3D spheroid model of adipose-derived stem cell and breast cancer cell co- culture for mimicking cell migration and the cancer environment DOI Creative Commons
Dong-Woo Kim, Changheon Kim,

Chaewon Bae

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Traditional two-dimensional (2D) cell culture methodologies exhibit significant limitations, notably their failure to replicate the intricate three-dimensional (3D) milieu of human tissue architecture. This discrepancy has catalyzed exploration advanced drug screening platforms that more faithfully mimic complex 3D biological environment. To this end, precision medicine research increasingly used in vitro models emulate microenvironment body. In study, breast cancer lines (MDA-MB-231 and SK-BR-3) were co-cultured with adipose-derived stem cells (ADSCs), mimicking tumor multicellular heterogeneous nature solid tumors compared a 2D only system. Additionally, collagen type I was within spheroid platform, enhancing accuracy platform characteristics. Significantly, interactions between ADSCs two markedly influenced factors such as elongation, molecular expression, migration patterns, sensitivity. The integration pivotal simulating microenvironment, highlighting even same line, varying microenvironmental contexts can lead vastly different experimental results. Thus, study provides insights into role cellular substrates akin body when constructing system based on cells.

Language: Английский

3D spheroid model of adipose-derived stem cell and breast cancer cell co- culture for mimicking cell migration and the cancer environment DOI Creative Commons
Dong-Woo Kim, Changheon Kim,

Chaewon Bae

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Traditional two-dimensional (2D) cell culture methodologies exhibit significant limitations, notably their failure to replicate the intricate three-dimensional (3D) milieu of human tissue architecture. This discrepancy has catalyzed exploration advanced drug screening platforms that more faithfully mimic complex 3D biological environment. To this end, precision medicine research increasingly used in vitro models emulate microenvironment body. In study, breast cancer lines (MDA-MB-231 and SK-BR-3) were co-cultured with adipose-derived stem cells (ADSCs), mimicking tumor multicellular heterogeneous nature solid tumors compared a 2D only system. Additionally, collagen type I was within spheroid platform, enhancing accuracy platform characteristics. Significantly, interactions between ADSCs two markedly influenced factors such as elongation, molecular expression, migration patterns, sensitivity. The integration pivotal simulating microenvironment, highlighting even same line, varying microenvironmental contexts can lead vastly different experimental results. Thus, study provides insights into role cellular substrates akin body when constructing system based on cells.

Language: Английский

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