Potential roles of extracellular non–coding RNAs in pain treatment DOI Open Access

Motoyo Maruyama,

Hidenori Suzuki, Atsushi Sakai

et al.

PAIN RESEARCH, Journal Year: 2022, Volume and Issue: 37(2), P. 89 - 96

Published: July 15, 2022

Non–coding RNAs, including microRNA and lncRNA, affect various cellular functions primarily by regulating diverse stages of gene expression, such as transcription, translation, epigenetic modulation. In recent years, it has been shown that many non–coding RNAs are dysregulated in pain disorders can be a potential therapeutic target. Intriguingly, part encapsulated extracellular vesicles abundantly released into the space. These taken up nearby or even distant cells exert their own functions, indicating they act important mediators cell–cell communication. fact, some microRNAs have to cause hyperalgesia acting neurons immune cells. addition, blood cerebrospinal fluid expected biomarker for purposes, diagnosis, drug responsiveness prediction prognosis, disorders. this review, we summarize current insights significance RNA treatment.

Language: Английский

MicroRNA in intestinal tight junction regulation DOI Creative Commons
Manmeet Rawat, Prem Kumar Govindappa, Yash Gupta

et al.

npj gut and liver., Journal Year: 2025, Volume and Issue: 2(1)

Published: May 5, 2025

Language: Английский

Citations

0
MicroRNAs induced by Listeria monocytogenes and their role in cells DOI
Javad Allahverdy, Niloufar Rashidi

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 175, P. 105997 - 105997

Published: Jan. 18, 2023

Language: Английский

Citations

8
Cytolethal distending toxin B inoculation leads to distinct gut microtypes and IBS-D-like microRNA-mediated gene expression changes in a rodent model DOI Creative Commons
Gabriela Leite, Juliana de Freitas Germano,

Walter Morales

et al.

Gut Microbes, Journal Year: 2023, Volume and Issue: 16(1)

Published: Dec. 18, 2023

Diarrhea-predominant irritable bowel syndrome (IBS-D), associated with increased intestinal permeability, inflammation, and small bacterial overgrowth, can be triggered by acute gastroenteritis. Cytolethal distending toxin B (CdtB) is produced gastroenteritis-causing pathogens may underlie IBS-D development, through molecular mimicry vinculin. Here, we examine the effects of exposure to CdtB alone on gut microbiome composition, host gene expression, IBS-D-like phenotypes in a rat model. CdtB-inoculated rats exhibited anti-CdtB levels, which correlated stool wet weights, pro-inflammatory cytokines (TNFα, IL2) predicted microbial metabolic pathways including inflammatory responses, TNF diarrhea. Three distinct ileal profiles (microtypes) were identified rats. The first microtype (most like controls) had altered relative abundance (RA) genera Bifidobacterium, Lactococcus, Rothia. second lower diversity, higher Escherichia-Shigella RA, absolute E. coli abundance, tissue expression immune-response TNF-response genes compared controls. third RA hydrogen sulfide (H2S)-producer Desulfovibrio, H2S-associated pain/serotonin response genes. All decreased cell junction component mRNAs, vinculin-associated proteins. Significantly, cluster-specific microRNA-mRNA interactions controlling visceral hypersensitivity/pain, gastrointestinal motility genes, several previously IBS seen. These findings demonstrate that results IBS-like inflammation diarrhea-like stool, barrier components, microtypes influenced changes microRNA-modulated consistent specific symptoms.

Language: Английский

Citations

7
MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expression DOI Open Access

Jianguo Guo,

Yuefeng Rao, Jun Jiang

et al.

Annals of Translational Medicine, Journal Year: 2022, Volume and Issue: 11(2), P. 34 - 34

Published: Dec. 22, 2022

Irritable bowel syndrome (IBS) is a common gastrointestinal disease. Emerging studies have demonstrated that microRNAs (miRNAs) are commonly dysregulated in patients with IBS, and aberrant miRNAs implicated IBS occurrence. Although miR-155-5p participates inflammatory disease (IBD) intestinal barrier dysfunction, the role of unclear.In present study, colon samples were obtained from mice induced by trinitrobenzenesulfonic acid (TNBS), levels miR-155-5p, claudin-1 (CLDN1), zonula occludens-1 (ZO-1) assessed using quantitative real-time polymerase chain reaction (qRT-PCR) immunohistochemical analysis. The regulatory CLDN1 ZO-1 expression was validated dual luciferase reporter assay.We found upregulated compared healthy subjects normal mice, respectively. Meanwhile, decreased mice. Importantly, forced inhibited expression. In intraperitoneal injection inhibitor increased mucosal epithelium, enhanced visceral response thresholds, myeloperoxidase (MPO) activity.In summary, these results suggested participated pathogenesis at least part inhibiting expression, indicating may be potential therapeutic target for IBS.

Language: Английский

Citations

9
Integrated omics analysis reveals the epigenetic mechanism of visceral hypersensitivity in IBS-D DOI Creative Commons

Yaoyao Lu,

Yuna Chai,

Jianli Qiu

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 16, 2023

Background and objective: IBS-D is a common functional bowel disease with complex etiology without biomarker. The pathological physiological basis of focuses on visceral hypersensitivity. However, its epigenetic mechanism remains elusive. Our study aimed to integrate the relationship between differentially expressed miRNAs, mRNAs proteins in patients order reveal hypersensitivity from transcription protein levels provide molecular for discovering biomarkers IBS-D. Methods: intestinal biopsies healthy volunteers were obtained high-throughput sequencing miRNAs mRNAs. differential selected verified by q-PCR experiment followed target mRNA prediction. Biological functions respectively analyzed mRNAs, previously identified explore characteristic involved At last, interaction analysis was performed regulation levels. Results: Thirty-three found be five them further confirmed, including upregulated hsa-miR-641, hsa-miR-1843, hsa-let-7d-3p downregulated hsa-miR-219a-5p, hsa-miR-19b-1-5p. In addition, 3,812 identified. Thirty intersecting molecules Fourteen proteins, thirty-six different According integrated miRNA-mRNA-protein, we noticed two new COPS2 regulated hsa-miR-19b-1-5p MARCKS hsa-miR-641. Meanwhile some critical signaling pathways such as MAPK, GABAergic synapse, Glutamatergic Adherens junction. Conclusion: expressions hsa-let-7d-3p, tissues significantly different. Moreover, they could regulate variety pathways, which multifaceted multilevel

Language: Английский

Citations

4
Contribution of MicroRNAs in the Development of Irritable Bowel Syndrome Symptoms DOI Open Access

P Dupont,

Irma Yadira Izaguirre-Hernández,

J.M. Remes-Troche

et al.

Journal of Gastrointestinal and Liver Diseases, Journal Year: 2023, Volume and Issue: 32(2), P. 230 - 240

Published: June 22, 2023

Irritable bowel syndrome (IBS) is a common chronic functional gastrointestinal disorder defined by disturbances in habits and abdominal pain, the absence of known organic pathology that affects between 5 to 10% healthy populations. Despite improvements detection treatment, pathogenesis IBS has not been clarified. Several microRNAs (miRNAs) are involved through increased intestinal permeability, inflammation, modulation visceral hyperalgesia, they may have potential be used as biomarkers therapeutic targets. Here, we summarized recent advances about role miRNAs development symptoms possibility use them targets mitigate IBS.

Language: Английский

Citations

4
Drug Discovery and Development of miRNA-Based Nucleotide Drugs for Gastrointestinal Cancer DOI Creative Commons
Hiromichi Sato,

Tomoaki Hara,

Sikun Meng

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(8), P. 2235 - 2235

Published: Aug. 9, 2023

Short non-coding RNAs, miRNAs, play roles in the control of cell growth and differentiation cancer. Reportedly, introduction miRNAs could reduce biologically malignant behavior cancer cells, suggesting a possible use as therapeutic reagents. Given that forced expression several including miR-302, results cellular reprograming human mouse which is similar to effects transcription factors Oct4, Sox2, Klf4, c-Myc, this suggests selective will be able achieve anti-cancer at epigenetic metabolic levels. In review article, we bring together recent advances made studies microRNA-based approaches therapy-resistant cancers, especially gastrointestinal organs.

Language: Английский

Citations

3
Analyzing Protein-Lipid Interactions by Nuclear Magnetic Resonance (NMR) DOI
Suman Adhikari, Alakesh Das, Abhijit Datta

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Toll-like receptor 4 plays a vital role in irritable bowel syndrome: a scoping review DOI Creative Commons

Xue-Meng Wan,

Liyuan Wang,

Zhong Lin Wang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 19, 2024

Irritable bowel syndrome (IBS) is a common gastrointestinal disease. Recently, an increasing number of studies have shown that Toll-like receptor 4 (TLR4), widely distributed on the surface variety epithelial cells (ECs) and immune sentinel in gut, plays vital role developing IBS.

Language: Английский

Citations

0
MiR-595 and Cldnd1: Potential related factors for bone loss in postmenopausal women with hip osteoporotic fracture DOI Creative Commons

Jingyue Sun,

Peixin Liu, Wang Xiao

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313106 - e0313106

Published: Dec. 31, 2024

Background Recently researches have reported that miRNA and its target genes are associated with osteoporosis. MiRNA mRNA might be potential diagnostic markers for Purposes The aim of this study is to explore the by bioinformatics method clinical analysis. Patients methods expression profiles were obtained from GSE74209, GSE64433 GSE115773 in Gene Omnibus (GEO). GSE100609. Wayne intersection used different expressed miRNAs (DE-miRs). Select highest Fold Change subsequent research. Screening using TargetScan miRDB tools. GO KEGG analyses (TGs) function performed. Validate selected TGs We collected female patients femural intertrochanteric fractures July 1, 2023 October 31, 2023. Patient’s bone marrow data collected. differentially verified through RT-qPCR. All subjected Shapiro-Wilk test. Using Pearson or Spearman test detect correlation between various indicators, then incorporating indicators related density into multiple linear regression equations. Partial analysis was analyze final density. Results A total 140 DE-miRs identified. Set fold change “>1” ultimately include 5 miRNAs. miR-595 (highest |log 2 FC|) as subject 3542 targeted mRNAs predicted 362 miRDB. 337 intersected, which mainly enriched nucleus. Only Cldnd1 identified GSE100609 dataset. found highly high mass, while downregulated. There a strong collinearity Cldnd1. Further showed Conclusions These suggest factors decreased mass.

Language: Английский

Citations

0