The determination of fibroblast and keratinocyte death types after their transplantation into γ-irradiated porous scaffold in vitro DOI Creative Commons
Yurii Kot, Kateryna Kot,

Рашад Афатович Курбанов

et al.

Regulatory Mechanisms in Biosystems, Journal Year: 2023, Volume and Issue: 14(4), P. 552 - 558

Published: Oct. 18, 2023

In the course of radiation therapy, normal cells surrounding tumor are also irradiated. During and after irradiation, they undergo a series structural metabolic changes, which can lead to cell death or transformation. Therefore, when planning conducting effects on taken into account with aim predicting further correcting post-radiation complications, including development burns ulcers. Radiation skin characterized by prolonged wound healing process, is accompanied sharp decrease in number viable affected tissue from first hours irradiation. The type significantly impact effectiveness therapy complication correction. it important study their irradiated three-dimensional culture model dermal equivalent, widely used today for modeling biological processes. To detect pathways death, levels reactive oxygen species, viability, undergoing autophagy, apoptosis, necrosis, content active caspases 3, 8, 9 was fluorometrically measured 3D laser scanning confocal microscopy. It determined that transplantation fibroblasts keratinocytes equivalent contributed an increase overall viability led significant concentration free forms equivalent. Cells within were not evenly distributed terms quantity count over time. A cluster higher formed around transplant. At same time, transplant found be more resistant cytotoxic factors post-irradiation environment compared keratinocytes. demonstrated non-irradiated predominantly through primarily introduction transplant, occurs apoptosis. culture, both without transplantation, there effector caspase 3. apoptosis mitochondrial mechanism (with predominance 9), while receptor-mediated dominates 8). obtained results new effective methods burns, chronic ulcers wounds various etiologies.

Language: Английский

An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects DOI Creative Commons
Jessica A. Keane, Alan D. Ealy

Animals, Journal Year: 2024, Volume and Issue: 14(2), P. 330 - 330

Published: Jan. 21, 2024

The in vitro production (IVP) of bovine embryos has gained popularity worldwide and recent years its use for producing from genetically elite heifers cows surpassed the conventional superovulation-based embryo schemes. There are, however, several issues with IVP that remain unresolved. One limitation special concern is low efficiency embryos. Exposure to reactive oxygen species (ROS) one reason why diminished. These highly molecules are generated small amounts through normal cellular metabolism, but their abundances increase culture because oocyte exposure temperature fluctuations, light exposure, pH changes, atmospheric tension, suboptimal media formulations, cryopreservation. When uncontrolled, ROS produce detrimental effects on structure function genomic mitochondrial DNA, alter DNA methylation, lipid membrane damage, modify protein activity. Several intrinsic enzymatic pathways control abundance antioxidants react reduce potential ROS. This review will focus exploring supplementing these antioxidant maturation, sperm viability, fertilization, culture.

Language: Английский

Citations

18
Manipulation of Oxygen Tension in Damaged Regions via Hypoxia‐Induced IPN Hydrogel Microspheres for Intervertebral Disc Regeneration DOI Creative Commons

Xingdie Zhou,

Zhendong Lv,

Zehao Chen

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Disruption of low oxygen tension homeostasis during intervertebral disc degeneration inhibits endogenous stem cell viability and function, posing a challenge for regeneration. Here, to achieve sustained hypoxia manipulation, constructed hypoxia‐inducible interpenetrating polymer network (IPN) hydrogel microspheres (HIMS) are by microfluidics integrate the hypoxic system with stabilizing network. The IPN is synthesized through two‐step polymerization process, consisting rapid photo‐crosslinked gelatin methacrylate anhydride (GM) I slow enzyme‐crosslinked vanillin‐grafted (GV) II. enzymatic reaction between GV laccase able create microenvironment modulate in situ within injured region. HIMS can reduce microenvironmental 1/3 maintain up 5 days, thereby activating PI3K/AKT/HIF‐1α signaling pathway cells promote differentiation into nucleus pulposus‐like cells. Additionally, NSC‐Exos loaded onto trigger progenitor/stem recruitment migration. Both vitro vivo assays demonstrate that NSC‐Exos@HIMS facilitates recruitment, targets differentiation, stimulates extracellular matrix synthesis. Overall, established herein provide novel strategy manipulating enhancing tissue regeneration regions degeneration.

Language: Английский

Citations

0
Hyperoxia and brain: the link between necessity and injury from a molecular perspective DOI
Richard Simon Machado, Khiany Mathias, Larissa Joaquim

et al.

Neurotoxicity Research, Journal Year: 2024, Volume and Issue: 42(2)

Published: April 1, 2024

Language: Английский

Citations

3
Early short-term hypoxia promotes epidermal cell migration by activating the CCL2-ERK1/2 pathway and epithelial–mesenchymal transition during wound healing DOI Creative Commons
Linbo Jin,

Shiqi Zhou,

Shihan Zhao

et al.

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract Background Due to vasculature injury and increased oxygen consumption, the early wound microenvironment is typically in a hypoxic state. We observed enhanced cell migration ability under short-term hypoxia. CCL2 belongs CC chemokine family was found be wounds enriched extracellular signal-regulated kinase (ERK)1/2 pathway our previous study. However, underlying mechanism through which CCL2-ERK1/2 regulates healing hypoxia remains unclear. Activation of epithelial–mesenchymal transition (EMT) key process cancer metastasis, during epithelial cells acquire characteristics mesenchymal enhance motility ability. relationship between EMT has yet explored. Methods HaCaT were cultured verify effect on scratch assays. Lentiviruses with silenced or overexpressed used explore An acute full-thickness cutaneous rat model established application an ERK inhibitor reveal hidden role ERK1/2 stage healing. The verified all above experiments western blotting. Results In study, we that promoted migration. Mechanistically, mediating CCL2. Overexpression via lentivirus migration, while silencing inhibited production related downstream proteins. addition, pathway, vivo vitro upstream relationships ERK1/2. Western blot results both demonstrated promotes epidermal by activating Conclusions Our work serves as stimulus for triggering EMT, promote accelerate closure. These findings provide additional detailed insights into new targets clinical treatment.

Language: Английский

Citations

3
Mechanism insights into the pleiotropic effects of nobiletin as a potential therapeutic agent on non-alcoholic fatty liver disease (NAFLD) DOI Open Access

Qiqi Ran,

Qianrong Gan,

Ye Zhu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 173, P. 116322 - 116322

Published: Feb. 23, 2024

Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases and emerging as fastest-growing causes liver-related deaths worldwide. It necessary to find strategies effectively prevent treat NAFLD, no definitive drug has been approved. Nobiletin (NOB) critical active ingredient Chinese herbal medicines such Citrus aurantium Citri Reticulatae Pericarpium, which have anti-inflammatory, antioxidant, lipid regulating, insulin resistance regulating effects. Numerous studies demonstrated that NOB can onset progression NAFLD. In this review, mechanisms for treating NAFLD summarized, hoping provide a basis subsequent research ground development therapeutic drugs

Language: Английский

Citations

2
Nanodelivery of antioxidant Agents: A promising strategy for preventing sensorineural hearing loss DOI Creative Commons

Zeinab Hajmohammadi,

Zohreh Bagher, Farzad Taghizadeh‐Hesary

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2024, Volume and Issue: 202, P. 114393 - 114393

Published: July 9, 2024

Language: Английский

Citations

2
Ferroptosis plays a crucial role in lung cell damage caused by ventilation stretch DOI
Wei Jiang, Jing Liu, Jingang Cui

et al.

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 209, P. 84 - 95

Published: Oct. 10, 2023

Language: Английский

Citations

3
The determination of fibroblast and keratinocyte death types after their transplantation into γ-irradiated porous scaffold in vitro DOI Creative Commons
Yurii Kot, Kateryna Kot,

Рашад Афатович Курбанов

et al.

Regulatory Mechanisms in Biosystems, Journal Year: 2023, Volume and Issue: 14(4), P. 552 - 558

Published: Oct. 18, 2023

In the course of radiation therapy, normal cells surrounding tumor are also irradiated. During and after irradiation, they undergo a series structural metabolic changes, which can lead to cell death or transformation. Therefore, when planning conducting effects on taken into account with aim predicting further correcting post-radiation complications, including development burns ulcers. Radiation skin characterized by prolonged wound healing process, is accompanied sharp decrease in number viable affected tissue from first hours irradiation. The type significantly impact effectiveness therapy complication correction. it important study their irradiated three-dimensional culture model dermal equivalent, widely used today for modeling biological processes. To detect pathways death, levels reactive oxygen species, viability, undergoing autophagy, apoptosis, necrosis, content active caspases 3, 8, 9 was fluorometrically measured 3D laser scanning confocal microscopy. It determined that transplantation fibroblasts keratinocytes equivalent contributed an increase overall viability led significant concentration free forms equivalent. Cells within were not evenly distributed terms quantity count over time. A cluster higher formed around transplant. At same time, transplant found be more resistant cytotoxic factors post-irradiation environment compared keratinocytes. demonstrated non-irradiated predominantly through primarily introduction transplant, occurs apoptosis. culture, both without transplantation, there effector caspase 3. apoptosis mitochondrial mechanism (with predominance 9), while receptor-mediated dominates 8). obtained results new effective methods burns, chronic ulcers wounds various etiologies.

Language: Английский

Citations

1