highlights
that
affect
both
males.
Moreover,
levels
could
be
used
as
an
additional
indicator
along
routine
semen
analysis
comprehensive
screening
men.
Frontiers in Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
9
Published: Feb. 25, 2021
Fifty
percent
of
male
subfertility
diagnosis
is
idiopathic
and
usually
associated
with
genetic
abnormalities
or
protein
dysfunction,
which
are
not
detectable
through
the
conventional
spermiogram.
Glutathione
S
-transferases
(GSTs)
antioxidant
enzymes
essential
for
preserving
sperm
function
maintaining
fertilizing
ability.
However,
while
role
GSTP1
in
cell
signaling
regulation
via
inhibition
c-Jun
N-terminal
kinases
(JNK)
has
been
enlightened
somatic
cells,
it
never
investigated
mammalian
spermatozoa.
In
this
regard,
a
comprehensive
approach
immunoblotting,
immunofluorescence,
computer-assisted
assessment
(CASA),
flow
cytometry
analysis
was
used
to
characterize
molecular
GSTP1–JNK
heterocomplex
physiology,
using
pig
as
model.
Immunological
assessments
confirmed
presence
localization
cells.
The
pharmacological
dissociation
resulted
activation
JNK,
led
significant
decrease
viability,
motility,
mitochondrial
activity,
plasma
membrane
stability,
well
an
increase
intracellular
superoxides.
No
effects
calcium
levels
acrosome
integrity
were
observed.
conclusion,
present
work
demonstrated,
first
time,
deactivating
crucial
maintain
also
set
grounds
understand
relevance
physiology.
Environmental Toxicology,
Journal Year:
2023,
Volume and Issue:
38(12), P. 2926 - 2938
Published: Aug. 11, 2023
Abstract
Environmental
hazards
are
an
increasing
concern
due
to
the
rapid
pace
of
industrialization.
Among
these
hazards,
noise
and
carbon
monoxide
(CO)
common
risk
factors
have
been
shown
cause
serious
health
problems.
However,
existing
studies
focused
on
individual
effects
CO
exposure
combined
two
remain
poorly
understood.
Our
study
aimed
examine
testicular
function
by
constructing
models.
findings
indicated
that
was
associated
with
a
higher
damage
male
reproductive
when
compared
alone.
This
evidenced
poorer
semen
quality
more
severe
pathological
testis.
led
levels
oxidative
stress
apoptosis
in
testes,
bioinformatics
analyses
suggesting
signaling
pathways
involved
responses.
Specifically,
activation
P53
pathway
found
contribute
caused
exposure.
Encouragingly,
pterostilbene
(PTE),
novel
phytochemical,
alleviated
exposure‐induced
reducing
germ
cell
apoptosis.
Overall,
we
identified
joint
toxicity
resulting
from
CO,
PTE
is
promising
potential
treatment
for
injuries
factors.
The
cover
image
based
Research
Article
Effects
possible
mechanisms
system
rats
Yingqing
Li
et
al.,
https://doi.org/10.1002/tox.23927
.
