Cellular Signalling, Journal Year: 2025, Volume and Issue: 132, P. 111817 - 111817
Published: April 16, 2025
Language: Английский
Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
ABSTRACT Introduction Berberis fortunei Lindl. (BF) is a medicinal plant widely utilized in East Asia. However, the chemical components present its roots, stems, and leaves have not been systematically analyzed compared. The specific active ingredients that inhibit HT29 colorectal cancer cells are still unclear. Objective aim of this study to comprehensively analyze compositions BF's evaluate their biological function against cells. Methodology GC‐IMS LC‐QTOF‐MS were employed volatile nonvolatile BF, respectively. MTT assay was used inhibitory effects extracts compounds from BF on A network analysis based molecular docking conducted identify potential targets compounds. Results total 77 116 identified BF. activity different parts followed order: roots > stems leaves. Protoberberine‐type alkaloids showed more pronounced at 24 h, whereas bisbenzylisoquinoline‐type demonstrated stronger 48 h. Network revealed significant differences pathways targeted by two types alkaloids. Conclusion This only but also examined inhibiting cells, laying theoretical foundation for further development application. findings provide diverse lead subsequent drugs cancer.
Language: Английский
Citations
0
BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: March 6, 2025
Colorectal cancer (CRC) represents a major malignancy that poses significant threat to human health worldwide. The establishment of reliable and pathologically relevant orthotopic model CRC is crucial for gaining deeper understanding its molecular mechanisms developing more effective therapies. Nonetheless, the development such models fraught with challenges primarily owing technical complexities associated transplantation cells into intestinal epithelium. luminal surface cecum was externalized visualize entire process involved in cecal epithelium BALB/c athymic nude mice. mechanically removed, preserving integrity submucosal layer. Caco-2 were subsequently inoculated onto epithelium-depleted reproduce within epithelial successful removal verified through use appropriate fluorescent labeling techniques examination fluorescence stereoscopic microscope. Following transplantation, formed tumors cecum, where progressed from flat monolayer thickened aberrant crypt foci, then protruding polyps, aided by mesenchymal infiltrating form stalk region, eventually large invading submucosa. Throughout this process, retained stem cell fetal signatures, regardless their location or proliferative status. Histopathological analysis further suggested interactions between transplanted surrounding normal play critical roles tumor elimination model. This study established novel mouse cecum. Tumor progression include sequential morphological changes invasive tumors. model, which mimics natural microenvironment, provides new opportunities investigate underlying evaluate anticancer therapies contexts.
Language: Английский
Citations
0
Cancer Treatment and Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 100899 - 100899
Published: March 1, 2025
To investigate the effect and molecular mechanism of ezetimibe on colorectal cancer (CRC), our study found that significantly inhibited proliferation progression CRC. Further showed RPS6KA2 might be target gene treatment expression was lower in human CRC tissue samples associated with T classification vascular invasion tumor cells. proliferation, migration, The underlying mechanisms indicated interaction between PCSK9 observed within cytoplasmic compartment suppressed MAPK signaling pathway BI-D1780 which is an inhibitor increased related proteins SW620 However, or stimulator did not affect expression, respectively. In vivo, cells overexpression could inhibit promote growth metastasis, promoted higher N TNM stage conclusion, firstly suggests suppresses by upregulating while downregulating PCSK9/MAPK pathway.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 17, 2025
5-Fluorouracil (5-FU)-based chemotherapy often leads to drug resistance and adverse reactions in individuals with colorectal cancer (CRC). Carrimycin (CAM), a notable antitumour effects across various tumour types, including hepatocellular carcinoma, glioblastoma, small-cell lung offers an alternative owing its limited side effects. Combination therapy is common strategy mitigate the negative of 5-FU enhance therapeutic efficacy. This study aimed investigate potential synergistic CAM on HCT-15 HT-29 CRC cell lines. Using computational analysis, we identified quantified 5-FU. The combination significantly outperformed alone inhibiting proliferation, colony formation, cycle progression, migration. Additionally, it markedly increased levels reactive oxygen species induced DNA damage. Furthermore, RNA-seq analysis revealed that JNK p38 MAPK signalling pathways were activated by this combination. In addition, validated mouse subcutaneous graft model. conclusion, enhances sensitivity cells both vitro vivo, suggesting as promising candidate for therapy.
Language: Английский
Citations
0
Artificial Intelligence Review, Journal Year: 2025, Volume and Issue: 58(6)
Published: March 17, 2025
Language: Английский
Citations
0
Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115848 - 115848
Published: March 1, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156674 - 156674
Published: March 1, 2025
Language: Английский
Citations
0
Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118014 - 118014
Published: March 31, 2025
Language: Английский
Citations
0
Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown
Published: March 31, 2025
Early diagnosis of colorectal cancer (CRC), a malignant tumor with high incidence and mortality rates worldwide, can significantly reduce both its mortality. Among diagnostic methods, fluorescence imaging provides non-invasive approach, eliminating the need for tissue biopsy minimizing patient discomfort. In this study, we identified water-soluble quinolinium molecular fluorescent probe (CYI), which exhibits dose-dependent quantum yield in PBS solution, reaching 5.96% at concentration 20 µM. The results demonstrated that CYI selectively enters CRC cells maintains stable intensity within them by specifically targeting mitochondria lysosomes, leading to accumulation enhanced intracellular fluorescence. Importantly, toxicity assays cellular animal levels confirmed is highly biocompatible doses, no toxic effects observed normal or organisms. This study identifies as biosafety profile, excellent stability, preferential uptake cells, demonstrating strong potential early screening vivo monitoring.
Language: Английский
Citations
0
Asian Journal of Surgery, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0