Identification of a Cluster of Benzene Activation Enzymes in a Strictly Anoxic Methanogenic Consortium DOI Creative Commons
Courtney R. A. Toth, Olivia Molenda, Camilla Nesbø

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

The Oil Refinery (OR) consortium is a model methanogenic enrichment culture for studying anaerobic benzene degradation. Over 50% of the culture′s bacterial community comprised two closely related strains benzene-fermenting Desulfobacterota (designated ORM2a and ORM2b) whose mechanism degradation unknown. Two new metagenomes, including complete circularized metagenome-assembled genome (MAG) ORM2a, enabled thorough investigation this proteome. Among proteins identified were Bam-like subunits an ATP-independent benzoyl-CoA pathway associated downstream beta-oxidation producing acetyl-CoA. most abundant mapped to gene clusters unknown function. Syntenic in other known degrader, ORM2b Pelotomaculum candidate BPL, nine contigs assembled from hydrothermal vent metagenomes. Extensive searches against reference sequence structural databases indicate that first (″Magic″) cluster likely catalyzes chemically difficult activation step. second (″Nanopod″) predicted code efflux system may pump excess out cells mitigate toxicity. Phylogenomic analyses place within novel genus benzene-degrading specialists which we propose naming ″Candidatus Benzenivorax″.

Language: Английский

Identification of a Cluster of Benzene Activation Enzymes in a Strictly Anoxic Methanogenic Consortium DOI Creative Commons
Courtney R. A. Toth, Olivia Molenda, Camilla Nesbø

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

The Oil Refinery (OR) consortium is a model methanogenic enrichment culture for studying anaerobic benzene degradation. Over 50% of the culture′s bacterial community comprised two closely related strains benzene-fermenting Desulfobacterota (designated ORM2a and ORM2b) whose mechanism degradation unknown. Two new metagenomes, including complete circularized metagenome-assembled genome (MAG) ORM2a, enabled thorough investigation this proteome. Among proteins identified were Bam-like subunits an ATP-independent benzoyl-CoA pathway associated downstream beta-oxidation producing acetyl-CoA. most abundant mapped to gene clusters unknown function. Syntenic in other known degrader, ORM2b Pelotomaculum candidate BPL, nine contigs assembled from hydrothermal vent metagenomes. Extensive searches against reference sequence structural databases indicate that first (″Magic″) cluster likely catalyzes chemically difficult activation step. second (″Nanopod″) predicted code efflux system may pump excess out cells mitigate toxicity. Phylogenomic analyses place within novel genus benzene-degrading specialists which we propose naming ″Candidatus Benzenivorax″.

Language: Английский

Citations

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