Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 1054 - 1054

Published: June 18, 2024

Over the past decade, development of three-dimensional (3D) models has increased exponentially, facilitating unravelling fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues organs respond to biochemical biophysical stimuli under both physiological pathological conditions. This section presents a concise overview most recent updates on significant contribution different types 3D cell cultures including spheroids, organoids organ-on-chip bio-printed in advancing our understanding molecular mechanisms. The case studies presented include breast cancer (BC), endometriosis, liver microenvironment infections. In BC, establishment culture permitted visualization role cancer-associated fibroblasts delivery exosomes, as well significance physical properties extracellular matrix promoting proliferation invasion. approach also become valuable tool gaining insight general specific drug resistance. Given considerable heterogeneity offer more accurate representation vivo microenvironment, thereby identification translation novel targeted therapeutic strategies. advantages provided hepatic environment, conjunction high throughput characterizing various platforms, have enabled elucidation complex underlying threatening diseases. A limited number for gut skin infections been developed. However, profound comprehension spatial temporal interactions between microbes, host their environment may facilitate advancement vitro, ex disease models. Additionally, it pave way approaches diverse research fields. interested reader will find concluding remarks challenges prospects using discovering areas covered this review.

Language: Английский

---

A novel approach for engineering DHCM/GelMA microgels: application in hepatocellular carcinoma cell encapsulation and chemoresistance research

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 14, 2025

Liver cancer, a highly aggressive malignancy, continues to present significant challenges in therapeutic management due its pronounced chemoresistance. This resistance, which undermines the efficacy of conventional chemotherapy and targeted therapies, is driven by multifaceted mechanisms, with increasing emphasis placed on protective role tumor microenvironment (TME). The hepatocellular carcinoma extracellular matrix (ECM), primary non-cellular component TME, has emerged as critical regulator cancer progression drug particularly cell (HCC). In this study, hybrid biomimetic hydrogel was engineered integrating decellularized (DHCM) gelatin methacrylate (GelMA) precursors. composite DHCM/GelMA designed replicate physicochemical functional properties ECM, thereby offering platform explore interactions between HCCs their microenvironment. Leveraging custom-designed microfluidic 3D printing platform, we achieved high-throughput fabrication HCC-encapsulated microgels, characterized enhanced uniformity, biocompatibility, scalability. These microgels facilitated construction microtissues, were subsequently employed for chemoresistance studies. Our findings revealed that closely mimic microenvironment, effectively recapitulating key features ECM-mediated resistance. Mechanistic studies further demonstrated DHCM significantly upregulates expression Aquaporin 3 (AQP3) encapsulated HCCs. upregulation potentially activates mTOR signaling-associated autophagy pathways, enhancing models provide robust versatile studying underlying mechanisms resistance evaluating interventions. innovative approach highlights potential transformative tool cancer-associated tissue engineering anticancer screening. By enabling detailed investigations into ECM chemoresistance, study contributes advancing research offers promising strategies overcome ultimately improving clinical outcomes liver treatment.

Language: Английский

---

Microfluidic manipulation of the multicellular spheroids and organoids for modeling human diseases

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113718 - 113718

Published: April 1, 2025

Language: Английский

---

Tumoroids, a valid preclinical screening platform for monitoring cancer angiogenesis

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 26, 2024

In recent years, biologists and clinicians have witnessed prominent advances in vitro 3D culture techniques related to biomimetic human/animal tissue analogs. Numerous data confirmed that unicellular multicellular (tumoroids) tumor spheroids with dense native cells certain matrices are sensitive valid analytical tools for drug screening, cancer cell dynamic growth, behavior, etc. laboratory settings. Angiogenesis/vascularization is a very critical biological phenomenon support oxygen nutrients within the deep layer of solid masses. It has been shown endothelial (EC)-incorporated or -free spheroid/tumoroid systems provide relatively reliable platform monitoring formation nascent blood vessels micron/micrometer scales. Besides, paracrine angiogenic activity can be monitored after being treated different therapeutic approaches. Here, we aimed collect findings angiogenesis using spheroids. Vascularized spheroids/tumoroids help us elucidation mechanisms formation, development, metastasis by main influencing factors.

Language: Английский

---

The Biological Macromolecules constructed Matrigel for cultured organoids in biomedical and tissue engineering

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 247, P. 114435 - 114435

Published: Dec. 7, 2024

Language: Английский

---